Gamitromisin ve Tulatromisinin Sığır Trakea Düz Kası Üzerine Etkileri

Yıl 2020, Cilt: 31 Sayı: 2, 140 - 146, 31.12.2020

Yaşar Şahin , Ebru Yıldırım , Begüm Yurdakök Dikmen

https://doi.org/10.35864/evmd.762503

Cited By: 1

Öz

Bu çalışmada; sığır solunum yolu hastalıklarında yaygın olarak kullanılan gamitromisin ve tulatromisinin, erkek Simental ırkı sığır trakea düz kası üzerine etkilerinin izole organ banyosunda araştırılması ve bu iki makrolid antibiyotiğin etkilerinin karşılaştırılması amaçlandı. İlk protokolde gamitromisin ve tulatromisin 10-7- 3×10-5 M derişimlerinde tek başına, trakea düz kası üzerine etkisiz olduğu bulundu. İkinci protokolde; 20 dakika 3×10-5 M gamitromisin veya tulatromisin ile inkübe edilen trakea düz kası üzerine kümülatif ACh (10-8-10-3 M) derişimleri uygulandı, elde edilen pD2 ve Emax değerleri arasında fark bulunamadı. Üçüncü protokolde; 65mM KCl ile ön kasılma oluşturulan trakea düz kası üzerine uygulanan gamitromisin ve tulatromisin (10-10-10-5 M) derişimlerinde bir fark bulunamadı. Asetilkolin EC85 ( 2,3×10-4 M) değeriyle ön kasılma oluşturulan trakea düz kası üzerine gamitromisin ve tulatromisinin etkilerinin araştırıldığı dördüncü protokolde; tulatromisinin (10-10-10-5 M) derişimlerinde kasılma veya gevşeme yanıtı oluşmadı. Buna karşın gamitromisinin 10-7 M (%5.45±1.13) ve 10-6 M (%8.51±1.69) derişimlerinde, kontrole göre istatistiksel olarak gevşeme cevapları tespit edildi (p<0.05). Dördüncü protokol sonucu yapılan son protokolde; gamitromisinin 10-6 M derişiminin inkübasyonunda; asetilkolin cevaplarının Emax değeri sayısal olarak azalmıştır. Sonuç olarak derişime bağlı şekilde gamitromisin; ACh ile ön kasılma oluşturulan dokularda az da olsa gevşeme etkisi göstermiş olup, bu etki özellikle astım ya da solunum yolunda ödemle seyreden hastalıklarda tedaviye katkı sağlayabilir.

Anahtar Kelimeler

Gamitromisin, izole organ banyosu, sığır, trakea düz kas, tulatromisin

Destekleyen Kurum

Kırıkkale Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

2018/047

Kaynakça

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