Multietiological abortion induced by Brucella melitensis and Chlamydophila abortus in a sheep fetus

Yıl 2023, Cilt: 34 Sayı: 2, 205 - 210, 28.12.2023

İbrahim Deniz , Ertan Oruç

https://doi.org/10.35864/evmd.1289382

Öz

In this case, multietiological abortion due to Brucella melitensis (B. melitensis) and Chlamydophila abortus (C. abortus) agents was defined in a sheep abortion. An aborted sheep fetus submitted to Konya Veterinary Control Institute (KVCI) from a sheep farm in Nigde province in Brucella spp. presence was determined by bacterial isolation method and confirmed by slide agglutination test with monospecific A and M sera. C. abortus was detected by real time PCR and immunohistochemistry (IHC) methods. In histopathological examinations, in the lung; hyperemia, hemorrhage and bronchopneumonia, In the heart; in the placenta with degeneration and necrosis of heart muscle cells; hyperemia, hemorrhage, edema, necrosis and mononuclear cell infiltrations were observed. In this study, it was emphasized that in the fight against sheep abortions, multi-etiological abortions involving more than one factor should be taken into consideration, as well as an etiological agent, and it would be appropriate to use multidisciplinary diagnostic methods to determine the abortion factors, thus contributing to the prevention of abortions, which is still an important problem in sheep breeding.

Anahtar Kelimeler

Brucella melitensis, Chlamydophila abortus, Immunohistochemistry, Multietiological abortion, PCR

Destekleyen Kurum

There is no person/organization that financially supports the study and the authors do not have any conflict of interest.

Proje Numarası

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Teşekkür

We would like to thank the Ministry of Agriculture and Forestry General Directorate of Food and Control for permission to publish the case report and the Konya Veterinary Control Institute Directorate for their support.

Bir koyun fetusunda Brucella melitensis ve Chlamydophila abortus tarafından oluşturulan multietiyolojik abort

Öz

Bu olguda, Konya Veteriner Kontrol Enstitüsü Müdürlüğüne (KVKEM) 2021 yılında, Niğde ilindeki bir koyun işletmesinden gönderilen koyun abortunda Brucella melitensis (B. melitensis) ve Chlamydophila abortus (C. abortus) etkenlerine bağlı multietiyolojik abort tanımlandı. Brucella spp. varlığı bakteriyel izolasyon yöntemi ile belirlendi ve monospesifik A ve M serumları ile yapılan lam aglutinasyon testi ile doğrulandı. C. abortus ise real time PZR ve immunohistokimya (İHK) yöntemleri ile tespit edildi. Histopatolojik incelemelerde ise akciğerde; hiperemi, kanama ve bronkopnömoni, Kalpte; kalp kası hücrelerinde dejenerasyon ve nekroz ile plasentada; hiperemi, kanama, ödem, nekroz ve mononükleer hücre infiltrasyonları gözlendi. Bu çalışmanın, koyun abortları ile mücadelede tek bir etiyolojik ajanın yanında birden fazla etkenin karıştığı multietiyolojik abortların da dikkate alınması gerektiği, abort etkenlerini belirlemek için de multidisipliner teşhis yöntemlerinin kullanılmasının uygun olacağını belirtmek ve böylelikle koyun yetiştiriciliğinde halen önemli bir sorun olan abortların önlenmesine katkı sağlaması açısından yayımlanması uygun bulunmuştur.

Anahtar Kelimeler

Brucella melitensis, Chlamydophila abortus, Immunohistochemistry, Multietiological abortion, PCR

Proje Numarası

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Kaynakça

• Akashi H, Onuma S, Nagano H, Ohta M, Fukutomi T. (1999) Detection and differentiation of Aino and Akabane Simbu serogroup bunyaviruses by nested polymerase chain reaction. Archives of virology. 144, 2101-2109.
• Ay SS. (2017) Küçük ruminantlarda abortus sorunu ve reprodüktif aşılama programları. Turkiye Klinikleri J Vet Sci Obstet Gynecol-Special Topics. 3,2, 129-136.
• Aydin F, Gumussoy, KS, Atabay HI, Iça T, Abay S. (2007) Prevalence and distribution of Arcobacter species in various sources in Türkiye and molecular analysis of isolated strains by ERIC‐PCR. Journal of applied microbiology. 103, 1, 27-35.
• Costa JM, Pautas C, Ernault P, Foulet F, Cordonnier C, Bretagne S. (2000) Real-time PCR for diagnosis and follow-up of Toxoplasma reactivation after allogeneic stem cell transplantation using fluorescence resonance energy transfer hybridization probes. Journal of clinical microbiology. 38, 8, 2929-2932.
• De Angelis ME, Martino C, Chiaverini A, Di Pancrazio C, Di Marzio V, Bosica S, Malatesta D, Salucci S, Sulli N, Acciari VA, Pomilio F. (2022) Co-Infection of L. monocytogenes and Toxoplasma gondii in a Sheep Flock Causing Abortion and Lamb Deaths. Microorganisms. 10, 8, 1647. https://doi.org/10.3390/microorganisms10081647.
• Dhama K, Karthik K, Tiwari R, Shabbir MZ, Barbuddhe S, Malik SVS, Singh RK. (2015) Listeriosis in animals, its public health significance (food-borne zoonosis) and advances in diagnosis and control: a comprehensive review. Veterinary Quarterly. 35, 4, 211-235.
• Filikci K. (2022) Pathomorphology of Sheep and Goat Chlamydial Abortions. PhD Thesis, Erişim Adresi: https://tez.yok.gov.tr/UlusalTezMerkezi/tezSorguSonucYeni.jsp AU Institute of Health Sciences, Ankara.
• Gulaydın O, Ozturk C, Ekin İH, İlhan Z, İlhan F. (2023) Investigation of selected bacterial agents causing sheep abortion in the Van Province by RT-PCR and histopathological methods. Acta Veterinaria Brno. 92, 1, 69-77.
• Hailat N, Shereen K, Mustafa A, Corrie B. (2018) Pathological, Immunohistochemical and Molecular Diagnosis of Abortions in Small Ruminants in Jordan with Reference to Chlamydia abortus and Brucella melitensis. Pakistan Veterinary Journal. 38, 01, 109-112.
• Hofmann M, Griot C, Chaignat V, Perler L, Thur B. (2008) Bluetongue disease reaches Switzerland. Schweizer Archiv fur Tierheilkunde. https://doi.org/10.1024/0036-7281.150.2.49, 150, 2, 49-56 .
• Kanat O. (2022) Molecular and histopathologic investigation of Pestivirus, Chlamydophila abortus and Listeria monocytogenes infections in aborted sheep foetuses. Journal of the Hellenic Veterinary Medical Society. 73, 1, 3889-3896.
• Li L, Wu X, Liu F, Wang Z, Liu C, Wang Q, Bao J. (2016) Rapid detection of lineage IV peste des petits ruminants virus by real-time RT-PCR. Journal of virological methods. 235, 131-133.
• Livingstone M, Wheelhouse N, Ensor H, Rocchi M, Maley S, Aitchison K, Wattegedera S, Wilson K, Sait M, Siarkou V, Vretou E, Entrican G, Dagleish M, Longbottom D. (2017) Pathogenic outcome following experimental infection of sheep with Chlamydia abortus variant strains LLG and POS. PLoS One. 12, 5, e0177653.
• Longbottom D, Livingstone M, Maley S, Van Der Zon A, Rocchi M, Wilson K, Wheelhouse N, Dagleish M, Aitchison K, Wattegedera S, Nath M, Entrican G, Buxton D. (2013) Intranasal infection with Chlamydia abortus induces dose-dependent latency and abortion in sheep. PLoS One. 8, 2, e57950.
• Malal ME, Turkyilmaz S. (2021) Identification and genotyping of Chlamydia abortus with MLVA from ruminant abortions in the Marmara region of Türkiye. The Thai Journal of Veterinary Medicine. 51, 1, 169-175.
• Maley SW, Livingstone M, Rodger SM, Longbottom D, Buxton D. (2009) Identification of Chlamydophila abortus and the development of lesions in placental tissues of experimentally infected sheep. Vet Microbiol. 135, 1-2, 122-127.
• Muller N, Zimmermann V, Hentrich B, Gottstein B. (1996) Diagnosis of Neospora caninum and Toxoplasma gondii infection by PCR and DNA hybridization immunoassay. Journal of Clinical Microbiology. 34, 11, 2850-2852. Pantchev A, Sting R, Bauerfeind R, Tyczka J, Sachse K. (2009) New real-time PCR tests for species-specific detection of Chlamydophila psittaci and Chlamydophila abortus from tissue samples. Vet J. 181, 2, 145-150.
• Ramo MD, LA, Benito AA, Quílez J, Monteagudo LV, Baselga C, Tejedor MT. (2022) Coxiella burnetii and Co-Infections with Other Major Pathogens Causing Abortion in Small Ruminant Flocks in the Iberian Peninsula. Animals. 12, 24, 3454.
• Sakmanoğlu A, Uslu A, Sayın Z, Golen GS, İlban A, Padron-Perez B, Karyeyen Y, Gök A, Tekindal MA, Erganis O. (2021) A one-year descriptive epidemiology of zoonotic abortifacient pathogen bacteria in farm animals in Türkiye. Comparative Immunology, Microbiology and Infectious Diseases. 77, 101665.
• Şevik M, Gulcu Y, Dogan M. (2017a) Dual Infection of Sheep Aborted Foetus with Peste des Petits Ruminants Virus and Brucella melitensis. Etlik Veteriner Mikrobiyoloji Dergisi. 28, 2, 61-64.
• Şevik M, Gulcu Y, Dogan M. (2017b) Co-infection with Border Disease Virus and Brucella melitensis in an aborted sheep foetus. Etlik Veteriner Mikrobiyoloji Dergisi. 28, 2, 65-68.
• Van den Brom R, Lievaart-Peterson K, Luttikholt S, Peperkamp K, Wouda W, Vellema P. (2012) Abortion in small ruminants in the Netherlands between 2006 and 2011. Tijdschrift voor diergeneeskunde. 137,7, 450-457.
• Vilček Š, Paton D. (2000) A RT-PCR assay for the rapid recognition of border disease virus. Veterinary Research. 31, 4, 437-445.
• Westermann T, Jenkins C, Onizawa E, Gestier S, McNally J, Kirkland P, Zhang J, Bogema D, Manning LK, Walker K, Pinczowski P. (2021) Chlamydia pecorum-Associated Sporadic Ovine Abortion. Vet Pathol. 58, 1, 114-122.
• Woah. (2022a) Brucellosis (infectıon with Brucella abortus, Brucella melitensis and Brucella suis). Date of access: 04.05.2023, Access address: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.01.04_BRUCELLOSIS.pdf
• Woah. (2022b) Salmonellosis. Date of access: 04.05.2023, Access address: https://www.woah.org/fileadmin/Home/eng/Health_standards/tahm/3.10.07_SALMONELLOSIS.pdf
• Wu Z, Sippy R, Sahin O, Plummer P, Vidal A, Newell D, Zhang Q. (2014) Genetic diversity and antimicrobial susceptibility of Campylobacter jejuni isolates associated with sheep abortion in the United States and Great
• Britain. Journal of clinical microbiology. 52, 6, 1853-1861. Yeni DK. (2022) Molecular diagnosis of neglected infectious agents of sheep and cattle abortions: the prevalences of Coxiella burnetii, Francisella tularensis and Chlamydophila abortus at a glance. Ankara Univ Vet Fak Derg. 69, 425-430.
• Yeşilmen S, Yaman T, Sağsöz H, Bademkıran S. (2018) Diagnosis of Q fever and Brucellosis in aborted ovine fetuses by microbiological, pathological and immunohistochemical methods. Acta Veterinaria-Beograd. 68, 2, 168-177.