White Cabbage Extract Reduces the Harmful Effects of Amiodarone on Heart Tissue in Rats

Yıl 2020, , 1 - 6, 13.03.2020

Hazal Hazineci İsmet Burcu Türkyılmaz Ünsal Veli Üstündağ , Ebru Emekli-alturfan Burçin Alev Tüzüner Tuğba Tunalı-akbay Refiye Yanardağ Ayşen Yarat

https://doi.org/10.26650/experimed.2020.0004

Öz

Objective: Amiodarone is used in the treatment of arrhythmia and has been shown to cause side effects in various organs. White cabbage (Brassica oleracea L. var. capitata) is a widely consumed vegetable. The effects of exposure to amiodarone and white cabbage consumption on heart tissue has not previously been investigated. We aimed to investigate the effects of white cabbage extract on amiodarone given to rats’ cardiac tissues. Material and Method: Rats were given white cabbage extract and amiodarone by gavage for seven days. Amiodarone was applied 1 hour after administration of the white cabbage extract. On the eighth day, the hearts of the animals were taken and homogenized. The levels of lipid peroxidation (LPO), reduced glutathione (GSH), sialic acid (SA) and activities of glutathione-S-transferase (GST), catalase, superoxide dismutase (SOD) and tissue factor (TF) were determined. Results: The GSH level, CAT and SOD activities decreased, while the LPO and SA levels increased in the amiodarone treated group. The White cabbage extract prevented any damage of the amiodarone by decreasing the amount of LPO and SA and also strengthened the antioxidant defence system by increasing the amount of the GSH and SOD activities and the CAT and GST activities. Conclusion: It is suggested that the consumption of white cabbage could be an alternative approach to the prevention of the adverse effects of amiodarone on heart tissue.

Anahtar Kelimeler

Amiodarone, side effect, white cabbage

Destekleyen Kurum

This study was supported by the Marmara University Scientific Research and Project Commision (Project No: SAGCYLP- 110315-0054).

Proje Numarası

Project No: SAGCYLP- 110315-0054

Beyaz Lahana Ekstresi Amiodaronun Sıçanlarda Kalp Dokusu Üzerindeki Zararlı Etkilerini İyileştirir

Öz

Amaç: Aritmi tedavisinde kullanılan amiodaronun çeşitli organlarda yan etkilere neden olduğu gösterilmiştir. Beyaz lahana (Brassica oleracea L. var. capitata) yaygın olarak tüketilen bir sebzedir. Amiodaron maruziyeti ve beyaz lahana tüketiminin kalp dokusu üzerindeki etkileri araştırılmamıştır. Beyaz lahana ekstraktının amiodaron uygulanan sıçanların kalp dokuları üzerindeki etkilerini araştırmayı amaçladık. Gereç ve Yöntem: Sıçanlara yedi gün boyunca gavajla beyaz lahana ekstresi ve amiodaron verildi. Beyaz lahana ekstresinin uygulanmasından 1 saat sonra amiodaron uygulandı. Sekizinci günde, hayvanların kalpleri alındı ve homojenize edildi. Lipit peroksidasyon (LPO), indirgenmiş glutatyon (GSH), siyalik asit (SA) düzeyleri ile glutatyon-S-transferaz (GST), katalaz, süperoksit dismutaz (SOD) ve doku faktörü (TF) aktiviteleri belirlendi. Bulgular: Amiodaron verilen grupta GSH, CAT, SOD aktiviteleri azalırken LPO ve SA seviyeleri arttı. Beyaz lahana, LPO ve SA miktarını azaltarak amiodaron zararını önledi. Ayrıca GSH düzeyleri ile SOD, CAT ve GST aktivitelerini artırarak antioksidan savunma sistemini güçlendirdi. Sonuç: Beyaz lahana tüketiminin, amiodaronun kalp dokusunda olumsuz etkilerini önlemek için alternatif bir yaklaşım olabileceği ileri sürülebilir.

Anahtar Kelimeler

Amiodaron, yan etki, beyaz lahana

Proje Numarası

Project No: SAGCYLP- 110315-0054

Kaynakça

• 1. Oliveira PF, Dias da Silva VJ, Salgado MCO, Fazan Jr. R, Aguiar CA, Salgado HC. Acute effect of amiodarone on cardiovascular reflexes of normotensive and renal hypertensive rats. Braz J Med Biol Res 2005; 38(6): 967-76. [CrossRef]
• 2. Hughes M, Binning A. Intravenous amiodarone in intensive care. Intensive Care Med 2000; 26: 1730-39. [CrossRef]
• 3. Rao RH, McCready VR, Spathis GS. Iodine kinetic studies during amiodarone treatment. J Clin Endocrinol Metab 1986; 62: 563-8. [CrossRef]
• 4. Narayana SK, Woods DR, Boos CJ. Management of amiodarone-related thyroid problems. TAEM 2011; 2(3): 115-26. [CrossRef]
• 5. Basaria S, Cooper DS. Amiodarone and the thyroid. Am J Med 2005; 118(7): 706-14. [CrossRef]
• 6. Bogazzi F, Bartalena L, Gasperi M, Braverman LE, Martino E. The various effects of amiodarone on thyroid function. Thyroid 2001; 11(5):511-9. [CrossRef]
• 7. Singh J, Upadhyay AK, Bahadur A, Singh B, Singh KP, Rai M. Antioxidant phytochemicals in cabbage (Brassica oleracea L. var. capitata) Sci Hortic 2006; 108(3): 233-37. [CrossRef]
• 8. Nieuwhof M. Cole Crops: Botany, Cultivation and Utilization. World Crops Series. Leonard Hill, London, UK, 1969; pp353.
• 9. DeFilipps RA, Krupnick GA. The medicinal plants of Myanmar. PhytoKeys 2018; 28;(102): 1-341. [CrossRef]
• 10. Wells PG, McCallum GP, Chen CS, Henderson JT, Lee CJ, Perstin J, et al. Oxidative stress in developmental origins of disease: teratogenesis, neurodevelopmental deficits, and cancer. Toxicol Sci 2009;108: 4-18. [CrossRef]
• 11. Lapenna D, Ciofani G, Bruno C, Pierdomenico SD, Cuccurullo F. Antioxidant activity of amiodarone on human lipoprotein oxidation. BJP 2001; 133(5): 739-45. [CrossRef]
• 12. Felser A, Blum K, Lindinger PW, Bouitbir J, Krähenbüh S. Mechanisms of hepatocellular toxicity associated with dronedarone--A comparison to amiodarone. Toxicol Sci 2013; 131(2): 480-90. [CrossRef]
• 13. Lewis JH, Ranard RC, Caruso A, Jackson LK, Mullick F, Ishak KG, et al. Amiodarone hepatotoxicity: Prevalence and clinicopathologic correlations among 104 patients. Hepatology 1989; 9: 679-85. [CrossRef]
• 14. Waldhauser KM, Török M, Ha HR, Thomet U, Konrad D, Brecht K, et al. Hepatocellular toxicity and pharmacological effect of amiodarone and amiodarone derivatives. J. Pharmacol Exp Ther 2006; 319: 1413-23. [CrossRef]
• 15. Brown DA, Perry JB, Allen ME, et al. Expert consensus document: Mitochondrial function as a therapeutic target in heart failure. Nat Rev Cardiol. 2017;14(4): 238-50. [CrossRef]
• 16. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ. Protein measurement with the Folin phenol reagent. J Biol Chem 1951; 193(1): 265-75.
• 17. Yagi K. Assay for blood plasma or serum. Methods Enzymol 1984; 105: 328-37. [CrossRef] 18. Beutler E. Gluthatione: red cell metabolism. A manual bio chemical methods. New York: Grune and Stratton; 1975.
• 19. Aebi H. Catalase in vitro. In: Bergmeye HU, editor. Methods of enzymatic analysis. Wenheim: Verlag Chemie; 1974. [CrossRef]
• 20. Mylorie AA, Collins H, Umbles C, Kyle J. Erythrocyte SOD activity and other parameters of copper status in rats ingesting lead acetate. Toxicol Appl Pharmacol 1986; 82: 512-20. [CrossRef]
• 21. Habig WH, Jacoby WB. Assays for differentation of glutathi one-Stransferases. Methods Enzymol 1981; 77: 398-405. [CrossRef]
• 22. Warren L. The thiobarbituric acid assay of sialic acids. J Biol Chem 1959; 234: 1971-75.
• 23. Ingram GIC, Hills M. Reference method for the one stage prothrombin time test on human blood. Thromb Haemostas 1976; 36: 237-8. [CrossRef]
• 24. Brooks JD, Paton VG, Vidanes G. Potent induction of phase 2 enzymes in human prostate cells by sulforaphane. Cancer Epidemiol Biomarkers Prev 2001; 10(9): 949-54.
• 25. Dekker M, Verkerk R, Jongen WM. Predictive modelling of health aspects in the food production chain: a case study on glucosinolates in cabbage. Trends Food Sci 2000; 11: 174-81. [CrossRef]
• 26. Ji C, Li C, Gong W, Niu H, Huang W. Hypolipidemic action of hydroxycinnamic acids from cabbage (Brassica oleracea L. var. capitata) on hypercholesterolaemic rat in relation to its antioxidant activity. J Food Nutr Res 2015; 3(5): 317-24. [CrossRef]
• 27. Simin N, Orcic D, Cetojevic-Simin D, Mimica-Dukic N, Anackov G, Beara I, Bozin B. Phenolic profile, antioxidant, anti-inflammatory and cytotoxic activities of small yellow onion (Allium flavum L. subsp. flavum, Alliaceae). LWT-Food Sci Technol 2013; 54(1): 13946. [CrossRef]
• 28. Chakraborty A, Mondal C, Sinha S, Mandal J, Chandra AK. Amiodarone induced oxidative stress in stress - vulnerable organs of adult male rats. Asian J Pharm Clin Res. 2014; 7: 4:177-83.
• 29. Ponnio M, Alho H, Nikkari ST, Olsson U, Ryderberg U, Sillanaukee P. Serum sialic acid in a random sample of the general population. Clin Chem 1999; 45: 1842-9. [CrossRef]
• 30. Breitenstein A, Stämpfli SF, Camici GG, Akhmedov A, Ha H, Follath F, et al. Amiodarone inhibits arterial thrombus formation and tissue factor translation. Arter Thromb Vasc Biol 2008;28: 2231-8. [CrossRef]
• 31. Khan R.A, Asad Ti, Feroz Z, Ahmed M. In vıvo examinatıon of the anticoagulant effect of the Brassica oleracea methanol extract. Arch Biol Sci Belgrade 2015; 67(2), 631-8. [CrossRef]