Farmasötik Yumuşak Kapsülde α-Tokoferolün Spektrofotometri ile Kantitatif Tayini

Yıl 2020, , 72 - 76, 26.08.2020

Gamze Özgül Artuç

https://doi.org/10.26650/experimed.2020.0009

Öz

Amaç: E vitamini sağlıklı durumun korunması ve hastalıkların önlenmesi için gerekli bir mikro besin maddesidir. Bu çalışmanın amacı, farmasötik preparatlarda α-tokoferolün belirlenmesi için basit, duyarlı ve kolayca uygulanabilir bir spektrofotometrik yöntem geliştirmek ve doğrulamaktır.

Gereç ve Yöntem: Farmasötik preparatlarda α-tokoferolün kantitatif tayini 290 nm’de ölçülen maksimum absorbans değeri kullanılarak gerçekleştirildi. Kalibrasyon grafikleri, beş farklı konsantrasyonda (10-100 µg/mL) standart α-tokoferol örneklerinin absorbanslarına karşılık gelen konsantrasyonların çizilmesiyle oluşturuldu.

Bulgular: Farmasötik yumuşak kapsüldeki α-tokoferol miktarı %101,572 (203,145 IU/kapsül) olarak hesaplandı (Evicap yumuşak kapsül etiket içeriği: 200 IU/kapsül).

Sonuç: Bu çalışmada geliştirilen spektrofotometrik yöntemin doğru, duyarlı, hassas, tekrarlanabilir olduğu ve yumuşak kapsül ve diğer farmasötik preparatlara kolayca uygulanabileceği ileri sürülmektedir.

Anahtar Kelimeler

Spektrofotometri, farmasötik yumuşak kapsül, α-tokoferol

Quantitative Determination of α-Tocopherol in Pharmaceutical Soft Capsule by Spectrophotometry

Öz

Objective: Vitamin E is an essential micronutrient for maintaining a healthy status and preventing disease. The purpose of this study was to develop and validate a simple, sensitive and easily applicable spectrophotometric method for determination of α-tocopherol in pharmaceutical preparations.

Material and Method: The quantitative determination of the α-tocopherol in pharmaceutical prepation was carried out using the maximum absorbance value measured at 290 nm. Calibration graphs were constructed by plotting the absorbance against the corresponding concentration of standart α-tocopherol samples at five different concentrations (10-100 µg/mL).

Results: The amount of α-tocopherol in the pharmaceutical soft capsule was calculated as 101.572% (203.145 IU/capsule) (Evicap soft capsule labelled content: 200 IU/capsule).

Conclusion: It suggested that the developed spectrophotometric method in this study is accurate, sensitive, precise, reproducible and easily applied to soft capsules and the other pharmaceutical preparations.

Anahtar Kelimeler

Spectrophotometry, pharmaceutical soft capsule, α-tocopherol

Kaynakça

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