Hücre Mikroenkapsülasyonunda Manuel ve Kapsülasyon Sisteminin Hücre İzolasyon Tipi ve Aljinat Yüzdesine Bağlı Verimliliğinin Karşılaştırılması

Yıl 2021, Cilt: 11 Sayı: 3, 162 - 169, 08.12.2021

Ömer Faruk Düzenli , Beyza Göncü , Emrah Yücesan , Harika Salepcioğlu , Yeliz Emine Ersoy , Adem Akçakaya

https://doi.org/10.26650/experimed.2021.942737

Öz

Amaç: Birçok endüstriyel sektörde kullanılan polimer malzemeler sağlık bilimlerinde de farklı işlemlerde kullanılmaktadır. Bu işlem-lerden biri olan enkapsülasyon sistemi hücre nakli gibi terapötik uygulamalarda tercih edilmektedir. Enkapsülasyon çalışmalarında uygulanacak yaklaşıma göre kapsül yapısında kullanılacak polimer malzeme ve oluşan kapsül boyutu değişmektedir. Aljinat, kahverengi alglerden elde edilen, içeriğindeki farklı polimerik blok oranlarına bağlı olarak değişiklik gösteren doğal polimerlerden biridir. Bu çalışmada, kapsülasyon aşaması için kullanılacak olan değişik aljinat yüzdeleri uygulanarak paratiroid hücrelerinde ideal mikroenkapsülasyon prosedürlerinin belirlenmesi amaçlanmaktadır.

Gereç ve Yöntem: Çalışmada sekonder hiperparatiroidi hastasın-dan alınan bir adet paratiroid hiperplazi dokusundan mekanik ve enzimatik izolasyon yöntemleriyle hücre eldesi gerçekleştirilmiş-tir. İki farklı aljinat yüzdesi kullanarak hem manuel olarak hem de kapsülasyon cihazında otomatize olarak iki farklı akış hızı değerlendirilmiştir. Mikroenkapsüle edilen hücreler 64-79 gün boyunca in vitro olarak parathormon miktarları ölçülerek takip edilmiştir.

Bulgular: Değerlendirilen aljinat yüzdelerinden %2’lik konsant-rasyona sahip mikroenkapsüllerin oluşturulmasında kapsülasyon cihazında kullanılan 2 mL/dk akış hızıyla morfolojik stabilite gözlenmiştir. Ayrıca parathormon salınımı açısından hücre izolasyon tipi ve aljinat yüzdeleri arasında benzer sonuçlar elde edilmiştir.

Sonuç: Uzun süreli mikroenkapsülasyon verimliliğinin arttırılması için yapısal ve fonksiyonel açıdan birçok parametrenin belirlenmesi gerekmektedir. Bu çalışma ile enzimatik izolasyon metoduyla elde edilen paratiroid hücrelerinin kapsülasyon sistemi kullanılarak artan akış hızında daha stabil bir yapı oluşturdukları belirlenmiştir.

Anahtar Kelimeler

Paratiroid, mikroenkapsülasyon, ultra saf aljinat, kapsülasyon sistemi, hücre izolasyonu

Destekleyen Kurum

Bezmialem Vakıf Üniversitesi

Proje Numarası

20200204

Teşekkür

Ekipman desteği için Bezmialem Vakıf Üniversitesi Tıbbi Biyoloji AD.’dan Prof. Fahri Akbaş’a teşekkür ederiz.

A Comparison of Cell Micro-encapsulation Efficiency of a Manually Produced and Capsulation System by Depending on the Cell Isolation Type and the Alginate Percentage

Öz

Objective: Many industrial sectors in the health sciences are using polymeric materials for different processes. One of these processes is the encapsulation system mainly preferred in therapeutic applications including cell transplantation. Depending on the main approach, the polymer type and sphere properties differ by the encapsulation method. Alginate is one of the natural polymers that show structural changes depending on different polymer ratios. In this study, our aim is to determine the ideal micro-encapsulation procedures by evaluating different alginate concentrations for the capsulation process of parathyroid cells.

Material and Method: In this study, cell isolation of single parathyroid hyperplasia tissue from a patient with secondary hyperparathyroidism was performed by using two methods including mechanical and enzymatic isolation. Two different alginate percentages were used for micro-encapsulation which created manually, and a capsulation system made with two different flow rates. Parathormone levels of micro-encapsulated cells were followed for 64-79 days in vitro.

Results: Morphological stability was observed for 2% alginate concentration with a flow rate of 2 mL/min used group from the micro-encapsulation system. However, parathyroid hormone release showed similar results between cell isolation type and alginate percentages.

Conclusion: Increasing the long-term micro-encapsulation efficiency, certain criteria should be determined from structural and functional aspects. In this study, the microencapsulation of parathyroid cells ob-tained by the enzymatic isolation method formed a more stable structure at increasing flow rate by using the capsulation system.

Anahtar Kelimeler

Parathyroid, micro-encapsulation, ultra-pure alginate, cap-sulation system, cell isolation

Proje Numarası

20200204

Kaynakça

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