Evaluation of the Effect of Ethyl Alcohol Solutions in Different Concentrations on Bacteria

Yıl 2024, , 220 - 229, 26.08.2024

Naile Akıncı , Meriç Çelik , Hakan Yasin Çetinkaya , Gülnaz Çelik

https://doi.org/10.56061/fbujohs.1470944

Öz

Objective: In this randomized controlled experimental study, it is aimed to evaluate the effect of different concentrations of ethyl alcohol solutions on bacterial growth
Material and Methods: Alcohol solutions of different concentrations by volume were prepared as a preliminary process. Microbiological agar was used as the medium. As the third process, sterile Petri dishes were obtained, and the dishes to be used were labelled as dirty hand, ethyl alcohol solutions of 10%, 30%, 60%, 70%, 80%, and 96%.
Results: It was observed that the count of colonies in petri dishes decreased visibly as the alcohol concentration increased. Approximately 74% reduction was observed in bacterial colonies between 10% ethyl alcohol to 30% ethyl alcohol. About 51% reduction was observed in bacterial colonies between 30% ethyl alcohol to 60% ethyl alcohol. As seen in the table, as the ethyl alcohol concentration increased, the count of colonies detected decreased and eventually vanished with 70% ethyl alcohol.
Conclusion: The lowest and most effective ethyl alcohol concentration was found to be 70%. As a result of the research, we think that observing bacterial growth at different alcohol concentrations with samples taken from different environments in future research will provide us with more data on the effect of disinfectants and antiseptics.

Anahtar Kelimeler

Ethanol, hand snitizers, COVID-19, disinfectants

Farklı Konsantrasyonlardaki Etil Alkol Çözeltilerinin Bakteriler Üzerindeki Etkisinin Değerlendirilmesi

Öz

Amaç: Bu randomize kontrollü deneysel araştırmada farklı konsantrasyonlardaki etil alkol çözeltilerinin bakteri oluşumu üzerine etkisinin değerlendirilmesi amaçlanmaktadır.
Yöntem: Ön işlem olarak hacimce farklı konsantrasyonda alkol çözeltileri hazırlanmıştır. Besiyer olarak mikrobiyolojik Agar kullanılmıştır. Üçüncül işlem olarak steril petri kapları alınmış ve kullanılacak petri kapları kirli el,%10 ,%30 , %60 , %70 ,%80 ve %96’lık etil alkol çözeltileri olarak etiketlenmiştir. Günlük hayatta sık kullanılan araçlarından biri olan laptop (monitör ve klavye) örnek alınacak materyal olarak seçilmiştir.
Bulgular: Araştırma sonucunda alkol konsantrasyonu arttıkça petri kaplarının içerisindeki koloni sayılarının göz ile görülür derecede azaldığı gözlemlenmiştir.
Sonuç: En düşük ve etkili etil alkol konsantrasyonu %70 olarak saptanmıştır. İleriki araştırmalarda farklı ortamlardan alınan örneklerle farklı alkol konsantrasyonlarında bakteri üremesinin gözlemlenmesinin dezenfektan ve antiseptiklerin etkisi konusunda daha fazla bilgiye ulaşmamızı sağlayacağı düşünülmektedir.

Anahtar Kelimeler

Etanol, el antiseptikleri, Covıd-19, dezenfektanlar

Kaynakça

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