The Comparison of Sensitivity of Various Methods in The Detection of Olive Tree Viruses

Yıl 2016, Cilt: 45 Sayı: 1, 1 - 13, 30.09.2016

Serpil Erilmez

Öz

To compare
the sensivity of different methods used to detect the agents of viral diseases
in olive trees, totaly 375 samples of leaves, fruits and flowers were collected
from olive trees in Aydın, Balikesir and Izmir provinces and for further
studies, 27 samples were selected from 375 ones as a one representative sample
from each of counties in these provinces. The existence of 9 viral agents in
these samples was checked by RT-PCR while the detection of 4 viruses was done
with DAS-ELISA. When the juice from the samples was inoculated to certain test
plants, they did not produce any symptoms. It was found that all samples were
infected with the any of viruses entitled Arabis mosaic virus (ArMV), Cherry
leafroll virus (CLRV), Strawberry latent ringspot virus (SLRSV) and Cucumis
mosaic virus (CMV) by RT-PCR while ArMV was present in only 5 samples in
DAS-ELISA. Moreover, all samples in this study were free from Olive latent-1
virus (OLV-1), Olive latent-2 virus (OLV-2), Olive latent-3 virus
(OLV-3), Olive latent ringspot virus (OLRSV) and Olive leaf
yellowing-associated virus (OLYaV) by RT-PCR. The results of RT-PCR showed
that the samples from 3 trees having virus-like symptoms were infected with a
virus and on the contrary, 24 samples from the trees which were determined to
be infected with viruses did not demonstrated any virus symptoms. In
conclusion, it was concluded that RT-PCR was the most sensitive method in the
detection of the viruses in olive trees at this moment and has the potential usage
on the applications of quarantine and certification programs in safety.

Anahtar Kelimeler

Olive, Virus

Kaynakça

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