Düzeltme: Effects of 17-α-Methyltestosterone usage on Coloration, Sex Reversal and Reproductive Performance in Guppy (Poecilia reticulata) Fish

Yıl 2023, Cilt: 12 Sayı: 2, 197 - 208, 17.11.2023

Ebru Yılmaz , Muammer Erdem

Bu makalenin ilk hali 30 Haziran 2023 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/gbad/issue/77433/1226882

Düzeltme Notu

Öz

In this study, 17 -methyltestosterone hormone was added to the feed and water, and the effects of coloring, sex reversal and reproduction were investigated in guppy fish (Poecilia reticulata). Experimental groups were planned as control group (K), water hormone (S) and feeding hormone (Y) groups in 3 groups with 3 replicates. The 3 -stage study lasted a total of 12 weeks. In the 1st and 2nd stages of the experiment, visual color determination was performed with 10 panelists using a color scale at weekly intervals. 1 ml of the stock solution containing 0.1 mg MT was added to water and feed, while the control group was not treated with hormone. At the end of the 1st phase of the experiment, the highest mean color scale values were found in the S group (29.90±0.23), followed by the Y (29.10±0.18) and K (20±0.00) groups, and significant differences were determined between the groups (p<0.05). At the end of the 2nd phase of the experiment, the highest mean color scale values were found in the S group (30.83±0.13), followed by the Y (29.60±0.11) and K (20±0.00) groups, and the difference between the groups was found statistically significant (p<0.05). In the experiment, gonopodium formation was observed in S group in the 1st stage in 5 fish and in the 2nd stage in 1 fish in total 6 fish, and in Y group in the 1st and 2nd stages in 1 fish in total 2 fish. In group S, androgen hormone was found to cause more sex reversal. The results of the current study in terms of the effect of hormone on reproduction, while there was no reproduction in the S and Y groups, a total of 18 fry were obtained from 2 fish in the K group. As a result, it was determined that the effect of 17α-MT hormone use on coloration and sex reversal in guppies was positive, while its effect on reproduction was negative.

Anahtar Kelimeler

Sex reversal, Guppy, Poecilia reticulata, Coloration, Reproduction, 17α-methyltestosterone

Düzeltme: 17-α-Metiltestosteron Hormonu Kullanımının Lepistes (Poecilia reticulata) Balıklarında Renklenme, Cinsiyet Dönüşümü ve Üreme Performansı Üzerine Etkisi

Bu makalenin ilk hali 30 Haziran 2023 tarihinde yayımlandı. https://dergipark.org.tr/tr/pub/gbad/issue/77433/1226882

Düzeltme Notu

Bu makale, GBAD, 2023 2023, 12(1), 55-66 sayfaları arasında basılmış olan makalenin düzeltilmiş halidir

Öz

Bu araştırmada, yeme ve suya 17 -metiltestosteron hormonu katılarak lepistes balıklarında (Poecilia reticulata) renklenme, cinsiyet dönüşümü ve üreme üzerine etkileri araştırılmıştır. Deneme grupları; kontrol grubu (K), suya hormon (S) ve yeme hormon (Y) grupları olarak 3 grupta 3 tekerrürlü olacak şekilde planlanmıştır. 3 aşamalı olan çalışma toplam 12 hafta sürmüştür. Denemenin 1. ve 2. aşamalarında haftalık periyotlarda renk skalası kullanılarak 10 panelist ile görsel renk tayini yapılmıştır. 1 ml’sinde 0.1 mg MT bulunan stok çözeltiden suya ve yeme katılmış, kontrol grubuna ise hormon uygulanmamıştır. Denemenin 1. aşaması sonunda renk skalası değerleri ortalaması en yüksek S grubunda (29.90±0.23) tespit edilmiş olup, bunu Y (29.10±0.18) ve K (20±0.00) grupları takip etmiş, gruplar arasında istatistiksel farkın önemli olduğu belirlenmiştir (p<0.05). Denemenin 2. aşaması sonunda renk skalası değerleri ortalaması en yüksek S grubunda (30.83±0.13) tespit edilmiş olup, bunu Y (29.60±0.11) ve K (20±0.00) grupları takip etmiş, gruplar arasında fark istatistiksel açıdan önemli bulunmuştur (p<0.05). Denemede S grubunda 1. aşamada 5 adet, 2. aşamada 1 adet toplam 6 adet balıkta, Y grubunda ise 1. ve 2. aşamalarda 1’er adet, toplam 2 adet balıkta gonopodium oluşumu gözlenmiştir. S grubunda androjen hormonun daha fazla cinsiyet dönüşümüne neden olduğu görülmüştür. Üremeye etkisi bakımından çalışma sonucuna baktığımızda S ve Y gruplarında üreme olmazken K grubunda 2 balıktan toplam 18 yavru elde edilmiştir. Sonuç olarak 17α-MT hormon kullanımının lepistes balıklarında renklenme ve cinsiyet dönüşümü üzerinde etkisinin olumlu olduğu, üreme üzerine etkisinin ise olumsuz olduğu tespit edilmiştir.

Anahtar Kelimeler

Cinsiyet Dönüşümü, Lepistes, Poecilia reticulata, Renklenme, Üreme, 17-α-metiltestosteron, Cinsiyet Dönüşümü, Lepistes, Poecilia reticulata, Renklenme, Üreme, 17-α-metiltestosteron

Kaynakça

• Arslan, T., Güven, E., Baltacı, M.A., 2010. Hormonal cinsiyet dönüşüm metodu kullanarak monoseks gökkuşağı alabalığı (Oncorhynchus mykiss) üretimi. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 16(Suppl-B), 361-368, https://doi.org/10.9775/kvfd.2010.2678.
• Asimi, O.A., 2009. To study the effect of photosensitizer on melanophore responses of blue gourami (Trichogaster trichopterus). Journal of Cell & Animal Biology, 3(5), 083-087.
• Atar, H.H., Bekcan, S., Dogankaya, L., 2009. Effects of different hormones on sex reversal of rainbow trout (Oncorhynchus mykiss Walbaum) and production of all-female populations. Biotechnology & Biotechnological Equipment, 23(4), 1509-1514, https://doi.org/10.2478/V10133-009-0002-X.
• Axelrod, H.R., 1983. Guppies in color. T F.H. Publications, Inc. Ltd., US, 32p.
• Bayley, M., Junge, M., Baatrup, E., 2002. Exposure of juvenile guppies to three antiandrogens causes demasculinization and a reduced sperm count in adult males. Aquatic Toxicology, 56, 227–239, https://doi.org/10.1016/s0166-445x(01)00210-7.
• Biswas, A., Behera, S., Das, P., Meena, D.K., Behera, B.K., 2014. Effect of methyl testosterone (17α-MT) on the phenotype, bioindices and gonads of adult male dwarf gourami (Colisa lalia). Emirates Journal of Food & Agriculture (EJFA), 26(5), 459-464, https://doi.org/10.9755/ejfa.v26i5.16365.
• Das, A.P., Biswas S.P., 2016. Carotenoids and pigmentation in ornamental fish. Journal of Aquaculture & Marine Biology (JAMB), 4(4), 00093, https://doi.org/10.15406/jamb.2016.04.00093.
• Degani, G., Gallagher, M.L., 1985. Effect of dietary 17 -methyltestosterone and bovine growth hormone on growth and food conversion of slow and normal growing american elvers (Anguilla rostrata). Canadian Journal of Fisheries & Aquatic Sciences, 42, 185-189.
• Degani, G., 1986. Effect of combined dietary 17 -estradiol and 17 - methyltestosterone on growth and body composition of european eels (Anguilla anguilla). Aquaculture, 59, 169-175.
• Demir, N., 2006. İhtiyoloji, Nobel Yayın Dağıtım, Yayın No: 924, Fen ve Biyoloji Yayınları Dizisi: 31, 3. Basım, Ankara, Türkiye, 423s.
• Endler, J.A., 1980. Natural selection on color patterns in Poecilia reticulata. Evolution, 34(1), 76-91, https://doi.org/10.2307/2408316.
• Ergün, S., 1997. Doğal ve sentetik karotenoid kaynaklarının gökkuşağı alabalıklarında (Oncorhynchus mykiss) pigmentasyona etkisi. Doktora Tezi, Ondokuz Mayıs Üniversitesi, Fen Bilimleri Enstitüsü, Sinop, 77.
• Evans, J.P., Bisazza, A., Pilastro, A., 2004. Female mating preferences for colourful males in a population of guppies subject to high predation. Journal of Fish Biology, 65, 1154–1159, https://doi.org/10.1111/j.1095-8649.2004.00502.x.
• Fabanjo, M.A., Abdullah, N., 2021. Effect of addition of pro-enzymes and Spirulina flour on growth and color intensity of betta fish (Betta sp). 2nd International Conference on Fisheries and Marine, IOP Conf. Series: Earth and Environmental Science 890, 012025, https://doi.org/10.1088/1755-1315/890/1/012025.
• Foss, P., Storebakken, T., Schiedt, K., Liaaen-Jensen, S., Austreng, E. Streiff, K., 1984. Carotenoids in diets for salmonids. 1. pigmentation of rainbow trout with the ındividual optical ısomers of astaxanthin in comparison with canthaxanthin. Aquaculture, 41, 213-226.
• Goodrich, H.B., Dee, J.E., Flynn, C.M., Mercer, R.N., 1934. Germ cells and sex differentiation in Lebistes reticulatus. Biological Bulletin, 67(1), 83-96.
• Goodwin, T.W., 1951. Carotenoids in fish. In: The biochemistry of fish. Biochemical Society Symposia, USA.
• Guerrero, R.D., 1975. Use of androgens for the production of all male (Tilapia aurea). Transactions of the American Fisheries Society, 104(2), 342-348.
• Hata, M., Hata, M., 1973. Studies on astaxanthin formation is some freshwater fishes. Tohoku Journal of Agricultural Research, 24(4), 192-196.
• Hopper, A.F., 1965. Inhibition of Regeneration of the gonopodium of the guppy by treatment with thiouracil. The Journal of Experimental Zoology, 159, 231-240.
• Houde, A.E., 1997. Sex, color, and mate choice in guppies. Princeton University Press. Princeton, NJ., 210p.
• Jenkins, R.J., Angus, R.A., McNatt, H., Howell, W.M., Kemppainen, J.A., Kirk, M., Wilson, E.M., 2001. Identification of androstenedione in a river containing paper mill effluent. Environmental Toxicology & Chemistry, 20, 1325-1331, https://doi.org/10.1897/1551-5028(2001)020<1325:ioaiar>2.0.co;2.
• Jessy, D., Varghese, T.J., 1988. Hormonal sex control in Betta splendens (Regan) and Xiphophorus helleri (Heckel). Paper presented to First Indian Fisheries Forum, Asian Fisheries Society, Indian Branch, Mangalore, India, pp 26-31.
• Johnson, G., Wathne, E., 1989. Color measurements in farmed salmon and trout. (Translated and Reprinted Form: Norsk Fiskeoppdrett, 4, 45-47). Roche Print. Animal Nutritional and Health, Basel, Switzerland.
• Karslı, Z., Aral, O., Yeşilayer N., 2016. The effects of different proportions of the 17β-estradiol and 17α-methyltestosterone on growth, sex reversal and skin colouration of the electric blue hap (Sciaenochromis ahli Trewavas, 1935). Aquaculture Research, 47, 640–648, https://doi.org/10.1111/are.12524.
• Karslı, Z., Şahin, D., Öz, M., Aral, O., 2018. The effect of hormone (17α-methyltestosterone, 17β-estradiol) usage on development, sex inversion and pigmentation of electric yellow cichlid (Labidochromis caeruleus Fryer, 1956). Applied Ecology and Environmental Research, 16(6), 8093-8103, https://doi.org/10.15666/aeer/1606_80938103.
• Karslı, Z., 2021. Effects of synthetic androgen (17α-methyltestosterone) and estrogen (17β-estradiol) on growth and skin coloration in emperor red cichlid, Aulonocara nyassae (Actinopterygii: Cichliformes: Cichlidae). Acta Ichthyologica et Piscatoria, 51(4), 357–363, https://doi.org/10.3897/aiep.51.70223.
• Karslı, Z., Şahin, D., Öz, M., Öz, Ü., Aral, O., 2022. The effect of dietary supplementation of 17α-methyltestosterone and 17β-estradiol hormones on growth, sex conversion and reproduction in electric yellow cichlid (Labidochromis caeruleus). Pakistan Journal of Zoology, 54(1), 213-218, https://dx.doi.org/10.17582/journal.pjz/20210105110138.
• Kayım, M.H., 1997. 17 -metiltestosteronun kılıçkuyruk (Xiphophorus helleri H. 1848) balığında büyüme üzerine etkisi. Yüksek Lisans Tezi, Ege Üniversitesi, Fen Bilimleri Enstitüsü, İzmir, 150.
• Kodric-Brown, A. (1985). Female preference and sexual selection for male coloration in the guppy (Poecilia reticulata). Behavioral Ecology and Sociobiology, 17, 199-205.
• Larsson, D.G.J., Kinnberg, K., Sturve, J., Stephensen, E., Skön, M., Förlin, L., 2002. Studies of masculinization, detoxification and oxidative stres responses in guppies (Poecilia reticulata) exposed to effluent from a pulp mill. Ecotoxicology and Environmental Safety, 52, 13-20, https://doi.org/10.1006/eesa.2001.2139.
• Lim, L.C., Chao, T.M., Khoo, L.T., 1990. Observations on the breeding of brown- marbled grouper, Epinephelus fuscoguttatus (Forskal). Singapore journal of Primary Industries, 18, 66-84.
• Matty, A.J., 1985. Fish endocrinology. Timber Press Ltd. Portland, USA, 272p.
• Nagy, A., Bercsenyi, M. Csanyi, V., 1981. Sex reversal in carp (Cyprinus carpio) by oral administration of methyltestosterone. Canadian Journal of Fisheries & Aquatic Sciences, 38, 725- 728.
• Octaviani, D.A., Armando, E., Jaelani, A.Q. 2022. Effect of carrot flour (Daucus carota) to increase growth and color brightness in lemon cichlid fish (Labidochromis caeruleus). Asian Journal of Aquatic Sciences, 5(2), 264-270.
• Owusu-Frimpong, M., Nijjhar, B., 1981. Induced sex reversal in Tilapia nilotica (Cichlidae) with methyl testosterone. Hydrobiologia, 78, I57-160.
• Pandey, S., 1969. Effects of hypophysectomy on the testis and secondary sex characters of the adult guppy Poecilia reticulata Peters. Canadian Journal of Zoology, 47, 775-781.
• Pawlowski, S., Sauer, A., Shears, J.A., Tyler, C.R., Braunbeck, T., 2004. Androgenic and estrogenic effects of the synthetic androgen 17-methyltestosterone on sexual development and reproductive performance in the fathead minnow (Pimephales promelas) determined using the gonadal recrudescence assay. Aquatic Toxicology, 68(3), 277–291, https://doi.org/10.1016/j.aquatox.2004.03.018.
• Perera, G.S.C., Bhujel, R.C., 2022. Replacement of fishmeal by house cricket (Acheta domesticus) and field cricket (Gryllus bimaculatus) meals: Effect for growth, pigmentation, and breeding performances of guppy (Poecilia reticulata). Aquaculture Reports, 25, 101260, https://doi.org/10.1016/j.aqrep.2022.101260.
• Riley, L.G., Richan III. N.H., Hirano, T., Grau, E.G., 2002. Activation of the growth hormone ınsulin-like growth factor axis by treatment with 17- methyltestosterone and seawater rearing in the tilapia, Oreochromis mossambicus. General and Comparative Endocrinology, 127, 285-292, https://doi.org/10.1016/s0016-6480(02)00051-5.
• Sezgi, H.B., Bekcan, S., 2008. Farklı periyotlarda 17-α-metiltestosteron ile beslemenin tilapya balıklarının (Oreochromis niloticus L.) cinsiyet dönüşümü üzerine etkileri. Tarım Bilimleri Dergisi, 14(3), 222-229, https://doi.org/10.1501/Tarimbil_0000001034.
• Sathyaruban, S., Uluwaduge, D.I., Yohi, S., Kuganathan, S. 2021. Potential natural carotenoid sources for the colouration of ornamental fish: a review. Aquaculture International, 29, 1507–1528. https://doi.org/10.1007/s10499-021-00689-3.
• Shahidian, M., Jamili, S., Rezaiee, F., Amri, N., 2014. Investigating the effect of 17α-methyl testosterone hormone on hue and color measurement of Scatophagus argus. Marine Science, 4(1), 21-25, https://doi.org/10.5923/j.ms.20140401.03.
• Smith, H.T., 1976. Various aspects of reproductive control in freswater fishes. Master Thesis, Virginia Polytechnic Institude and State University, Blacksburg, VA, 76p.
• Smith, B.E., Hardy, R.W., Torrissen, O.J., 1992. Synthetic astaxanthin deposition in pan-size coho salmon (Oncorhynchus kisutch). Aquaculture, 104(1-2), 105-119, https://doi.org/10.1016/0044-8486(92)90141-7.
• Turan, F., 2001. Farklı düzeylerde suya ve yeme katılan androjen hormonunun (17-methyltestosterone) lepistes balıklarında (Poecilia reticulata) cinsiyet dönüşümü ve büyüme üzerine etkileri. Yüksek Lisans Tezi, Mustafa Kemal Üniversitesi, Fen Bilimleri Enstitüsü, Antakya, 69.
• Widyawati, Y., Fakhri, M., Dailami, M., Arifin, N.B., 2021. Masculinization of Nile tilapia (Oreochromis niloticus) using 17α-methyltestosteron. Tomini Journal of Aquatic Science, 2(1), 9–13.
• Withers, P.C., 1992. Comparative Animal Physiology. Brooks Cole-Tomson Learning. Saunders College Publishing/harcourt Brace Jovanovich College, USA, 949p.
• Yamamoto, T., 1958. Artificial induction of functional sex-reversal in genotypic females of the medaka (Oryzias latipes). The Journal of Experimental Zoology, 137, 227-263.