Laktitolün Genotoksik ve Sitotoksik Etkilerinin İncelenmesi

Yıl 2022, Cilt: 3 Sayı: 2, 133 - 143, 25.11.2022

Ece Avuloğlu Yılmaz , Sevcan Mamur , Esra Erikel , Deniz Yüzbaşıoğlu , Fatma Ünal

Öz

Laktitol bir poliol (şeker alkolü) olup tatlandırıcı olarak kullanılan bir gıda katkı maddesidir. Tatlılar, sakızlar, hamur işleri ve ekmekler gibi sık tüketilen gıdalarda bulunmakla birlikte bazı farmasötiklerde de bulunmaktadır. Kullanımları sürekli artan gıda katkı maddelerinin sağlığa olan etkileri de çokça tartışılmaktadır. Genotoksisite, biyolojik, fiziksel veya kimyasal herhangi bir ajanın DNA hasarına neden olabilme potansiyeli olarak tanımlanır ve söz konusu hasarın mutasyona neden olabileceği kabul edilmektedir. Sitotoksisite testleri, maddelerin çeşitli dokulara ya da hücrelere toksisitesini belirlemek için kullanılır. Bu çalışmada, laktitolün genotoksik ve sitotoksik etkisini değerlendirmek amacıyla comet ve MTT testleri kullanılmıştır. Comet testi sağlıklı iki donörden alınan kan örneklerinden izole edilen lenfositlerde, MTT testi ise BHK-21 An 31 yavru hamster böbrek hücrelerinde gerçekleştirilmiştir. Hücrelere laktitolün (125 µg/mL sadece MTT testinde), 250, 500, 1000, 2000 ve 4000 µg/mL’lik konsantrasyonlarıyla comet testinde 1 saat, MTT testinde 24 saat muamele edilmiştir. Sonuçta laktitolün önemli bir genotoksik ve sitotoksik etki göstermediği tespit edilmiştir. Ancak bu sonuçların farklı test yöntemleri ve hücre grupları ile desteklenmesi gereklidir.

Anahtar Kelimeler

Laktitol, Genotoksisite, Sitotoksisite, Comet, MTT

Teşekkür

BHK-21 An 31 yavru hamster böbrek hücrelerinin temini için Dr. Şükran Yılmaz’a teşekkürlerimi sunarım.

Kaynakça

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