Effects of Different Culture Media Compositions on In Vitro Micropropagation from Paradox Walnut Rootstock Nodes

Yıl 2022, Cilt: 9 Sayı: 4, 500 - 515, 31.12.2022

Cem Dirlik , Hacer Kandemir , Nurberat Çetin , Senem Şen , Begüm Güler , Aynur Gürel

https://doi.org/10.54287/gujsa.1194822

Cited By: 3

Öz

The aim of this study is to establish an effective protocol for in vitro micropropagation from node explants of the 'Paradox' (Juglans regia x J. hindsii) rootstock. In the first stage of the study, the node explants were cultured in semi-solid media after being exposed to different sterilization methods. The most effective sterilization method was observed as ST2 with the lowest darkening rate (4 %). According to the results, SP4 medium provided the highest number of shoots per unit explant (1.05 shoots/explant) and 100 % shoot formation, SP2 medium provided the best mean shoot length (1.6 cm) and the mean number of nodes per unit explant (7.95 nodes/explant). Best root induction was provided from ½ Modified MS medium containing 4 mg/L IBA with 30 g/L sucrose. For root induction explants were retained under dark conditions for 7 days at 24±2oC. In order to ensure root formation and elongation, shoot explants with root induction cultured in a 16-hour photoperiod (3500 lux) for 21 days in different nutrient media containing 2.4 mg/L Gelrite, hormone-free and mixed with vermiculite (1:1 v/v) and was rooting rate is 45.45 % in ¼DFe medium.

Anahtar Kelimeler

Micropropagation, Walnut, Juglans regia L., Paradox, In vitro

Kaynakça

• Açıksöz, U. (2020). Bandırma İlçesindeki Ceviz Fidanı Üretiminin Mevcut Durumunun İncelenmesi. MSc Thesis, Bursa Uludağ University.
• Aslansoy, B. (2012). Sultandağı (Afyon) yöresi cevizlerinin (Juglans regia L.) seleksiyon yoluyla ıslahı üzerine araştırmalar. . MSc Thesis, Selçuk University.
• Bosela, M. J., & Michler, C. H. (2008). Media effects on black walnut (Juglans nigra L.) shoot culture growth in vitro: Evaluation of multiple nutrient formulations and cytokinin types. In Vitro Cellular and Developmental Biology - Plant, 44(4), 316-329. doi:10.1007/s11627-008-9114-5
• Budak, Y. (2010). Ceviz Yetiştiriciliği. Samsun İl Tarım Müdürlüğü, Çiftçi Eğitimi ve Yayım Şubesi Yayınları, 20 p., Samsun. PDF
• Caboni, E., & Damiano, C. (2006). In vitro propagation of walnut (Juglans regia L.): Critical factors for the induction of the rooting response. Acta Horticulturae, 705, 329-333. doi:10.17660/ActaHortic.2005.705.44
• Caboni, E., & Lauri, P. (1995). Effetto di fattori chimici, fisici e trasformazione localizzata sulla radicazione in vitro di noce. Italus Hortus, 4(2), 49-53.
• Dong, P., Lichai, Y., Qingming, W., & Ruisheng, G. (2007). Factors affecting rooting of in vitro shoots of walnut cultivars. The Journal of Horticultural Science and Biotechnology, 82(2), 223-226. doi:10.1080/14620316.2007.11512223
• Driver, J. A., & Kuniyuki, A. H. (1984). In vitro propagation of Paradox Walnut Rootstock. Hortscience, 19(4). 507-509.
• Er, E., Akyüz, B., Öztürk, A., & Serdar, Ü. (2017). Cevizde Çeşit Değiştirme: Kabukaltı Aşısı. Bahçe, 46(Special Issue-2), 307-312.
• Fidancı, A. (2005). Şebin ve KR-2 ceviz çeşitlerinin in vitro’da hızlı çoğaltılma tekniklerinin belirlenmesi. Bahçe, 34(1), 239-246.
• Fu, Y., Gu, F., & Wu, W. (2003). Sterilization of Carya illinoensis Explants in Tissue Culture. Journal of Anhui Agricultural University, 31(2), 169-172.
• Gürel, A., Hayta, Ş., Nartop, P., Bayraktar, M., & Fedakar S. O. (2013). Bitki Hücre, Doku ve Organ Kültürü Uygulamaları. Ege Üniversitesi Yayınları Yayın No: 58.
• Jay-Allemand, C., Capelli, P., & Cornu, D. (1992). Root development of in vitro hybrid walnut microcuttings in a vermiculite-containing gelrite medium. Scientia Horticulturae, 51(3-4), 335-342. doi:10.1016/0304-4238(92)90132-V
• Kamali, K., Majidi, E., & Zarghami, R. (2001). Micropropagation of GF-677 Rootstocks (Prunus amygdalus x P. persica). Seed and Plant, 17, 175-177.
• Kepenek, K., & Kolağası, Z. (2016). Micropropagation of Walnut (Juglans regia L.). Acta Physica Polonica A, 130(1), 150-156. doi:10.12693/APhysPolA.130.150
• Kumar, P. P., & Loh, C. S. (2012). Plant Tissue Culture for Biotechnology. In: A. Altman, & P. M. Hasegawa (Eds.), Plant Biotechnology and Agriculture-Prospect for the 21st Century (pp. 131-138) Academic Press. doi:10.1016/B978-0-12-381466-1.00009-2
• Kyte, L. (1987). Plants from Test Tubes. An Introduction to Micropropagation. Timber.
• Leal, D. R., Sánchez-Olate, M., Avilés, F., Materan, M. E., Uribe, M., Hasbún, R., & Rodríguez, R. (2007). Micropropagation of Juglans regia L. In: S. M. Jain, & H. Häggman (Eds.), Protocols for Micropropagation of Woody Trees and Fruits (pp. 381-390). Springer, Dordrecht. doi:10.1007/978-1-4020-6352-7_35
• Leelavathy, S., & Sankar, P. D. (2016). Curbing the menace of contamination in plant tissue culture. Journal of Pure and Applied Microbiology, 10(3), 2145-2152.
• Licea-Moreno, R., Fira, A., & Chocov, G. (2020). Micropropagation of valuable walnut genotypes for timber production: new advances and insights. Annals of Silvicultural Research, 44(1), 5-13. doi:10.12899/asr-1932
• Lone, I. A. (2017). Effect of different growth regulator combinations on in vitro callusing of walnut (J. regia L.). An Asian Journal of Soil Science, 12(1), 203-209. doi:10.15740/HAS/AJSS/12.1/203-209
• Meier-Dinkel, A., & Wenzlitschke, I. (2015). Micropropagation of mature Juglans hybrids. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 85-92). doi:10.17660/ActaHortic.2017.1155.11
• Murashige, G., & Skoog, F. (1962). A Revised Medium for Rapid Growthand Bioassays With Tobacco Tissue Culture. Physiologia Plantarum, 15(3), 473-497. doi:10.1111/j.1399-3054.1962.tb08052.x
• Ray, S. S., & Ali, N. (2016). Biotic Contamination and Possible Ways of Sterilization: A Review with Reference to Bamboo Micropropagation. Brazilian Archives of Biology and Technology, 59(e16160485). doi:10.1590/1678-4324-2016160485
• Ripetti, V., Kevers, C. & Gaspar, T. (1994). Two successive media for the rooting of walnut shoots in vitro. Changes in peroxidase activity and in ethylene production. Advances in Horticultural Science, 8(1), 29-32.
• Revilla, M. A., Majada, J., & Rodriguez, R. (1989). Walnut (Juglans regia L.) micropropagation. Annales des sciences forestières, INRA/EDP Sciences, 46(Suppl), 149s-151s. PDF
• Rugini, E. (1984). In Vitro Propagation of Some Olive (Olea europaea sativa L.) Cultivars with Different Root-Ability and Medium Development Using Analytical Data from Developing Shoots and Embryos. Scientia Hotriculturae, 24(2), 123-134. doi:10.1016/0304-4238(84)90143-2
• Saadat, Y. A., & Hennerty, M. J. (2002). Factors Affecting the Shoot Multiplication of Persian Walnut (Juglans regia L.). Scientia Horticulturae, 95(3), 251-260. doi:10.1016/S0304-4238(02)00003-1
• Scaltsoyiannes, A., Tsoulpha, P., Panetsos, K. P., & Moulalis, D. (1997). Effect of Genotype on Micropropagation of Walnut Trees (Juglans regia). Silvae Genetica, 46(6), 326-332.
• Schader, M. (2019). The Definition of Necrosis in Plants (Accessed: 25/11/2019) URL (https://sciencing.com/the-definition-of-necrosis-in-plants-12003546.html)
• Stevens, M. E., & Pijut, P. M. (2018). Rapid in vitro shoot multiplication of the recalcitrant species Juglans nigra L. In Vitro Cellular & Developmental Biology-Plant, 54(3), 309-317. doi:10.1007/s11627-018-9892-3
• Şen, S. M. (2011). Ceviz Yetiştiriciliği-Besin Değeri-Folklorü. ÜÇM yayıncılık, Samsun.
• Şirin, E. (2014). Kaman 1 ve Kaman 5 ceviz çeşitlerinin (Juglans regia L.) mikroçoğaltımı. MSc Thesis, Gaziosmanpaşa University.
• Tarinejad, A. (2013). Effects of disinfectants and antibiotics on contamination during propagation of walnut (Juglans regia L.). Research on Crops, 14(1), 219-225.
• Tekielska, D., Peňázová, E., Kovács, T., Křižan, B., Čechová, J., & Eichmeier, A. (2019). Bacterial contamination of plant in vitro cultures in commercial production detected by high-throughput amplicon sequencing. Acta Universitatis Agriculturae et Silviculturae Mendelianae Brunensis, 67(4), 1005-1014. doi:10.11118/actaun201967041005
• Tetsumura, T., Tsukuda, K., & Kawase, K. (2002). Micropropagation of Shinano Walnut (Juglans regia L.). Journal of the Japanese Society for Horticultural Science, 71(5), 661-663. doi:10.2503/jjshs.71.661
• Tuan, P. N., Meier-Dinkel, A., Höltken, A. M., Wenzlitschke, I. & Winkelmann, T. (2017). Factors affecting shoot multiplication and rooting of walnut (Juglans regia L.) in vitro. In: M. Beruto, & E. A. Ozudogru (Eds.), Proceedings of the VI International Symposium on Production and Establishment of Micropropagated Plants, ISHS Acta Horticulturae 1155, (pp. 525-530). doi:10.17660/ActaHortic.2017.1155.77
• Vahdati, K., Ashrafi, E. N., Ebrahimzadeh, H., & Mirmasoumi, M. (2009). Improved micropropagation of walnut (Juglans regia L.) on media optimized for growth based upon mineral content of walnut seed. In: M.-V. Hanke, F. Dunemann, & H. Flachowsky (Eds.), Proceedings of the I International Symposium on Biotechnology of Fruit Species: BIOTECHFRUIT2008, ISHS Acta Horticulturae 839, (pp. 117-124). doi:10.17660/ActaHortic.2009.839.13
• Varghese, N., & Joy, P. P. (2016). Plant tissue culture contaminants identification and its response to fumigation. Technical Report, 1-10.
• Yiğit, D., Yiğit, N., Aktaş, E., & Özgen, U. (2009). Ceviz (Juglans regia L.)’in antimikrobiyal aktivitesi. Türk Mikrobiyoloji Cemiyeti Dergisi, 39(1-2), 7-11.