Elektropolimerize 3,5-Diamino-1,2,4-Triazol Film ile Modifiye Edilmiş Altın Elektrot Yüzeyinde Epinefrinin Voltametrik Tayini ve Elektrokimyasal Davranışı

Yıl 2019, Cilt: 7 Sayı: 4, 985 - 998, 24.12.2019

Tuğba Tabanlıgil Calam

https://doi.org/10.29109/gujsc.623660

Cited By: 2

Öz

Bu
çalışmada, altın elektrot (Au) yüzeyi 3,5-diamino-1,2,4-triazol (35DT) molekülü
ile elektrokimyasal yöntemle modifiye edilmiştir. Epinefrinin (EP) modifiye
elektrot (35DT-Au) yüzeyindeki elektrokimyasal davranışı diferansiyel puls
voltametrisi (DPV) ve dönüşümlü voltametri (CV) teknikleriyle incelenmiştir.
EP'nin yükseltgenme işleminde 35DT-Au modifiye elektrot yüzeyi iyi bir
elektrokatalitik aktivite göstermiştir. EP tayini, 35DT-Au modifiye elektrot
yüzeyinde DPV tekniği kullanılarak gerçekleştirilmiştir. Optimum şartlar
altında, 35DT-Au modifiye elektrot ile EP’ye ait doğrusal çalışma aralığı 0,9-32,31
μM ve 32,31-1050 μM olmak üzere iki farklı derişim aralığı olarak belirlenmiştir.
Bu iki derişim aralığı için gözlenebilme sınırları (LOD) sırasıyla 0,39 ve 12,33
μM olarak bulunmuştur. EP tayini için, 35DT-Au elektrot iyi bir
tekrarlanabilirlik, kararlılık ve hassasiyet göstermiştir. Ayrıca, gerçek
numune olarak ilaç numunei seçilmiş ve ilaçta EP tayini 35DT-Au elektrot ile başarıyla
gerçekleştirilmiştir.

Anahtar Kelimeler

Epinefrin, Kaplama, Elektrokimyasal Davranış, Voltametrik tayin, ilaç

Destekleyen Kurum

Gazi Üniversitesi BAP

Proje Numarası

65/2019‐02

The Voltammetric Determination of Epinephrine on an Au Electrode Modified with Electropolymerized 3,5-Diamino-1,2,4- Triazole Film

Öz

A gold electrode (Au) was modified with 3,5-diamino-1,2,4-triazole (35DT). The electrochemical behavior of epinephrine (EP) was evaluated on the modified electrode (35DT-Au) by the differential pulse voltammetry (DPV) and cyclic voltammetry (CV) techniques. For the oxidation of EP, the 35DT-Au modified electrode surface showed an good electrocatalytic activity. The modified electrode was used to determine EP by using DPV technique. Under the optimum conditions, the calibration curve for EP was obtained in the ranges of 0.9 to 32.31 μM and 32.31 to 1050 μM and the detection limits (LOD) was found as 0.39 and 12.33 μM, respectively. For the oxidation process of EP, the 35DT-Au modified electrode exhibited a good repeatability, reproducibility, stability and sensitivity. Finally, the determination of EP in commercial injectable adrenaline samples were successfully applied.

Anahtar Kelimeler

Epinephrine, Coating, Electrochemical, behaviour, Voltammetric, determination, Drug

Proje Numarası

65/2019‐02

Kaynakça

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