Gümüşhane İlinde Yayılış Gösteren Bazı Tıbbi Bitkilerin Antioksidan ve Antimikrobiyal Aktivitelerinin ve RP-HPLC-DAD ile Fenolik Bileşenlerinin Belirlenmesi

Yıl 2019, Cilt: 9 Sayı: 2, 362 - 372, 15.04.2019

Kaan Kaltalıoğlu , Mustafa Karaköse , Hüseyin Şahin , Ersan Bektaş , Kadriye İnan Bektaş

https://doi.org/10.17714/gumusfenbil.370484

Cited By: 1

Öz

Bu çalışmada, çeşitli
rahatsızlıkların tedavisinde tıbbi amaç için kullanılan ve Gümüşhane ilinden
toplanan Hypericum scabrum, Laser trilobum, Turanecio hypochionaeus ve
Lactuca racemosa bitkilerinin antioksidan ve antimikrobiyal özelliklerinin
analizi ve fenolik bileşenlerinin belirlenmesi amaçlanmıştır. Fenolik
bileşenler diod tarama dedektörlü ters fazlı yüksek performanslı sıvı
kromatografisi (RP-HPLC-DAD) analizi ile belirlenmiştir. Bitki özütlerinde
belirlenen ana bileşenler, klorojenik ve kafeik asit olurken; miktarları ise
sırasıyla 14.08-0.90 ve 12.76-0.35 mg fenolik/g özüt aralığında tespit
edilmiştir. Antioksidan aktivite profiline bakıldığında; demir indirgeme
antioksidan gücünün (FRAP) 378.04-54.10 µM FeSO₄.7H₂O
eşdeğeri/g özüt aralığında, DPPH radikalini %50 oranında süpürmek için gereken
konsantrasyonun 483.33-45.64 µg/mL aralığında, total fenolik içeriklerinin
(TPC) 82.76-36.30 mg GAE/g özüt aralığında olduğu belirlenmiştir.
Antimikrobiyal analiz sonuçlarında ise test edilen mikroorganizmalara karşı
bitki özütlerinin düşük seviyede etki gösterdikleri belirlenmiştir. Edinilen
sonuçlara göre, bu bitkiler yeni farmasötik ilaçların geliştirilmesine katkıda
bulunabilirken; çeşitli hastalıkların tedavisindeki potansiyel kullanımları doz
bağımlı bir şekilde klinik çalışmalarda araştırılabilir kanaati ortaya
çıkmıştır.

Anahtar Kelimeler

Antimikrobiyal aktivite, Antioksidan aktivite, Fenolik, Gümüşhane, Tıbbi bitkiler

Determination of Antioxidant and Antimicrobial Activities, and Phenolics Compounds by RP-HPLC-DAD of Some Medicinal Plants from Gümüşhane (Turkey)

Öz

This study was designed to determine antioxidant and antimicrobial
properties and phenolic constitutes of Hypericum scabrum, Laser trilobum,
Turanecio hypochionaeus and Lactuca racemosa which are collected in Gümüşhane
(Turkey) and are used as a traditional complementary therapy for various
disorders. RP-HPLC-DAD (reverse phase-high performance liquid chromatography
with a diode array detector) was applied for quantitative and qualitative
analysis of phenolic compounds. Chlorogenic acid and caffeic acid were found in
all samples as the dominant compound ranged from 14.08 to 0.90 and 12.76 to
0.35 mg phenolic/g extract, respectively. The antioxidant activity profile was,
ferric reducing antioxidant power (FRAP) ranged from 378.04 to 54.10 µM FeSO₄.7H₂O/g
extract, the concentration desired for scavenging of DPPH radicals by 50%
ranged from 483.33 to 45.64 µg/mL, while total phenolic content (TPC) ranged
from 82.76 to 36.30 mg GAE/g extract. According to antimicrobial results, plant
extracts showed a weak effect against tested microorganisms. In conclusion,
their potential use in treating various diseases in a dose-dependent manner
might also be explored in clinical trials and obtained results might contribute
to the development of new pharmaceutical drugs.

 

Anahtar Kelimeler

Antimicrobial activity, Antioxidant activity, Gümüşhane, Medicinal plants, Phenolics

Kaynakça

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