Rhodobacter sphaeroides O.U.001'den Biyoteknolojik Olarak Üretilen Karotenoidlerin Antioksidan ve Sitotoksik Etkilerinin Araştırılması

Yıl 2020, Cilt: 10 Sayı: 3, 559 - 568, 15.07.2020

Gökhan Kars , Meltem Demirel Kars , İhsan Obalı , Ayça Emsen Ufuk Gündüz

https://doi.org/10.17714/gumusfenbil.597048

Cited By: 2

Öz

Karotenoidler güçlü antioksidan özelliklerinden dolayı dikkat
çekmektedirler. Bu çalışmada, Rhodobacter sphaeroides O.U. 001'den ekstrakte
edilen karotenoidlerin antioksidan ve antiproliferatif etkileri araştırıldı.
Bunun için, R. sphaeroides O.U. 001 önce 5 L'lik bir biyoreaktörde çoğaltıldı.
Daha sonra, çözücü olarak aseton kullanılarak R. sphaeroides O.U.001'den
karotenoidler ekstrakte edildi. Karotenoid ekstraktının, vinkristin ve paklitaksele
dirençli hücrelerin (MCF-7/Vinc, MCF-7/Pac) proliferasyonuna etkileri MTT sitotoksisite
testi ile araştırıldı. Karotenoid ekstraktı ve kanser ilaçları arasındaki
etkileşimin hücreler üzerindeki etkisi dama tahtası plaka tekniği ile
değerlendirildi. Karotenoid ekstraktın antioksidan aktivitesi ise 2, 2 -
difenil, 1-pikril hidrazil (DPPH) radikal süpürücü aktivite tayin yöntemi ile
belirlendi. Ekstraksiyon sonucunda, önemli miktarda karotenoid verimi elde
edildi (80.69 mg karotenoid/g kuru hücre ağırlığı). Karotenoid ekstraktının
hücre proliferasyonu üzerinde inhibitör etkisinin olmadığı ve antikanser
ilaçlarla etkileşiminin antagonistik olduğu bulundu. Bunlara ek olarak, elde
edilen karotenoid ekstraktının DPPH antioksidan etkisi, (IC₅₀ değeri)
25 µg/mL olarak hesaplandı. Sonuç olarak, biyoteknolojik yolla üretilen bakteriyel
karotenoidlerin, kanser hücrelerinde sitotoksik etki göstermeden önemli
antioksidan aktiviteye sahip oldukları gösterilmiştir.

Anahtar Kelimeler

Antioksidan, Biyoreaktör, Karotenoid, MCF-7, Rhodobacter sphaeroides

Destekleyen Kurum

TÜBİTAK

Proje Numarası

110T552

Teşekkür

We would like to thank Prof. Dr. Samuel Kaplan (The University of Texas Health Science Center at Houston) for giving permission to use carotenoid images.

Investigation of Antioxidant and Cytotoxic Effects of Biotechnologically Produced Carotenoids from Rhodobacter sphaeroides O.U. 001

Öz

Carotenoids take special attention due to their
strong antioxidant properties. In the present study, the antioxidant and
antiproliferative effects of carotenoids extracted from Rhodobacter sphaeroides O.U. 001 were investigated. For this, R. sphaeroides O.U. 001 was first cultivated in a 5 L
bioreactor. After that, the carotenoids were extracted from R. sphaeroides O.U. 001 using acetone as solvent. The
effects of carotenoid extract on proliferation of vincristine and paclitaxel
resistant cells (MCF-7/Vinc, MCF-7/Pac) were investigated by MTT cytotoxicity
assay. The checkerboard plate technique was performed to evaluate the
interactions between carotenoid extract and cancer drugs on the cells. The
antioxidant activity of the carotenoid extract was assessed by 2, 2 - diphenyl,
1- picryl hydrazyl (DPPH) radical scavenging assay. As a result of extraction,
considerable amount of carotenoid yield was obtained (80.69 mg carotenoid/g dry
cell weight). It was found that carotenoid extract had no inhibitory effects on
cell proliferation and interaction of carotenoids with anticancer drugs
exhibited antagonistic effects. In addition to these, the DPPH antioxidant
activity (IC₅₀ value) of the carotenoid extract was calculated as 25
µg/mL. In conclusion, biotechnologically produced bacterial carotenoids were
shown to have considerable antioxidant activity without any cytotoxicity on
cancer cells.

Anahtar Kelimeler

Antioxidant, Bioreactor, Carotenoid, MCF-7, Rhodobacter sphaeroides

Proje Numarası

110T552

Kaynakça

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