Kırmızı hevhulma (Lythrum salicaria l.) bitkisinin toplam fenolik bileşik tayini ile antioksidan ve antimikrobiyel aktivitelerinin belirlenmesi

Yıl 2021, Cilt: 11 Sayı: 1, 219 - 228, 15.01.2021

Selin Kalkan , Mehmet Soner Engin , Mustafa Otağ

https://doi.org/10.17714/gumusfenbil.705634

Cited By: 1

Öz

Kırmızı (Tıbbi) hevhulma olarak bilinen Lythrum salicaria L. ishal, kronik bağırsak nezlesi, diş eti kanamaları, hemoroid ve egzama tedavisinde kullanılmaktadır. Bu çalışmada, Lythrum salicaria L.'nin (Lythraceae) kurutulmuş çiçek-yaprak ve sap kısımları, sırasıyla etil alkol (%80 v/v) ve asetik asit-su (1.5:98.5 v/v) gibi farklı çözücüler ile ekstrakte edilmiştir. Tüm ekstraktların toplam fenolik madde miktarları tespit edilerek antimikrobiyel ve antioksidan aktiviteleri in vitro yöntemler kullanılarak araştırılmıştır. Sonuç olarak, toplam fenolik madde miktarını belirlemede uygulanan Folin-Ciocalteu yöntemi sonucunda, en yüksek toplam fenolik bileşik miktarı kırmızı hevhulmanın çiçek-yaprak kısmınınasetik asit-su ekstraksiyonunda olduğu saptanmış ve 5.916 ±0.335 mg Gallik Asit Eşdeğeri (GAE)/g olduğu tespit edilmiştir. Serbest radikal süpürücü aktivite (1,1-difenil-2-pikrilhidrazil, DPPH•) analizi sonucunda, bitkinin çiçek-yaprak kısmının asetik asit-su ekstraksiyonunda, en yüksek radikal süpürme değeri olarak 10.697±0.155 mM Troloks eşdeğeri (TE)/g Kuru Madde (KM) bulunmuş ve ekstrakt konsantrasyonu arttıkça DPPH inhibisyonunun arttığı gözlemlenmiştir. Disk difüzyon metoduyla yapılan antimikrobiyel aktivite sonuçlarına göre, tüm ekstraktlar kıyaslandığında, bitkinin çiçek-yaprak kısmının etil alkol ile elde edilmiş ektraktı ile en yüksek antimikrobiyel etkinin 25.33±0.57 mm zon çapı ile Proteus vulgaris’a karşı olduğu tespit edilmiştir.

Anahtar Kelimeler

Antimikrobiyel aktivite, Biyoaktivite, Lythrum salicaria L., Toplam fenolik madde

Determination of total phenolic compound, antioxidant and antimicrobial activities of purple loosestrife (Lythrum salicaria l.) plant

Öz

Lythrum salicaria L. known as “Kırmızı (Tıbbi) hevhulma” in Turkish is used for its several beneficial health effects against diarrhea, chronic intestinal fever, gingival bleeding, hemorrhoids and eczema. In this study, dried herbal parts of Lythrum salicaria L. (Lythraceae) were sequentially extracted with different solvents such as ethyl alcohol and acetic acid-water, respectively. Total amount of phenolic substances of all extracts were determined and antioxidant and antimicrobial activities of all the extracts were investigated using in vitro methods. Consequently, because of Folin-Ciocalteu method applied to determine the total amount of phenolic substances, the highest total amount of phenolic compounds was determined in the acetic acid-water extract of the parts of flower-leaf of plant and it was found to be 5.916± 0.335 mg Gallic Asid Eqivalent (GAE)/g. As a result of free radical scavenging activity (1,1-diphenyl-2-picrilhydrazyl, DPPH•) analysis, 10.697 ± 0.155 mM Trolox equivalent (TE) /g Dry Matter (DM) was found in the acetic acid-water extract of the flower-leaf part of the plant and it was observed that DPPH inhibition increased as the extract concentration increased. According to the results of antimicrobial activity performed by the disk diffusion method, it was determined that the highest antimicrobial effect against the Proteus vulgaris with the ethanol extract of the flower-leaf part of the stem of the plant and the zone diameter was found as 25.33 ± 0.57 mm.

Anahtar Kelimeler

Antimicrobial activity, Bioactivity, Lythrum salicaria L., Total phenolic compounds

Kaynakça

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