Doksorubisin ile ilişkili kardiyotoksisitede miR-130a-3p’nin potansiyel hedef genlerinin araştırılması ve tanımlanması için biyoinformatik bir yaklaşım

Yıl 2021, Cilt: 11 Sayı: 3, 964 - 974, 15.07.2021

Hamid Ceylan

https://doi.org/10.17714/gumusfenbil.842966

Cited By: 1

Öz

Doksorubisin (DOX), en etkili kemoterapi ilaçlarından biri olarak kabul edilmekte ve birçok kanser türünü tedavi etmek için kullanılmaktadır. Öte yandan, bu ilacın etkinliği, kardiyotoksisite gibi olumsuz yan etkileri nedeniyle sınırlıdır. Bununla birlikte, DOX kaynaklı kardiyotoksisitenin kesin mekanizması tam olarak anlaşılmamıştır. Bu çalışmada, İlaca bağlı kardiyotoksisitenin moleküler mekanizmasını daha iyi anlamak için kalbe özgü bir miRNA'yı ve kalpteki hedef genlerini belirlemeye odaklanmıştır. Herkese açık veriler, Gene Expression Omnibus (GEO) veri tabanından indirildi ve farklı şekilde ifade edilen genler (DEG'ler), çevrimiçi biyoinformatik aracı iPathwayGuide kullanılarak belirlendi. Sekiz farklı tahmin aracı kullanılarak miR-130a-3p'nin hedef genleri belirlendi. miR-130a-3p hedefleriyle kesişen DEG'ler için gen ontolojisi (GO) ve Kyoto Encyclopedia of Genes and Genomes (KEGG) yolu zenginleştirme analizi yapıldı. Kalp yetmezliği (HF) olan ve HF olmayan gruplar arasında, aynı zamanda miR-130a-3p'nin de hedefi olan toplam 29 DEG tespit edildi. Kesişen genler arasında yer alan SLC8A1'in bozulmuş miyokardiyal fonksiyon ve kardiyotoksisitede şekillenen çok önemli bir gen olabileceğini bulundu. Çalışmanın bulguları, DOX kaynaklı kardiyotoksisitenin teşhisi, tedavisi ve / veya azaltılması için potansiyel hedeflere yeni bilgiler sağlamaktadır.

Anahtar Kelimeler

Biyoinformatik analiz, Kardiyotoksisite, Farklı şekilde ifade edilen genler, Doksorubisin, mikroRNA

Destekleyen Kurum

Yok

Proje Numarası

Yok

Teşekkür

Yok

A bioinformatics approach for exploring and identification of potential target genes of miR-130a-3p in doxorubicin-associated cardiotoxicity

Öz

Doxorubicin (DOX) is considered one of the most effective chemotherapy drug and is used to treat many types of cancer. On the other hand, the effectiveness of this drug is restricted due to its adverse effects such as cardiotoxicity. However, the exact mechanism of DOX-induced cardiotoxicity has not been fully understood. To better understand the molecular mechanism of DOX-induced cardiotoxicity, this study focused on identifying a heart-specific miRNA and its target genes in the heart. Publicly available data was downloaded from the Gene Expression Omnibus (GEO) database, and differentially expressed genes (DEGs) were extracted by using the online bioinformatics tool iPathwayGuide. Using eight different prediction tools, target genes of miR-130a-3p were identified. Gene ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway enrichment analyses were performed for DEGs intersected with miR-130a-3p targets. Among the HF and non-HF groups, a total of 29 DEGs targeted by miR-130a-3p were identified. We found that SLC8A1, which is among the intersecting genes, might be a crucial gene that is shaped in impaired myocardial function and cardiotoxicity. In conclusion, the findings of the study provided new insights into the potential targets for DOX-induced cardiotoxicity diagnosis, treatment, and/or attenuation.

Anahtar Kelimeler

Bioinformatics analysis, Cardiotoxicity, Differentially expressed genes, Doxorubicin, microRNA

Proje Numarası

Yok

Kaynakça

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