Carbonic anhydrase inhibition and antioxidant activity of the axially naphthoxazin group substituted silicon phthalocyanines

Yıl 2022, Cilt: 12 Sayı: 1, 227 - 234, 15.01.2022

Ayça Aktaş Karaçelik , Volkan Çakır , Hüseyin Baş Zekeriya Bıyıklıoğlu

https://doi.org/10.17714/gumusfenbil.1001784

Öz

Silicon phthalocyanines are an interesting subclass of phthalocyanines. They are abundant and have extremely low toxicity levels. The low solubility of silicon phthalocyanine is the major obstacle to its use in many different applications. Therefore, in a previous study, two axially substituted silicon phthalocyanines were synthesized to increase their solubility. In this study, these axially substituted silicon phthalocyanines were evaluated for carbonic anhydrase inhibition and antioxidant activities. The carbonic anhydrase (CA) inhibition potential of silicon phthalocyanines was evaluated by esterase activity. The antioxidant activity was tested by 2,2-diphenyl-1-picrylhydrazyl (DPPH) radical scavenging and ferric ion (III) reducing/antioxidant power (FRAP) assays. The silicon phthalocyanines had significant CA inhibitory activity [50% inhibitory values (IC50): 495 ± 12.74 nM and 857 ± 13.03 nM for H4-Si and H3-Si, respectively]. According to the antioxidant studies, 50% scavenging concentration (SC50) values of DPPH• assay were 2.29 ± 0.06 µg/mL and 1.39 ± 0.43 µg/mL for H3-Si and H4-Si and Trolox Equivalent Antioxidant Capacity (TEAC) values of FRAP test were 259.33 ± 48.27 µM and 342.00 ± 44.40 for H3-Si µM and H4-Si, respectively. Consequently, silicon phthalocyanine compounds are considered to have great potential for their use in various fields such as food and medicine.

Anahtar Kelimeler

Antioxidant, Carbonic anhydrase, Esterase activity, Inhibitor, Silicon phthalocyanines

Eksenel olarak naftoksazin grubu sübstitüe edilmiş silisyum ftalosiyaninlerin karbonik anhidraz inhibisyonu ve antioksidan aktivitesi

Öz

Silisyum ftalosiyaninler, ftalosiyaninlerin ilgi duyulan bir alt sınıfıdır. Bol miktarda bulunurlar ve son derece düşük toksisite seviyelerine sahiptirler. Silisyum ftalosiyanininin düşük çözünürlüğü, birçok farklı uygulamada kullanımının önündeki en büyük engeldir. Bu yüzden, çözünürlüklerini artırmak için eksenel olarak sübstitüe edilmiş iki silisyum ftalosiyanin daha önce yapılan bir çalışmada sentezlendi. Bu çalışmada, eksenel olarak sübstitüe edilmiş bu silisyum ftalosiyaninler, karbonik anhidraz inhibisyonu ve antioksidan aktiviteleri açısından değerlendirildi. Silisyum ftalosiyaninlerin karbonik anhidraz (CA) inhibisyon potansiyeli, esteraz aktivitesi ile değerlendirildi. Antioksidan aktivite, 2,2-difenil-1-pikrilhidrazil (DPPH•) radikal temizleme ve demir iyon (III) indirgeme / antioksidan güç (FRAP) metotları ile test edildi. Silisyum ftalosiyaninler, önemli CA inhibitör aktivitesine [%50 inhibitör değerleri (IC50): H4-Si ve H3-Si için sırasıyla 495 ± 12.74 nM ve 857 ± 13.03 nM] sahipti. Antioksidan çalışmalara göre, DPPH• testinin %50 temizleme konsantrasyon (SC50) değerleri sırasıyla H3-Si için 2.29 ± 0.06 µg/mL, H4-Si için 1.39 ± 0.43 µg/mL ve FRAP testinin Trolox Eşdeğer Antioksidan Kapasitesi (TEAC) değerleri H3-Si için 259.33±48.27 µM, H4-Si için 342.00 ± 44.40 µM olarak bulundu. Sonuç olarak, silisyum ftalosiyanin bileşiklerinin, gıda ve tıp gibi çeşitli alanlarda kullanımları için büyük potansiyele sahip olduğu düşünülmektedir.

Anahtar Kelimeler

Antioksidan, Karbonik anhidraz, Esteraz aktivitesi, İnhibitör, Silisyum ftalosiyaninler

Kaynakça

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