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Karnabahar ve brokoli fidelerine yapılan melatonin uygulamalarının tuz stresi üzerine etkisi

Yıl 2022, , 181 - 192, 23.06.2022
https://doi.org/10.29050/harranziraat.1065707

Öz

Bu çalışma serin iklim sebze yetiştiriciliğinde önemli bir yere sahip olan karnabahar ve brokoliye dışarıdan yapılan melatonin (MEL) uygulamalarının tuz stresi üzerine olası etkilerini belirleyebilmek amacıyla gerçekleştirilmiştir. Bu sebeple 4-6 gerçek yapraklı karnabahar ve brokoli fidelerine farklı dozlarda MEL (0, 5 ve 10 μM MEL) içeren sulu çözelti topraktan uygulanmıştır. Tesadüf parselleri deneme desenine göre 3 tekerrürlü olarak kurulan denemede stres etkilerini belirleyebilmek amacıyla fidelerde fiziksel (fide ağırlıkları, boyu, çapı ve yaprak alanı) ve biyokimyasal (elektriki iletkenlik, klorofil ve karotenoid içeriği, prolin, melondialdehit ve toplam fenolik madde içeriği) analizler gerçekleştirilmiştir. Analizler sonucunda stres altındaki fidelere dışarıdan yapılan MEL uygulamalarının stresin olumsuz etkilerini azaltarak boy, çap, ağırlık, klorofil, karetenoid, antosiyanin ve fenolik madde içeriğinde iyileşmeler sağladığı gözlemlenmiştir. Sonuç olarak dışarıdan yapılan MEL uygulamaları tuz stresinin olumsuz etkilerini azaltmada etkili olmuş, bununla birlikte karnabaharda 5μM MEL uygulaması, brokoli fidelerinde ise 5μM-10 μM MEL uygulamaları uygun dozlar olarak belirlenmiştir.

Destekleyen Kurum

Tübitak

Proje Numarası

2209-A Üniversite Öğrencileri Araştırma Projeleri

Teşekkür

Bu çalışma Tübitak 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında desteklenmiştir. Desteklerinden dolayı teşekkür ederiz.

Kaynakça

  • Ahmad, S., Cui, W., Kamran, M., Ahmad, I., Meng, X., Wu, X., Su, W., Javed, T., El-Serehy A. H., Jia, Z., & Han, Q. (2021). Exogenous application of melatonin induces tolerance to salt stress by improving the photosynthetic efficiency and antioxidant defense system of maize seedling. Journal of Plant Growth Regulation, 40(3), 1270-1283. https://doi.org/10.1007/s00344-020-10187-0
  • Altaf, M. A., Shahid, R., Ren, M. X., Naz, S., Altaf, M. M., Qadir, A., Anwar, M., Shakoor, A. & Hayat, F. (2020). Exogenous melatonin enhances salt stress tolerance in tomato seedlings. Biologia Plantarum, 64, 604-615.
  • Arnao, M.B., Hernandez-Ruiz, J. (2014). Melatonin: plant growth regulator and/or biostimulator during stress? Trends in Plant Science 19 (12): 789-797. doi: https://doi.org/10.1016/j.tplants.2014.07.006.
  • Arnao, M.B., Hernandez-Ruiz, J., 2015a. Functions of melatonin in plants: a review. Journal of Pineal Research, 59: 133–150.
  • Arnao, M.B., Hernandez-Ruiz, J. 2015b. Phytomelatonin: searching for plants with high levels for use as natural nutraceutical. Studies in Natural Products Chemistry, 46: 523-549.
  • Arnao, M. B., Hernández-Ruiz, J. 2020. “Is phytomelatonin a new plant hormone?”, Agronomy, 101, 95. https://doi.org/10.3390/agronomy10010095
  • Ashraf, M. F. M. R., & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59(2), 206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006
  • Balkaya, A (2011). Lahanagil Yetiştiriciliği. Bahçe Tarımı 2. Anadolu Üniversitesi Açıköğretim Fakültesi Yayın No: 1355, 148-150.
  • Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39(1), 205-207. https://doi.org/10.1007/BF000 18060
  • Boursiac, Y., Chen, S., Luu, D. T., Sorieul, M., van den Dries, N., & Maurel, C. (2005). Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant physiology, 139(2), 790-805. https://doi.org/10.1104/pp.105.065029
  • Campos, C. N., Ávila, R. G., de Souza, K. R. D., Azevedo, L. M., & Alves, J. D. (2019). Melatonin reduces oxidative stress and promotes drought tolerance in young Coffea arabica L. plants. Agricultural Water Management, 211, 37-47.
  • Çulha, Ş., & Çakırlar, H. (2011). Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 11(2), 11-34. Retrieved from https://dergipark.org.tr/en/pub/akufemubid/issue/1591/19755
  • Eryılmaz, F. (2006). The relationships between salt stress and anthocyanin content in higher plants. Biotechnology & Biotechnological Equipment, 20(1), 47-52.
  • Günay, A. (1984). Sebzecilik: Özel Sebze Yetistiriciligi. Çag Matbaası, Ankara, 312.
  • Güneş, A., Inal, A., Bagci, E. G., & Pilbeam, D. J. (2007). Silicon-mediated changes of some physiological and enzymatic parameters symptomatic for oxidative stress in spinach and tomato grown in sodic-B toxic soil. Plant and Soil, 290(1), 103-114. https://doi.org/10.1007/s11104-006-9137-9
  • Hernandez, I. G., Gomez, F. J. V., Cerutti, S., Arana, M. V., & Silva, M. F. (2015). Melatonin in Arabidopsis thaliana acts as plant growth regulator at low concentrations and preserves seed viability at high concentrations. Plant Physiology and Biochemistry, 94, 191-196. https://doi.org/10.1016/j.plaphy.2015.06.011
  • Horie, T., Kaneko, T., Sugimoto, G., Sasano, S., Panda, S. K., Shibasaka, M., & Katsuhara, M. (2011). Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots. Plant and Cell Physiology, 52(4), 663-675. https://doi.org/10.1093/pcp/pcr027
  • Jiang, C., Cui, Q., Feng, K., Xu, D., Li, C., & Zheng, Q. (2016). Melatonin improves antioxidant capacity and ion homeostasis and enhances salt tolerance in maize seedlings. Acta physiologiae plantarum, 38(4), 82. https://doi.org/10.1007/s11738-016-2101-2
  • Karaca, A. 2013. “Dışarıdan yapılan melatonin uygulamaları ile biberde çimlenme sırasında üşüme stresine karşı toleransın arttırılması”, Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilimdalı Yüksek Lisans Tezi, Kahramanmaraş.
  • Ke, Q., Ye, J., Wang, B., Ren, J., Yin, L., Deng, X., & Wang, S. (2018). Melatonin mitigates salt stress in wheat seedlings by modulating polyamine metabolism. Frontiers in Plant Science, 9, 914.
  • Kirk, J. T. O., & Allen, R. L. (1965). Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochemical and Biophysical Research Communications, 21(6), 523-530. https://doi.org/10.1016/0006-291X(65)90516-4
  • Korkmaz, A., Değer, Ö., Szafrańska, K., Köklü, Ş., Karaca, A., Yakupoğlu, G., & Kocacinar, F. (2021). Melatonin effects in enhancing chilling stress tolerance of pepper. Scientia Horticulturae, 289, 110434.
  • Korkmaz, A., Uzunlu, M., & Demirkiran, A. R. (2007). Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress. Acta Physiologiae Plantarum, 29(6), 503-508. https://doi.org/10.1007/s11738-007-0060-3
  • Korkmaz, A., Yakupoğlu, G., Köklü, Ş., Cuci, Y., & Kocacinar, F. (2017). Determining diurnal and seasonal changes in melatonin andtryptophan contents of eggplant (Solanum melongena L.). Turkish Journal of Botany, 41(4), 356-366. https://doi.org/10.3906/bot-1611-48
  • Köklü, Ş. (2016). Melatoninin biber tohumlarının yaşlanması üzerine etkilerinin incelenmesi. KSÜ. Fen Bil. Ens., Bahçe Bitkileri Bölümü, Yüksek Lisans Tezi, 98s.
  • Lazár, D., Murch, S. J., Beilby, M. J., & Al Khazaaly, S. (2013). Exogenous melatonin affects photosynthesis in characeae Chara australis. Plant Signaling & Behavior, 8(3), 23279.
  • Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., ... & Ma, F. (2012). The mitigation effects of exogenous melatonin on salinity‐induced stress in Malus hupehensis. Journal of Pineal Research, 53(3), 298-306. https://doi.org/10.1111/j.1600-079X.2012.00999.x
  • Li, C., Tan, D. X., Liang, D., Chang, C., Jia, D., & Ma, F. (2015). Melatonin mediates the regulation of ABA metabolism, free-radical scavenging, and stomatal behaviour in two Malus species under drought stress. Journal of Experimental Botany, 66(3), 669-680.
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The effect of melatonin treatments on cauliflower and broccoli seedlings on salt stress

Yıl 2022, , 181 - 192, 23.06.2022
https://doi.org/10.29050/harranziraat.1065707

Öz

This study was carried out to determine the possible effects of exogen melatonin (MEL) treatments on salt stress in cauliflower and broccoli, which have a significant position among cool region vegetables. For that reason, an aqueous solution containing at different concentrations MEL (0, 5, and 10 μM MEL) were applied to the cauliflower and broccoli seedlings with 4-6 true leaves from the soil. In this experiment, which was established with 3 replications according to the randomized plots trial design, physical (seedling weight, height, diameter, and leaf area) and biochemical (electrical conductivity, chlorophyll and carotenoid content, proline, malondialdehyde, and total phenolic substance content) analyzes were carried out to determine the stress effects on the seedlings. As a result of the analyzes, it was observed that exogen MEL treatments to the seedlings under stress reduced the unfavorable effects of stress and provided improvements in height, diameter, weight, chlorophyll, carotenoid, anthocyanin, and phenolic substance content. As a result, exogen MEL treatments were very efficient in reducing the unfavorable effects of salt stress. However, 5μM MEL treatment in cauliflower and 5 and 10 μM MEL tratments in broccoli seedlings were determined as appropriate concentrations.

Proje Numarası

2209-A Üniversite Öğrencileri Araştırma Projeleri

Kaynakça

  • Ahmad, S., Cui, W., Kamran, M., Ahmad, I., Meng, X., Wu, X., Su, W., Javed, T., El-Serehy A. H., Jia, Z., & Han, Q. (2021). Exogenous application of melatonin induces tolerance to salt stress by improving the photosynthetic efficiency and antioxidant defense system of maize seedling. Journal of Plant Growth Regulation, 40(3), 1270-1283. https://doi.org/10.1007/s00344-020-10187-0
  • Altaf, M. A., Shahid, R., Ren, M. X., Naz, S., Altaf, M. M., Qadir, A., Anwar, M., Shakoor, A. & Hayat, F. (2020). Exogenous melatonin enhances salt stress tolerance in tomato seedlings. Biologia Plantarum, 64, 604-615.
  • Arnao, M.B., Hernandez-Ruiz, J. (2014). Melatonin: plant growth regulator and/or biostimulator during stress? Trends in Plant Science 19 (12): 789-797. doi: https://doi.org/10.1016/j.tplants.2014.07.006.
  • Arnao, M.B., Hernandez-Ruiz, J., 2015a. Functions of melatonin in plants: a review. Journal of Pineal Research, 59: 133–150.
  • Arnao, M.B., Hernandez-Ruiz, J. 2015b. Phytomelatonin: searching for plants with high levels for use as natural nutraceutical. Studies in Natural Products Chemistry, 46: 523-549.
  • Arnao, M. B., Hernández-Ruiz, J. 2020. “Is phytomelatonin a new plant hormone?”, Agronomy, 101, 95. https://doi.org/10.3390/agronomy10010095
  • Ashraf, M. F. M. R., & Foolad, M. R. (2007). Roles of glycine betaine and proline in improving plant abiotic stress resistance. Environmental and Experimental Botany, 59(2), 206-216. https://doi.org/10.1016/j.envexpbot.2005.12.006
  • Balkaya, A (2011). Lahanagil Yetiştiriciliği. Bahçe Tarımı 2. Anadolu Üniversitesi Açıköğretim Fakültesi Yayın No: 1355, 148-150.
  • Bates, L. S., Waldren, R. P., & Teare, I. D. (1973). Rapid determination of free proline for water-stress studies. Plant and soil, 39(1), 205-207. https://doi.org/10.1007/BF000 18060
  • Boursiac, Y., Chen, S., Luu, D. T., Sorieul, M., van den Dries, N., & Maurel, C. (2005). Early effects of salinity on water transport in Arabidopsis roots. Molecular and cellular features of aquaporin expression. Plant physiology, 139(2), 790-805. https://doi.org/10.1104/pp.105.065029
  • Campos, C. N., Ávila, R. G., de Souza, K. R. D., Azevedo, L. M., & Alves, J. D. (2019). Melatonin reduces oxidative stress and promotes drought tolerance in young Coffea arabica L. plants. Agricultural Water Management, 211, 37-47.
  • Çulha, Ş., & Çakırlar, H. (2011). Tuzluluğun bitkiler üzerine etkileri ve tuz tolerans mekanizmaları. Afyon Kocatepe Üniversitesi Fen ve Mühendislik Bilimleri Dergisi, 11(2), 11-34. Retrieved from https://dergipark.org.tr/en/pub/akufemubid/issue/1591/19755
  • Eryılmaz, F. (2006). The relationships between salt stress and anthocyanin content in higher plants. Biotechnology & Biotechnological Equipment, 20(1), 47-52.
  • Günay, A. (1984). Sebzecilik: Özel Sebze Yetistiriciligi. Çag Matbaası, Ankara, 312.
  • Güneş, A., Inal, A., Bagci, E. G., & Pilbeam, D. J. (2007). Silicon-mediated changes of some physiological and enzymatic parameters symptomatic for oxidative stress in spinach and tomato grown in sodic-B toxic soil. Plant and Soil, 290(1), 103-114. https://doi.org/10.1007/s11104-006-9137-9
  • Hernandez, I. G., Gomez, F. J. V., Cerutti, S., Arana, M. V., & Silva, M. F. (2015). Melatonin in Arabidopsis thaliana acts as plant growth regulator at low concentrations and preserves seed viability at high concentrations. Plant Physiology and Biochemistry, 94, 191-196. https://doi.org/10.1016/j.plaphy.2015.06.011
  • Horie, T., Kaneko, T., Sugimoto, G., Sasano, S., Panda, S. K., Shibasaka, M., & Katsuhara, M. (2011). Mechanisms of water transport mediated by PIP aquaporins and their regulation via phosphorylation events under salinity stress in barley roots. Plant and Cell Physiology, 52(4), 663-675. https://doi.org/10.1093/pcp/pcr027
  • Jiang, C., Cui, Q., Feng, K., Xu, D., Li, C., & Zheng, Q. (2016). Melatonin improves antioxidant capacity and ion homeostasis and enhances salt tolerance in maize seedlings. Acta physiologiae plantarum, 38(4), 82. https://doi.org/10.1007/s11738-016-2101-2
  • Karaca, A. 2013. “Dışarıdan yapılan melatonin uygulamaları ile biberde çimlenme sırasında üşüme stresine karşı toleransın arttırılması”, Kahramanmaraş Sütçü İmam Üniversitesi Fen Bilimleri Enstitüsü, Bahçe Bitkileri Anabilimdalı Yüksek Lisans Tezi, Kahramanmaraş.
  • Ke, Q., Ye, J., Wang, B., Ren, J., Yin, L., Deng, X., & Wang, S. (2018). Melatonin mitigates salt stress in wheat seedlings by modulating polyamine metabolism. Frontiers in Plant Science, 9, 914.
  • Kirk, J. T. O., & Allen, R. L. (1965). Dependence of chloroplast pigment synthesis on protein synthesis: effect of actidione. Biochemical and Biophysical Research Communications, 21(6), 523-530. https://doi.org/10.1016/0006-291X(65)90516-4
  • Korkmaz, A., Değer, Ö., Szafrańska, K., Köklü, Ş., Karaca, A., Yakupoğlu, G., & Kocacinar, F. (2021). Melatonin effects in enhancing chilling stress tolerance of pepper. Scientia Horticulturae, 289, 110434.
  • Korkmaz, A., Uzunlu, M., & Demirkiran, A. R. (2007). Treatment with acetyl salicylic acid protects muskmelon seedlings against drought stress. Acta Physiologiae Plantarum, 29(6), 503-508. https://doi.org/10.1007/s11738-007-0060-3
  • Korkmaz, A., Yakupoğlu, G., Köklü, Ş., Cuci, Y., & Kocacinar, F. (2017). Determining diurnal and seasonal changes in melatonin andtryptophan contents of eggplant (Solanum melongena L.). Turkish Journal of Botany, 41(4), 356-366. https://doi.org/10.3906/bot-1611-48
  • Köklü, Ş. (2016). Melatoninin biber tohumlarının yaşlanması üzerine etkilerinin incelenmesi. KSÜ. Fen Bil. Ens., Bahçe Bitkileri Bölümü, Yüksek Lisans Tezi, 98s.
  • Lazár, D., Murch, S. J., Beilby, M. J., & Al Khazaaly, S. (2013). Exogenous melatonin affects photosynthesis in characeae Chara australis. Plant Signaling & Behavior, 8(3), 23279.
  • Li, C., Wang, P., Wei, Z., Liang, D., Liu, C., Yin, L., ... & Ma, F. (2012). The mitigation effects of exogenous melatonin on salinity‐induced stress in Malus hupehensis. Journal of Pineal Research, 53(3), 298-306. https://doi.org/10.1111/j.1600-079X.2012.00999.x
  • Li, C., Tan, D. X., Liang, D., Chang, C., Jia, D., & Ma, F. (2015). Melatonin mediates the regulation of ABA metabolism, free-radical scavenging, and stomatal behaviour in two Malus species under drought stress. Journal of Experimental Botany, 66(3), 669-680.
  • Li, H., Chang, J., Chen, H., Wang, Z., Gu, X., Wei, C., Zhang, Y., Ma, J., Yang, J. & Zhang, X. (2017). Exogenous melatonin confers salt stress tolerance to watermelon by improving photosynthesis and redox homeostasis. Frontiers in plant science, 8, 295.
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  • Liang, W., Ma, X., Wan, P., & Liu, L. (2018). Plant salt-tolerance mechanism: A review. Biochemical and biophysical research communications, 495(1), 286-291. https://doi.org/10.1016/j.bbrc.2017.11.043
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  • Martínez-Ballesta, M., Martínez, V., & Carvajal, M. (2000). Regulation of water channel activity in whole roots and in protoplasts from roots of melon plants grown under saline conditions. Functional Plant Biology, 27(7), 685-691. https://doi.org/10.1071/PP99203
  • Martinez, V., Nieves-Cordones, M., Lopez-Delacalle, M., Rodenas, R., Mestre, T. C., Garcia-Sanchez, F., Rubio, F., Nortes, P.A., Mittler, R., & Rivero, R. M. (2018). Tolerance to stress combination in tomato plants: new insights in the protective role of melatonin. Molecules, 23(3), 535.
  • Munns, R., James, R. A., & Läuchli, A. (2006). Approaches to increasing the salt tolerance of wheat and other cereals. Journal of experimental botany, 57(5), 1025-1043. https://doi.org/10.1093/jxb/erj100
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  • Nguyen, H. C., Lin, K. H., Ho, S. L., Chiang, C. M., & Yang, C. M. (2018). Enhancing the abiotic stress tolerance of plants: from chemical treatment to biotechnological approaches. Physiologia plantarum, 164(4), 452-466. https://doi.org/10.1111/ppl.128 12.
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  • Park, H. S., Kazerooni, E. A., Kang, S. M., Al-Sadi, A. M., & Lee, I. J. (2021). Melatonin enhances the tolerance and recovery mechanisms in Brassica juncea (L.) Czern. under saline conditions. Frontiers in Plant Science, 12. https://doi.org/10.3389/fpls.2021.593 717
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  • Zhang, T., Shi, Z., Zhang, X., Zheng, S., Wang, J., & Mo, J. (2020). Alleviating effects of exogenous melatonin on salt stress in cucumber. Scientia Horticulturae, 262, 109070.
  • Zhao, L., An, R., Yang, Y., Yang, X., Liu, H., Yue, L., Li, X., Lin, Y., Reiter, R. J. & Qu, Y. (2015). Melatonin alleviates brain injury in mice subjected to cecal ligation and puncture via attenuating inflammation, apoptosis, and oxidative stress: the role of SIRT 1 signaling. Journal of Pineal Research, 59(2), 23
Toplam 59 adet kaynakça vardır.

Ayrıntılar

Birincil Dil Türkçe
Konular Bahçe Bitkileri Yetiştirme ve Islahı
Bölüm Araştırma Makaleleri
Yazarlar

Yasin Arslan 0000-0002-6084-5238

Şebnem Köklü 0000-0002-5769-2963

Gökçen Yakupoğlu 0000-0003-4921-0925

Proje Numarası 2209-A Üniversite Öğrencileri Araştırma Projeleri
Yayımlanma Tarihi 23 Haziran 2022
Gönderilme Tarihi 31 Ocak 2022
Yayımlandığı Sayı Yıl 2022

Kaynak Göster

APA Arslan, Y., Köklü, Ş., & Yakupoğlu, G. (2022). Karnabahar ve brokoli fidelerine yapılan melatonin uygulamalarının tuz stresi üzerine etkisi. Harran Tarım Ve Gıda Bilimleri Dergisi, 26(2), 181-192. https://doi.org/10.29050/harranziraat.1065707

Derginin Tarandığı İndeksler

13435  19617   22065  13436  134401344513449 13439 13464  22066   22069  13466 

10749 Harran Tarım ve Gıda Bilimi Dergisi, Creative Commons Atıf –Gayrı Ticari 4.0 Uluslararası (CC BY-NC 4.0) Lisansı ile lisanslanmıştır.