Cydalima perspectalis (Walker) (Lepidoptera: Crambidae) üzerinde potansiyel biyolojik mücadele etmeni Bracon hebetor (Say) (Hymenoptera: Braconidae)’un etkinliği

Yıl 2023, , 362 - 371, 27.09.2023

Hilal Tunca , Cansu Kandil , Damla Çayci , Benjamin Cosic , Özgür Toprak

https://doi.org/10.29050/harranziraat.1261385

Cited By: 1

Öz

Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) birçok ülkede şimşirlerin ana zararlısı konumundadır. Türkiye’de son 6-7 yıldır önemli zarara neden olmaktadır. Bu zararlıya karşı şimşirlerin özellikle ormanlarda, park ve bahçelerde bulunması nedeniyle kimyasal mücadele önerilmemektedir. Bu çalışmada, biyolojik mücadele kapsamında idiobiont larva parazitoiti Bracon hebetor (Say, 1836) (Hymenoptera: Braconidae)’un şimşir güvesine karşı potansiyel kullanımı değerlendirilmiştir. Laboratuvar çalışmaları genç ve olgun dönem C. perspectalis larvaları ile 25±1°C, % 60-70R.H. ve 16:8 (aydınlık:karanlık) koşullarda gerçekleştirilmiştir. Paralize olan ve parazitlenen larva sayıları, parazitleme oranı, dişi başına bırakılan yumurta sayısı ile larva ve pupa oranları belirlenmiştir. B. hebetor paraliz etme yeteneğine sahip olup bu sayede zararlı C. perspectalis’e karşı insektisit etkisi göstermektedir. Üçüncü dönemde paralize olan larva sayısı (10.33±1.15) beşinci döneme (4.67±0.57) göre daha yüksek bulunmuştur. Parazitlenen larva (14.68±1.50) ve bırakılan yumurta sayısı (7.50±0.32) beşinci dönem larvalarda daha yüksektir. Parazitleme oranı erken ve geç dönem larvalarda sırasıyla %33 ve %73 olarak bulunmuştur. Sözkonusu biyolojik veriler ilk defa elde edilmiştir. Bracon hebetor’un venomu Cydalima larvaları üzerine yüksek derecede toksik etki yapmıştır. Çalışma sonuçları, şimşir güvesine karşı kullanılacak kimyasal mücadeleye alternatif olarak bu parazitoitin kullanılabileceğini göstermektedir.

Anahtar Kelimeler

biyolojik mücadele, Bracon hebetor, Cydalima perspectalis

Effectiveness of potential biological control agent Bracon hebetor (Say) (Hymenoptera: Braconidae) on Cydalima perspectalis (Walker) (Lepidoptera: Crambidae)

Öz

Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) is the main pest of boxwood fields in many countries. It has caused significant damage especially since 6-7 years in Türkiye. Chemical control is definitely not recommended against this pest because boxwoods are found in forest areas, parks and gardens. In this study, the potential use of the idibiont larval parasitoid Bracon hebetor (Say, 1836) (Hymenoptera: Braconidae) against the box tree moth was evaluated within the scope of biological control. Laboratory experiments were carried out with early and late instar larvae of C. perspectalis at 25±1°C, 60-70% R.H. and 16:8 (light: dark) conditions. The number of paralyzed and parasitized larvae, parasitism rate, number of eggs laid per female, rate of larvae and pupae were determined. Bracon hebetor, which has the ability to paralyze the host larvae, showed an insecticidal effect against the pest C. perspectalis. It was found that the number of paralyzed larvae (10.33±1.15) in early stage (3rd instar) larvae was higher than in late stage (5th instar) larvae (4.67±0.57). On the other hand, it was determined that the number of parasitized larvae (14.68±1.50) and eggs laid (7.50±0.32) was higher in late stage (5th instar) larvae. Parasitism rates were found to be 33% and 73% in early and late instar larvae, respectively. This detailed biological data has been obtained to for the first time. Bracon hebetor venom showed a high toxic effect on Cydalima larvae. The results of the study showed that the parasitoid may be recommended as an alternative to chemical control against the box tree moth.

Anahtar Kelimeler

bological control, Bracon hebetor, Cydalima perspectalis

Kaynakça

• Antolin, M. F., Ode, P. J., Strand, M. R. (1995). Variable sex ratio and ovicide in an out breeding parasitic wasp. Animal Behaviour, 49 (3): 589-600. https://doi.org/10.1016/0003-3472(95)80192-8
• Barbosa, P. (1988). Natural enemies and herbivore-plant interactions: influence of plant allelochemicals and host specificity. In: P. Barbosa, & D. K. Letourneau (Eds), Novel aspects of insect-plant interactions (pp 201–229) Wiley: New York.
• Billen, W. (2007). Diaphania perspectalis (Lepidoptera: Pyrallidae)-a new moth in Europe. Mitteilungen der Entomologischen Gesellschaft Basel, 57 (2/4): 135-137.
• Bird, S., Raper, C., Dale-Skey, N., Salisbury, A. (2020). First records of two natural enemies of box tree moth, Cydalima perspectalis (Lepidoptera: Crambidae), in Britain. British Journal of Entomology & Natural History 33 (1): 67-70.
• Blaschek, W., Ebel, S. (2007). Hagers Enzyklopädie der Arzneistoffe und Drogen. Mit 600 Tabellen. Wiss. Verl.-Ges; Stuttgart, Germany: neu bearb. und erg. Aufl.
• Brua, C. (2013). La pyrale du buis, Cydalima perspectalis (Walker, 1859), espece exotique envahissante, caracteristiques de sa dynamique d’expansion en France et en Europe, des degats occasionnes sur les buis (Buxus spp) et des strategies de lutte.– 3e Conférence sur l’Entretien des Espaces Verts, Hardins, Gazons, Forêts, Zones Aquatiques et Autres Zones Non Agricoles. Association Française de Protection des Plantes (AFPP). (pp 365-377).
• Bukovinsky, T., Poelman, E. H., Gols, R., Prekatsakis, G., Vet, L. E. M., Harvey, J. A., Dicke, M. (2009). Consequences of constitutive and induced variation in plant nutritional quality for immune defence of a herbivore against parasitism. Oecologia, 160: 299-308. https://doi.org/10.1007/s00442-009-1308-y
• Charnov, E. L. (1982). The theory of sex allocation. (MPB-18), Volume 18 .Princeton University Press, Princeton, NJ, 355 pp.
• Demiray, E. (2021). Farklı Besinlerde Yetiştirilen Ephestia kuehniella (Lepidoptera: Pyralidae) Üzerinde Parazitoit Bracon hebetor (Hymenoptera: Braconidae)’ un Bazı Biyolojik Özellikleri Üzerine Araştırmalar. Ankara Üniversitesi Fen Bilimleri Enstitüsü, Yüksek Lisans Tezi, s 29.
• Di Vitantonio, C. (2016). Natural and anthropogenic factors affecting the life cycle of exotic and native insect species. PhD Thesis, Universita di Bologna Bologna.
• Farahani, S., Salehi, M., Farashiani, M. E., Gilasian, E., Terujeni, S. N. K., Ahangaran, Y. (2018). Compsilura concinnata (Meigen), parasitoid of box tree moth, Cydalima perspectalis (Walker) from Iran. Iranian Journal of Forest Range Protection Research, 16 (1): 102-105. 1336.122038.2018.ijfrpr/22092.1
• Feás Sánchez, X., Charles, R. J. (2019). Notes on the nest architecture and colony composition in winter of the yellow-legged Asian hornet, Vespa velutina Lepeletier 1836 (Hym.: Vespidae), in its introduced habitat in Galicia (NW Spain). Insects 10 (8): 237. 10.3390/insects10080237
• Futuyma, D. J., Agrawal, A. A. (2009). Macroevolution and the biological diversity of plants and herbivores. PNAS, 106 (43): 18054-18061. https://doi.org/10.1073/pnas.0904106106
• Ghimire, M. N., Phillips, T. W. (2014). Oviposition and Reproductive Performance of Habrobracon hebetor (Hymenoptera: Braconidae) on Six Different Pyralid Host Species. Annals of the Entomological Society of America, 107 (4): 809-817. https://doi.org/10.1603/AN14046
• Gninenko, Y. I., Shiryaeva, N.V., Shurov, V. I. (2014). The box tree moth - a new invasive pest in the Caucasian forests. Plant Health Research and Practice, 36 (7): 32-39.
• Godfray, H. C. J. (1987). The evolution of clutch size in parasitic wasps. American Naturalist, 129 (2): 221-223.
• Godfray, H. C. J. (1994). Parasitoids: Behavioral and evolutionary ecology. Princeton University Press, NJ, 473 pp.
• Göktürk, T. (2017). The Effect of Dipel and Spruzit Against Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae). International Forestry and Environment Symposium Climate Change and Tree Migration (pp. 261) (7-10 November 2017, Trabzon, Turkey).
• Göttig, S., Herz, A. (2016). Are egg parasitoids of the genus Trichogramma (Hymenoptera: Trichogrammatidae) promising biological control agents for regulating the invasive Box tree pyralid, Cydalima perspectalis (Lepidoptera: Crambidae)?. Biocontrol Science and Technology, 26: 1471-1488. https://doi.org/10.1080/09583157.2016.1211990
• Hardy, I. C., Griffith, N. T., Godfray, H. C. J. (1992). Clutch size in a parasitoid wasp- a manipulation experiment. Journal of Animal Ecology, 61 (1): 121-129. https://doi.org/10.2307/5515
• Harvey, J. A. (2000). Dynamic effects of parasitism by an endoparasitoid wasp on the development of two host species: implications for host quality and parasitoid fitness. Ecological Entomology, 25 (3): 267–278. https://doi.org/10.1046/j.1365-2311.2000.00265.x
• Harvey, J. A., Bezmer, T. M., Elzinga, J.A., Strand, M. R. (2004). Development of solitary endoparasitoid, Microplitis demolitor: host quality does not increase with host age and size. Ecological Entomology, 29 (1): 35–43. https://doi.org/10.1046/j.1365-2311.2000.00265.x
• Herz, A. (2013). 1000 species for biological control - how to protect, enhance and use the diversity of beneficial organisms. Julius-Kühn-Archiv 54 (436): 54-61.
• Hızal, E. (2012). Two Invasive Alien Insect Species, Leptoglossus occidentalis (Heteroptera: Coreidae) and Cydalima perspectalis (Lepidoptera: Crambidae), and their Distribution and Host Plants in Istanbul Province, Turkey. Florida Entomologist, 95 (2), 344-350.
• John, R., Schumacher, J. (2013). Der Buchsbaum-Zünsler (Cydalima perspectalis) im Grenzach Wyhlener Buchswald - Invasionschronik und Monitoringergebnisse. (Der Buchsbaum-Zünsler (Cydalima perspectalis) im Grenzach-Wyhlener Buchswald - Invasionschronik und Monitoringergebnisse.) Gesunde Pflanzen, 65: 1-6.
• Kaplan, I., Carrillo, J., Garvey, M., Ode, P. J. (2016). Indirect plant-parasitoid interactions mediated by changes in herbivore physiology. Current Opinion in Insect Science, 14: 112-119.
• Kenis M., Nacambo S., Leuthardt F. L.G., Domenico F. D., Haye, T. (2013). The box tree moth, Cydalima perspectalis, in Europe: horticultural pest or environmental disaster?. Aliens: The Invasive Species Bulletin, (33):38-41.
• Kester, K. M., Barbosa, P. (1991). Postemergence learning in the insect parasitoid, Cotesia congregata (Say) (Hymenoptera: Braconidae). Journal of Insect Behaviour, 4: 727–742. https://doi.org/10.1007/BF01052227
• Khodzhaev, B. U., Sharafutdinova, S. M., Dzhabbarov, A., Yunusov, S. Y. (1983). Alkaloids of Buxus sempervirens. Chemistry of Natural Compounds, 19: 512–513.
• Khodzhaev, B. U., Primukhamedov, I. M., Nakaidze, N. Y., Yunusov, S. Y. (1984). Alkaloids of Buxus sempervirens. Chemistry of Natural Compounds, 20 (6): 763–764.
• Leuthardt, L. G. F., Billen, W., Baur, B. (2010). Ausbreitung des Buchsbaumzünslers Diaphania perspectalis (Lepidoptera, Pyralidae) in der Region Baseleine für die Schweizneue Schädlingsart. Entomo Helvetica, 3: 51-57.
• Leuthardt, F. L. G., Baur, B. (2013). Oviposition preference and larval development of the invasive moth Cydalima perspectalis on five European Box-tree varieties. Journal Applied Entomology, 137 (6): 437-444. https://doi.org/10.1111/jen.12013
• Leuthardt, F. L.G., Glauser, G., Baur, B. (2013). Composition of alkaloids in different box tree varieties and their uptake by the box tree moth Cydalima perspectalis. Chemoecology, 23: 203–212. https://doi.org/10.1007/s00049-013-0134-1
• López, C., Eizaguirre, M. (2019). Diapause and biological cycle of Cydalima perspectalis (Walker) in the eastern Pyrenees. Journal of Applied Entomology, 143(10): 1096-1104.
• Martin, J. C., Buradino, M., Brinquin, A. S., Correard, M., Thevenet, J., Vauthier, D., Morel, E., Gilli, A, Venard, M., Tabone, E. (2018). Reguler la pyrale du buis Cydalima perspectalis: Limites d’utilisation de la pheromone sexuelle de synthese et premiere approche non scientifique de lapredation par les mesanges (Parus spp.) Scientific Symposium on Boxwood Pests, Tours, France.
• Martini, A., Di Vitantonio C., Dindo, M. L. (2019). Acceptance and suitability of the box tree moth Cydalima perspectalis as host for the tachinid parasitoid Exorista larvarum. Bulletin of Insectology, 72 (1):150-160.
• Nacambo, S., Leuthardt, F. L. G., Wan. H., Li, H., Haye, T., Baur, B., Kenis, M. (2014). Development characteristics of the boxtree moth Cydalima perspectalis and its potential distribution in Europe. Journal of Applied Entomology, 138 (1-2): 14-26. https://doi.org/10.1111/jen.12078
• Nishida, R. (2002). Sequestration of defensive substances from plants by Lepidoptera. Annual Review Entomology, 47: 57-92. 10.1146/annurev.ento.47.091201.145121
• Ode, P. J. (2006). Plant chemistry and natural enemy fitness: effects on herbivore and natural enemy interactions. Annual Review Entomology, 51: 163-185. 10.1146/annurev.ento.51.110104.151110
• Öztürk, N., Akbulut, S., Yüksel, B. (2016). Düzce için yeni bir zararlı Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae). Düzce Üni Ormancılık Dergisi 12 (1), 112-121
• Reudler, J. H., van Nouhuys, S. (2018). The roles of foraging environment, host species, and host diet for a generalist pupal parasitoid. Entomologia Experimentalis et Applicata, 166 (4):251-264. https://doi.org/10.1111/eea.12657
• Saba, E., Shafeeq, T., Irfan, M., Lee, Y. Y., Kwon, H.W., Seo, M. G., Park S. J., Lee, K. Y., Rhee, M. H. (2017). Anti-Inflammatory Activity of Crude Venom Isolated from Parasitoid Wasp, Bracon hebetor Say. Hindawi Mediators Inflamm, volume 2017, Article ID 6978194, 11 pages, https://doi.org/10.1155/2017/6978194
• Salioğlu, Ş. (2020). Artvin ili şimşir alanlarında zarar yapan Cydalima perspectalis (Walker, 1859) (şimşir kelebeği)'in morfolojisi, biyolojisi, zararı ve mücadele olanaklarının araştırılması. Yüksek lisans tezi, Artvin Üniversitesi, Fen Bilimleri Enstitüsü
• Shi, H. Z., Hu, K. F. (2007). Occurrence regularity and control techniques of Diaphania perspectalis (Walker). Hubei Agricultural Sciences, 46: 76-78.
• Strachinis, I., Kazilas, C., Karamaouna, F., Papanikolaou, N. E., Partsinevelos, G. K., Milonas, P. G. (2015). First record of Cydalima perspectalis (Walker, 1859) (Lepidoptera: Crambidae) in Greece. Hellenic Plant Protection Journal, 8 (2): 66-72. https://doi.org/10.1515/hppj-2015-0010
• Tabone, E., Enriquez, T., Venard, M., Colombel, E., Gutleben, C., Guérin, M., Martin, J. C. (2015). Development of a biocontrol program against the box tree moth Cydalima perspectalis (Walker, 1859). IUFRO WP Population Dynamics and integrated control of forest defoliating and other insects. (pp 83) Sopot, Poland.
• Taylor, A. (1988). Host effects on functional and ovipositional responses of Bracon hebetor. Journal of Animal Ecology 57 (1): 173–184. https://doi.org/10.2307/4771
• Toper Kaygın, A., Taşdeler, C. (2019). Cydalima perspectalis (Walker) (Lepidoptera: Crambidae, Spilomelinae)’in Türkiye’de Coğrafi Yayılışı, Yaşam Döngüsü ve Zararı. Bartın Orman Fakültesi Dergisi, 21 (3): 833-847
• Tunca, H. (2010). Bazı Bitkisel Kökenli Ekstrakt ve İnsektisitlerin Chelonus oculator Panzer (Hymenoptera:Braconidae), Bracon hebetor Say (Hymenoptera:Braconidae), Venturia canescens Grav. (Hymenoptera:Ichneumonidae) Gelişimi ve Parazitleme Davranışları Üzerine Etkileri. Ankara Üniversitesi, Fen Bilimleri Enstitüsü, s 110.
• Ullyett, G. C. (1945). Distribtion of progeny by Microbracon hebetor Say. Journal of the Entomological Society of Southern Africa, 8: 123–131.
• Van der Straten, M.J., Muus, T. S. T. (2010). The box tree pyralid, Glyphodes perspectalis (Lepidoptera: Crambidae), an invasive alien moth ruining box trees. Proceedings of the Netherlands Entomological Society Meeting, 21: 107-111.
• Van Emden, H. F. (1995). Host plant–aphidophaga interactions. Agriculture, Ecosystems & Environment, 52: 3–11. https://doi.org/10.1016/0167-8809(94)09001-N
• Vinson, S. B., Iwantsch G. F. (1980). Host suitability for insect parasitoids. Annual Review Entomology, 25: 397–419.
• Wan, H., Haye, T., Kenis, M., Nacambo, S., Xu, H., Zhang, F., Li, H. (2014). Biology and natural enemies of Cydalima perspectalis in Asia: Is there biological control potential in Europe?. Journal Applied Entomology, 138 (10): 715-722. https://doi.org/10.1111/jen.12132.
• Zar, J. H. (1999). Biostatistical Analysis. (4th Edn) Upper Saddle River, NJ: Prentice-Hall.
• Zaviezo, T., Mills, N. (2000). Factors influencing the evolution in clutch size in a gregarious insect parasitoid. Journal Animal Ecololgy, 69 (6): 1047–1057.
• Zımmermann, O., Wührer, B. (2010). Laboruntersuchungen zur Qualitätskontrolle der Larval parasitoide Habrobracon brevicornis und Habrobracon hebetor (Hym.: Braconidae) als Nützlinge in der biologischen Schädlingsbekämpfung. Julius Kühn-Archiv, 428: 149-150.