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New approaches to the control of Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae)

Yıl 2024, , 192 - 200, 21.06.2024
https://doi.org/10.29050/harranziraat.1407990

Öz

Neodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae) is a major pest on Pinus spp. in Europe. In this study, new control methods were tried to control N. sertifer. The effectiveness of Bauveria bassiana (Nostalgist, Bb %1.5 1 x 10 8 kob /ml min, 250 ml/100L), azadirachtin (Nimbecidine, 0.3g azadirachtin/l, 500 ml/da-100L; Nimiks4.5, 40g azadirachtin /l, 200 ml/100L; Neem Azal ®-T/S, 10 g azadirachtin /l, 300ml/100L) and spinosad (Oread, 480 g spinosad/l, 10ml/100L) was tested on N. sertifer larvae. In addition, the effect of the idiobiont parasitoid Bracon hebetor (Say) (Hymenoptera: Braconidae) venom was evaluated. Bauveria bassiana, spinosad and azadirachtin were applied to the larvae by spraying method. Different numbers of B. hebetor females (1-5 females) were also tested on N. sertifer larvae. All experiments were carried out at 25 ± 1°C, 60-70% relative humidity and 16L:8D h photoperiod. After B. bassiana application, the survival rates were 71.66% and 61.66% at the 6th day and 7th day. The maximum efficacy was obtained with azadirachtin in Nimiks 4.5 (40 g azadirachtin/l). The highest mortality (100.0%) was found at 24 h after the application of spinosad (10ml/100l). The most effective combination of B. hebetor female venom on N. sertifer larvae is 5 females and 7 days duration. The study shows that spinosad, azadirachtin and B. hebetor female venom can achieve effective control of N. sertifer larvae.

Kaynakça

  • Abedi, Z., Saber, M., Gharekhani, G., Mehrvar, A., Kamita, S.G., 2014. Lethal and Sublethal Effects of Azadirachtin and Cypermethrin on Habrobracon hebetor (Hymenoptera: Braconidae). Journal of Economic Entomology, 107(2):638-45. doi: 10.1603/EC13227.
  • Adnan, S., Uddin, M., Alam, M., Islam, M., Kashem, M., Rafii, M, et al., 2014. Management of mango hopper, Idioscopus clypealis, using chemical insecticides and neem oil. Science World Journal, 709614. doi: 10.1155/2014/709614
  • Aksu, Y., 2010. Ağaçlandırma Sahalarında Pinus sylvestris’lerde Önemli Zararlar Yapan Neodiprion sertifer (Geoff.) (Hymenoptera; Diprionidae) Üzerine Yapılan Araştırma. Çevre ve Orman Bakanlığı, Orman Genel Müdürlüğü Orman Koruma ve Yangınla Mücadele Dairesi Başkanlığı Yayınları, Ankara
  • Anastas, P., Kirchchoff, M., Williamson, T., 1999. Spinosad a new natural product for insect control. Green Chemistry Award, http://pubs.rsc.org/en/content/articlepdf/1999/GC/A906794I
  • Baş, R., 1973. Türkiye’de Orman Ağaçlarına Zarar Yapan Zar Kanatlılar (Hymenoptera) Üzerine Araştırmalar. Orman Genel Müdürlüğü, Ankara.
  • Biondi, A., Zappalà, L., Stark, J.D., Desneux, N., 2013. Do Biopesticides Affect the Demographic Traits of a Parasitoid Wasp and Its Biocontrol Services through Sublethal Effects? PLoS ONE 28(9): e76548. https://doi.org/10.1371/journal.pone.0076548
  • Brower, J.H., Smith, L., Vail, P.V., Flinn, P.W., 1996. Biological control In B. Subramanyam, & D.W. Hagstrum (Eds). Integrated Management of Insects in Stored Products. (pp. 223- 286) Marcel Dekker, New York, USA.
  • Chelico, L., Khachatourians, G.G., 2008. Isolation and characterization of nucleotide excision repair deficient mutants of the entomopathogenic fungus, Beauveria bassiana. Journal of Invertebrate Pathology, 98: 93–100. doi: 10.1016/j.jip.2007.10.006
  • Cisneros, J., Goulson, D., 2002. Derwent LC, Penagos DI, Hernandez O, Williams T Toxic effects of spinosad on predatory insects. Biological Control, 23: 156-163. https://doi.org/10.1006/bcon.2001.1000
  • Copping, L.G., Menn, J.J., 2000. Biopesticides: a review of their action, applications and efficacy. Pest Management Science, 56: 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
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  • Cura, M.S., Gençer, N.S., 2019. Side Effects of Azadirachtin on Some Important Beneficial Insects in Laboratory. Journal of Biological & Environmental Sciences, 13(37): 39-47.
  • Edwards, J.P., Bell, H.A., Audsley, N., Marris, G.C., Kirkbride-Smith, A., Bryning, G., Frisco, C., Cusson, M., 2006. The ectoparasitic wasp Eldophus pennicornis (Hymenoptera: Eulophiclae) uses instar-specific endocrine disruption strategies to suppress the development of its host Lacanobia oleracea (Lepidoptera: Noctuidae) Journal of Insect Physiology, 52:1153–1162. doi: 10.1016/j.jinsphys.2006.08.003.
  • Elliott, R., Benjamin, M., Gillott, C., 2007. Laboratory studies of the toxicity of spinosad and deltamethrin to Phyllotreta cruciferae (Coleoptera: Chrysomelidae). Canadian Entomologist, 139: (4) 534-544. https://doi.org/10.4039/n06-070.
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  • Ferber, M., Horner, M., Cepok, S., Gnatzy, W., 2001. Digger wasp versus cricket: Mechanisms underlying the total paralysis caused by the predator’s venom. Journal of Neurobiology, 47:207–222. doi: 10.1002/neu.1028.
  • Gao, Y.P., Luo, M., Wang, X.Y., He, X.Z., Lu, W., Zheng, X.L., 2022. Pathogenicity of Beauveria bassiana PfBb and Immune Responses of a Non-Target Host, Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects, 13: 914. https:// doi.org/10.3390/insects13100914.
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  • Gnatzy, W., Volknandt, W., 2000. Venom gland of the digger wasp Liris niger: Morphology, ultrastructure, age-related changes and biochemical aspects. Cell and Tissue Research, 302:271–284. https://doi.org/10.1007/s004410000282
  • Gök, S., Güven, Ö., Karaca, İ., 2018. Entomopatojen fungus Beauveria bassiana’nın çam keseböceği (Thaumetopoea wilkinsoni Tams)’nin farklı dönemlerine etkileri. Türkiye Biyolojik Mücadele Dergisi, 9 (1): 7-19.
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Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae)’in mücadelesinde yeni yaklaşımlar

Yıl 2024, , 192 - 200, 21.06.2024
https://doi.org/10.29050/harranziraat.1407990

Öz

Neodiprion sertifer (Geoffroy) (Hymenoptera: Diprionidae) Avrupa’da Pinus spp.‘nin en önemli zararlısı konumundadır. Bu çalışmada N. sertifer’in mücadelesi için yeni mücadele yöntemleri denenmiştir. Bauveria bassiana (Nostalgist, Bb %1.5 1 x 10 8 kob /ml min, 250 ml/100L), azadirachtin (Nimbecidine, 0.3g azadirachtin, 500 ml/da-100L, Nimiks4.5, 40g azadirachtin /l, 200 ml/100L; Neem Azal ®-T/S, 10 g azadirachtin /l,300ml/100L) ve spinosad’ın (Oread, 480 g spinosad/l, 10ml/100L) etkinliği N. sertifer larvaları üzerinde denenmiştir. Ayrıca idiobiont parazitoit Bracon hebetor (Say) (Hymenoptera: Braconidae)‘un venomu da değerlendirilmiştir. Bauveria bassiana, spinosad ve azadirachtin larvalara püskürtme yoluyla uygulanmıştır. Farklı sayıdaki B. hebetor dişisi de (1-5 dişi) N. sertifer üzerinde test edilmiştir. Bütün denemeler 25 ± 1°C, 60-70% orantılı nem ve 16:8 saat aydınlık:karanlık koşullarda gerçekleştirilmiştir. Bauveria bassiana uygulamasından sonra larvaların yaşam oranı 6. ve 7. günde %71.66 ve %61.66 olarak bulunmuştur. Azadirachtinde maksimum etkinlik Nimiks 4.5 (40 g azadirachtin/l)‘de elde edilmiştir. En yüksek ölüm oranı (%100) spinosad (10ml/100l) uygulamasından 24 saat sonra bulunmuştur. B. hebetor dişi venomunun N. sertifer larvaları üzerinde en etkili olduğu kombinasyon 5 dişi ve 7 gün süredir. Bu çalışma spinosad, azadirachtin ve B. hebetor dişi venomunun N. sertifer larvaları üzerinde etkili bir kontrol sağlayabileceğini göstermiştir.

Kaynakça

  • Abedi, Z., Saber, M., Gharekhani, G., Mehrvar, A., Kamita, S.G., 2014. Lethal and Sublethal Effects of Azadirachtin and Cypermethrin on Habrobracon hebetor (Hymenoptera: Braconidae). Journal of Economic Entomology, 107(2):638-45. doi: 10.1603/EC13227.
  • Adnan, S., Uddin, M., Alam, M., Islam, M., Kashem, M., Rafii, M, et al., 2014. Management of mango hopper, Idioscopus clypealis, using chemical insecticides and neem oil. Science World Journal, 709614. doi: 10.1155/2014/709614
  • Aksu, Y., 2010. Ağaçlandırma Sahalarında Pinus sylvestris’lerde Önemli Zararlar Yapan Neodiprion sertifer (Geoff.) (Hymenoptera; Diprionidae) Üzerine Yapılan Araştırma. Çevre ve Orman Bakanlığı, Orman Genel Müdürlüğü Orman Koruma ve Yangınla Mücadele Dairesi Başkanlığı Yayınları, Ankara
  • Anastas, P., Kirchchoff, M., Williamson, T., 1999. Spinosad a new natural product for insect control. Green Chemistry Award, http://pubs.rsc.org/en/content/articlepdf/1999/GC/A906794I
  • Baş, R., 1973. Türkiye’de Orman Ağaçlarına Zarar Yapan Zar Kanatlılar (Hymenoptera) Üzerine Araştırmalar. Orman Genel Müdürlüğü, Ankara.
  • Biondi, A., Zappalà, L., Stark, J.D., Desneux, N., 2013. Do Biopesticides Affect the Demographic Traits of a Parasitoid Wasp and Its Biocontrol Services through Sublethal Effects? PLoS ONE 28(9): e76548. https://doi.org/10.1371/journal.pone.0076548
  • Brower, J.H., Smith, L., Vail, P.V., Flinn, P.W., 1996. Biological control In B. Subramanyam, & D.W. Hagstrum (Eds). Integrated Management of Insects in Stored Products. (pp. 223- 286) Marcel Dekker, New York, USA.
  • Chelico, L., Khachatourians, G.G., 2008. Isolation and characterization of nucleotide excision repair deficient mutants of the entomopathogenic fungus, Beauveria bassiana. Journal of Invertebrate Pathology, 98: 93–100. doi: 10.1016/j.jip.2007.10.006
  • Cisneros, J., Goulson, D., 2002. Derwent LC, Penagos DI, Hernandez O, Williams T Toxic effects of spinosad on predatory insects. Biological Control, 23: 156-163. https://doi.org/10.1006/bcon.2001.1000
  • Copping, L.G., Menn, J.J., 2000. Biopesticides: a review of their action, applications and efficacy. Pest Management Science, 56: 651-676. https://doi.org/10.1002/1526-4998(200008)56:8<651::AID-PS201>3.0.CO;2-U
  • Cunningham, J.C., Entwistle, P.F., 1981. Control of sawflies by baculovirus. In: Burges H.D., editor. Microbial Control of Pests and Plant Diseases 1970–1980. Academic Press, New York,. p. 379–407.
  • Cura, M.S., Gençer, N.S., 2019. Side Effects of Azadirachtin on Some Important Beneficial Insects in Laboratory. Journal of Biological & Environmental Sciences, 13(37): 39-47.
  • Edwards, J.P., Bell, H.A., Audsley, N., Marris, G.C., Kirkbride-Smith, A., Bryning, G., Frisco, C., Cusson, M., 2006. The ectoparasitic wasp Eldophus pennicornis (Hymenoptera: Eulophiclae) uses instar-specific endocrine disruption strategies to suppress the development of its host Lacanobia oleracea (Lepidoptera: Noctuidae) Journal of Insect Physiology, 52:1153–1162. doi: 10.1016/j.jinsphys.2006.08.003.
  • Elliott, R., Benjamin, M., Gillott, C., 2007. Laboratory studies of the toxicity of spinosad and deltamethrin to Phyllotreta cruciferae (Coleoptera: Chrysomelidae). Canadian Entomologist, 139: (4) 534-544. https://doi.org/10.4039/n06-070.
  • Escherich, K., 1913. Neves uber polyaderhrankheiten. Naturut Z Land Forstwirtsch, 11: 86.
  • Ferber, M., Horner, M., Cepok, S., Gnatzy, W., 2001. Digger wasp versus cricket: Mechanisms underlying the total paralysis caused by the predator’s venom. Journal of Neurobiology, 47:207–222. doi: 10.1002/neu.1028.
  • Gao, Y.P., Luo, M., Wang, X.Y., He, X.Z., Lu, W., Zheng, X.L., 2022. Pathogenicity of Beauveria bassiana PfBb and Immune Responses of a Non-Target Host, Spodoptera frugiperda (Lepidoptera: Noctuidae). Insects, 13: 914. https:// doi.org/10.3390/insects13100914.
  • Ghimire, M.N., 2008. Reproductive Performance of the Parasitoid Bracon hebetor Say (Hymenoptera: Braconidae) On Various Host Species of Lepidoptera. Ph D Thesis.
  • Gnatzy, W., Volknandt, W., 2000. Venom gland of the digger wasp Liris niger: Morphology, ultrastructure, age-related changes and biochemical aspects. Cell and Tissue Research, 302:271–284. https://doi.org/10.1007/s004410000282
  • Gök, S., Güven, Ö., Karaca, İ., 2018. Entomopatojen fungus Beauveria bassiana’nın çam keseböceği (Thaumetopoea wilkinsoni Tams)’nin farklı dönemlerine etkileri. Türkiye Biyolojik Mücadele Dergisi, 9 (1): 7-19.
  • Gökturk, T., Tozlu, G., 2019. The Effect of Pyrethrum and Bacillus thuringiensis Biopesticides on Diprion pini L. and Neodiprion sertifer (Geoffr.) (Hymenoptera: Diprionidae) Larvae. Pakistan Journal of Zoology., 51(3): 989-994. https://doi.org/10.17582/journal.pjz/2019.51.3.989.994
  • Griffiths, K.J., Cunningham, J.C., Otvos, I.S., 1984. Neodiprion sertifer (Geoffroy), European pine sawfly (Hymenoptera: Diprionidae). In: Kelleher JS, Hulme MA, editors. Biological control programmes against insects and weeds in Canada 1969-1980. Slough, UK: Commonwealth Agricultural Bureau, 1984, p. 331-340.
  • Hara, A.H., 2000. Finding alternative ways to control alien pests - Part 2: New insecticides introduced to fight old pests. Hawaii Landscape, 4 (1): 5.
  • Hagstrum, D.W., Smith, B.J.,1997. Host finding ability of Bracon hebetor and its influence upon adult parasite survival and fecundity. Environmental Entomology, 6: 437-439. https://doi.org/10.1093/ee/6.3.437
  • Horowitz, A.R, Ishaaya I.,2004. Biorational Insecticides – Mechanisms, Selectivity and Importance in Pest Management Programs. In: A.R. Horowitz, & I. Ishaaya (Eds.) Insect Pest Management- Field and Protected Crops (pp.1-28) Springer, Berlin, Heidelberg, New York.
  • Isman, M.B., 2006. Botanical insecticides, deterrents and repellents in modern agriculture and an increasingly regulated world. Annual Review Entomology, 51 (1): 45-66. DOI: https://doi.org/10.1146/annurev.ento.51.110104.151146
  • Joseph, S.V., 2020. Repellent effects of insecticides on Stephanitis pyrioides Scott (Hemiptera: Tingidae) under laboratory conditions. Crop Protection., 127: 104985. https://doi.org/10.1016/j.cropro.2019.104985
  • Kandil, C., 2022. Researchs on the Effect of Some Biorational Preparations on the Biological Characteristics of Trichogramma dendrolimi (Matsumura) (Hymenoptera: Trichogrammatidae) Ankara University, Graduate School of Natural and Applied Sciences, Department of Plant Protection, Master Thesis.
  • Kapoor, B., Sharma, K., 2020. Biorational Pesticides: An Envirosafe Alternative to Pest Control. Indian Farmer, 7(08): 722-731.
  • Kilani-Morakchi, S., Morakchi-Goudjil, H., Sifi, K., 2021. Azadirachtin-Based Insecticide: Overview, Risk Assessments, and Future Directions. Frontiers in Agronomy, 3:676208. https://doi.org/10.3389/fagro.2021.676208.
  • Kowalska, J., 2008. Laboratory Studies of the Activity of Spinosad against Leptinotarsa decemlineata (Say) Depending on Different Temperature. 16th IFOAM Organic World Congress, Modena, Italy, June 16-20.
  • Kraiss, H., Cullen, E.M., 2008. Insect growth regulator effects of azadirachtin and neem oil on survivorship, development and fecundity of Aphis glycines (Homoptera: Aphididae) and its predator, Harmonia axyridis (Coleoptera: Coccinellidae). Pest Management Science, 64, 660–668. doi: 10.1002/ps.1541
  • Kryukova, N., Dubovskiy, I., Chertkova, E., Vorontsova, Y., Slepneva, I., Glupov, V., 2011. The effect of Habrobracon hebetor venom on the activity of the prophenoloxidase system, the generation of reactive oxygen species and encapsulation in the haemolymph of Galleria mellonella larvae. Journal of Insect Physiology, 57:769–800. https://doi.org/10.1016/j.jinsphys.2011.03.008
  • Labbé, R.M., Gillespie, D.R., Cloutier, C., Brodeur, J., 2009. Compatibility of an entomopathogenic fungus with a predator and a parasitoid in the biological control of greenhouse whitefly. Biocontrol Science Technology, 19: 429–446. https://doi.org/10.1080/09583150902803229.
  • Larsson, S., Tenow, O., 1984. Areal distribution of a Neodiprion sertifer (Hym, Diprionidae) outbreak on Scots pine as related to stand condition. Ecography, 7 (2):81-90. https://doi.org/10.1111/j.1600-0587.1984.tb01108.x
  • Lin, S., Li, S., Liu, Z., Zhang, L., Wu, H., Cheng, D., Zhang, Z., 2021. Using Azadirachtin to Transform Spodoptera frugiperda from Pest to Natural Enemy. Toxins, 13:541. https://doi.org/10.3390/ toxins13080541
  • Lyons, L.A., 1964. The European pine sawfly, Neodiprion sertifer (Geoff.)(Hymenoptera: Diprionidae). A review with emphasis on studies in Ontario. Proceedings of the Entomological Society Ontario, 94:5-37.
  • Lyytikainen-Saarenmaa, P., Tomppo, E., 2002. Impact of sawfly defoliation on growth of Scots pine Pinus sylvestris (Pinaceae) and associated economic losses. Bulletin Entomology Resesearch, 92: 137–140. DOI: 10.1079/BER2002154.
  • Maral, H., 2021. Effect of spinosad, azadirachtin and kaolin on Stephanitis pyri (Fabricius, 1775) (Hemiptera: Tingidae) under laboratory conditions. Türkiye Entomoloji Dergisi, 45 (1): 87-97. https://doi.org/10.16970/entoted.770859
  • Martínez-Barrera, O.Y., Toledo, J., Cancino, J., Liedo, P., Gómez, J., Valle-Mora, J., Montoya, P., 2020. Interaction Between Beauveria bassiana (Hypocreales: Cordycipitaceae) and Coptera haywardi (Hymenoptera: Diapriidae) for the Management of Anastrepha obliqua (Diptera: Tephritidae). Journal Insect Science, 20(2):6. doi: 10.1093/jisesa/ieaa010. PMID: 32186740; PMCID: PMC707178
  • Matthews, G.A., 2006. Pesticides: Health, Safety and the Environment. Blackwell Publishing, Oxford, UK
  • Mayes, M.A., Thompson, G.D., Husband, B., Miles, M.M., 2003. Spinosad toxicity to pollinators and associated risk. Environmental Contamination and Toxicology, 179:37-71. doi: 10.1007/0-387-21731-2_2. PMID: 15366583.
  • Moreau, S.J., Asgari, S., 2015. Venom Proteins from Parasitoid Wasps and Their Biological Functions. Toxins, 7(7):2385-412. doi: 10.3390/toxins7072385
  • Nakahara, Y., Shimura, S., Ueno, C., Kanamori, Y., Mita, K., Kiuchi, M., et al., 2009. Purification and characterization of silkworm hemocytes by flow cytometry. Dev. Comp. Immunol. 33: 439–448. doi: 10.1016/j.dci.2008.09.005
  • Naqqash, M.N., Gökçe, A., Bakhsh, A., Salim, M., 2016. Insecticide resistance and its molecular basis in urban insect pests. Parasitology Research,115: 1363–1373. doi: 10.1007/s00436-015-48989
  • Quistad, G.B., Nguyen, Q., Bernasconi, P., Leisy, D.J., 1994. Purification and characterization of insecticidal toxins from venom glands of the parasitic wasp, Bracon hebetor. Insect Biochemistry and Molecular Biology, 24: 955-961. doi: 10.1016/0965-1748(94)90132-5.
  • Podgwaite, J.D., Rush, P., Hall, D., Walton, A.S., 1984. Efficacy of the Neodiprion sertifer (Hymenoptera: Diprionidae) Nucleopolyhedrosis Virus (Baculovirus) Product, Neochek-S. Journal of Economic Entomology, 77(2): 525-528. https://doi.org/10.1093/jee/77.2.525
  • Price, D., Bell, H., Hinchliffe, G., Fitches, E., Weaver, R., Gatehouse, J.A., 2009. venom metalloproteinase from the parasitic wasp Eulophus pennicornis is toxic towards its host, tomato moth (Lacanobia oleracae) Insect Molecular Biology, 18:195–202. doi: 10.1111/j.1365-2583.2009.00864.x.
  • Pschorn-Walcher, H., 1965. The ecology of Neodiprion sertifer (Geoffr.)(Hym.: Diprionidae) and a review of its parasite complex in Europe. Technical Bulletin, Commonwealth Institute of Biological Control, 5:33-97.
  • Pschorn-Walcher, H., 1982. Suborder Symphyta, sawflies. In: Schwenke W, editor. Die Forstschaedlinge Europas. 4. Hautflügler und Zweiflügler. Hamburg, Germany: Paul Parey, 66-129.
  • Şimşek, Z., 2006. Kondur Y. Çankırı Ormanlarının Zararlı Böcekleri ve Mücadele Yöntemleri. Gazi Üniversitesi, Orman Fakültesi Dergisi, 6(1): 105-107.
  • Tian, C., Wang, L., Ye, G., Zhu, S., 2010. Inhibition of melanization by a Nasonia defensin-like peptide: Implications for host immune suppression. Journal Insect Physiology, 56:1857–1862. doi: 10.1016/j.jinsphys.2010.08.004.
  • Tosun, İ., 1975. Akdeniz Bölgesi İğne Yapraklı Ormanlarında Zarar Yapan Böcekler ve Önemli Türlerin Parazit ve Yırtıcıları Üzerinde Araştırmalar. Orman Bakanlığı Orman Genel Müdürlüğü Yayınları, İstanbul.
  • Tunca, H., Kılınçer, N., Özkan, C., 2012. Side-effects of some botanical insecticides and extracts on the parasitoid, Venturia canescens (Grav.) (Hymenoptera: Ichneumonidae). Türkiye Entomoloji Dergisi, 36 (2): 205-214.
  • Tuncsoy, B.S., Bueno-Pallero, F., Dionisio, L., Neto, L., Ozalp, P., 2020. Effect of Beauveria bassiana on Galleria mellonella L. (Lepidoptera: Pyralidae) and Its Parasitoid Trichogramma cacoeciae. Karaelmas Fen ve Müh Dergisi, 10(2):131-136.
  • Virtanen, T., Neuvonen, S., Nikula, A., Varama, M., Niemela, P., 1996. Climate change and the risk of Neodiprion sertifer outbreaks on Scots pine. Silva Fennica, 30: 169–177.
  • Wang, H., Peng, H., Li, W., Cheng, P., Gong, M., 2021. The Toxins of Beauveria bassiana and the Strategies to Improve Their Virulence to Insects. FrontMicrobiology, 12:705343. doi: 10.3389/fmicb.2021.705343
  • Williams, T., Valle, J., Vinuela, E., 2003. Is the naturally derived insecticide spinosad® compatible with insect natural enemies? Biocontrol Science Technology, 13 (5): 459-475. https://doi.org/10.1080/0958315031000140956.
  • Wraight, S.P., Ramos, M.E., Avery, P.B., Jaronski, S.T., 2010. Vandenberg JD. Comparative virulence of Beauveria bassiana isolates against lepidopteran pests of vegetable crops. Journal of Invertebrate Pathology, 103: 186–199. doi: 10.1016/j. jip.2010.01.001
  • Yaman, M., Nalçacıoğlu, R., Demirbağ, Z., 2001. Viral Control of the European Pine Sawfly, Neodiprion sertifer (Geoffroy) in Turkey. Turkish Journal of Biology, 25 419-425.
  • Yiğit, Ş., Saruhan, İ., 2022. Entomopatojenik funguslar; Beauveria bassiana, Isaria fumosorosea ve Metarhizium anisopliae’nın Palomena prasina L. (Heteroptera: Pentatomidae)’ya karşı etkileri. KSÜ Tarım ve Doğa Dergisi, 25 (5): 1051-1060. https://doi.org/10.18016/ksutarimdoga. vi. 938399
  • Zar, J.H., 1999. Biostatistical analysis. – Prentice-Hall, upper Sadle River, NJ,.
Toplam 62 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Bitki Koruma (Diğer)
Bölüm Araştırma Makaleleri
Yazarlar

Hilal Tunca 0000-0003-3073-6628

Damla Çayci 0000-0003-2918-7609

Erken Görünüm Tarihi 19 Haziran 2024
Yayımlanma Tarihi 21 Haziran 2024
Gönderilme Tarihi 21 Aralık 2023
Kabul Tarihi 13 Mayıs 2024
Yayımlandığı Sayı Yıl 2024

Kaynak Göster

APA Tunca, H., & Çayci, D. (2024). New approaches to the control of Neodiprion sertifer (Geoffroy, 1785) (Hymenoptera: Diprionidae). Harran Tarım Ve Gıda Bilimleri Dergisi, 28(2), 192-200. https://doi.org/10.29050/harranziraat.1407990

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