Polymorphism of stearoyl-CoA desaturase (SCD) gene in Hereford cattle breed

Yıl 2019, Cilt: 23 Sayı: 2, 247 - 253, 18.06.2019

Hazniye Nergis Seyrani Koncagül , Selahaddin Kiraz

https://doi.org/10.29050/harranziraat.487490

Öz

In this study, Stearoyl CoA Desaturase (SCD) gene polymorphism was
determined in Hereford cattle breed. A702G, T762C and C878T single nükleotid
polymorfphisms (SNPs) have been identified based on DNA sequence information of
exon V region in SCD gene. AA, AG and GG genotype frequencies were 20.69%,
44.83% and 34.45% in A702G region, and TT, TC and CC genotype frequencies were
13.79%, 62.07% and 24.14% T762C region, and CC, CT and TT genotype frequencies
were %44.83, 31.03 and 24.14
in C878T region, respectively. In addition, A and G
allele frequencies were 0.431 and 0.569 in A702G region, T and C allele
frequencies were 0.448 and 0.552
in T762C region, and C and T allele frequencies were
0.603 and 0.397 in
C878T region, respectively. In addition, it was found that the population was
not at Hardy-Weinberg equilibrium in terms of related SNP genotypes.

Anahtar Kelimeler

Hereford, Stearoyl CoA Desaturase gene, Polymorphisms

Hereford ırkı sığırlarda stearoyl-CoA desaturase (SCD) gen polimorfizmi

Öz

Bu çalışmada,
Hereford ırkı sığır populasyonunda Stearoyl CoA Desaturase (SCD) gen
polimorfizmi belirlenmiştir. SCD geni ekson V bölgesi gen dizi bilgilerine göre
A702G, T762C ve C878T tek nükleotid polimorfizmleri (SNP) tespit edilmiştir.
A702G bölgesinde AA, AG ve GG genotiplerinin frekansları sırasıyla; %20.69,
44.83 ve 34.45, T762C bölgesinde TT, TC ve CC genotiplerinin frekansları
sırasıyla; %13.79, 62.07 ve 24.14, C878T bölgesinde CC, CT ve TT genotiplerinin
frekansları sırasıyla; %44.83, 31.03 ve 24.14 olarak hesaplanmıştır. Bununla
beraber, A702G bölgesinde A ve G allel frekansları 0.431 ve 0.569, T762C
bölgesinde T ve C allel frekansları 0.448 ve 0.552, C878T bölgesinde C ve T
allel frekansları 0.603 ve 0.397'dir. Ayrıca ilgili SNP’ler bakımından
populasyonun Hardy-Weinberg dengesinde olmadığı belirlenmiştir (P>0.05).

Anahtar Kelimeler

Hereford, Stearoyl CoA Desaturase geni, Polimorfizm

Kaynakça

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