Determination of phytochemical contents, antioxidant and antibacterial properties with phenolic compounds of extracts of Molehiya (Corchorus olitorius L.) and Altın otu (Helichrysum arenarium L.)

Yıl 2024, Cilt: 28 Sayı: 1, 131 - 145, 25.03.2024

Filiz Uçan Türkmen , Kibar Yapıcı , Elife Osman , Gülcan Koyuncu , Ümit Haydar Erol

https://doi.org/10.29050/harranziraat.1327041

Öz

n this study, phytochemical contents, antioxidant activities, antimicrobial activities and phenolic components of methanol extracts of Molehiah (Corchorus olitorius L.) and Golden grass (Helichrysum arenarium L.) plants were investigated. When the two plant extracts were compared in terms of total phenolic and flavonoid contents, higher data were obtained in Molehiah extract. The amount of ascorbic acid of golden grass methanol extract was 5 times higher than that of Molehiah methanol extract. While the DPPH radical scavenging activity was determined as 76.97% in Molehiah methanol extract, this value was found to be lower in the methanol extract of golden grass (73.06%). When all antioxidant analyzes were evaluated together, the antioxidant values of Molehiah methanol extract were found to be higher than Goldengrass methanol extract in 4 of 6 antioxidant analyses. The total amount of phenolic components was determined as Golden Herb methanol extract (110.866,394 mg kg-1) > Molehiah methanol extract (39.803,77 mg kg-1). While no inhibitory effect was observed in the extracts against E. coli ATCC 25922, Salmonella typhimurium ATCC 14028 and Pseudomonas aeruginosa ATCC 9027; Inhibitory effect against Staphylococcus aureus ATCC 25923 was observed in methanol extracts of Golden grass and Molehiah, and the zone diameters were measured as 12 and 9 mm, respectively. According to our results, it is possible to say that methanol extracts of these plants can be useful for food, phytotherapy and medical applications.

Anahtar Kelimeler

Molehiah (Corchorus olitorius L.), Golden grass (Helichrysum arenarium L.), Phenolic compund, Antioxidan, Antibacterial

Molehiya (Corchorus olitorius L.) ve Altın otu (Helichrysum arenarium L.) ekstraktlarının fitokimyasal içerikleri, antioksidan ve antibakteriyel özellikleri ile fenolik bileşenlerinin belirlenmesi

Öz

Bu çalışmada, Molehiya (Corchorus olitorius L.) ve Altın otu (Helichrysum arenarium L.) bitkilerinin metanol ekstraktlarının fitokimyasal içerikleri, antioksidan aktiviteleri ve antimikrobiyal aktiviteleri ile fenolik bileşenleri araştırılmıştır. Toplam fenolik ve flavonoid içerikleri bakımından iki bitki ekstraktı kısyaslandığında Molehiya ekstraktında daha yüksek verilere ulaşılmıştır. Altın otu metanol ekstraktının askorbik asit miktarı Molehiya metanol ekstraktından 5 kat fazla bulunmuştur. Molehiya metanol ekstraktında DPPH. radikali giderme aktivitesi %76.97 olarak tespit edilirken, Altın otu metanol ekstraktında ise bu değer daha düşük (%73.06) bulunmuştur. Tüm antioksidan analizleri birlikte değerlendirildiğinde, 6 antioksidan analizinin 4’ünde Molehiya metanol ekstraktının antioksidan değerleri, Altın otu metanol ekstraktından daha yüksek tespit edilmiştir. Fenolik bileşen miktarları toplamı, Altın Otu metanol ekstraktı (110.866,394 mg kg-1) > Molehiya metanol ekstraktı(39.803,77 mg kg-1) şeklinde tespit edilmiştir. Escherichia coli ATCC 25922, Salmonella typhimurium ATCC 14028 ve Pseudomonas aeruginosa ATCC 9027’ye karşı özütlerde herhangi bir inhibitör etkiye rastlanmazken; Staphylococcus aureus ATCC 25923’e karşı inhibitör etki, Altın otu ve Molehiya metanol ekstraktlarında gözlenmiş ve zon çapları sırasıyla 12 ve 9 mm olarak ölçülmüştür. Sonuçlarımıza göre, bu bitkilerin metanol ekstraktlarının gıda, fitoterapi ve tıbbi uygulamalar için yararlı olabileceğini söylememiz mümkündür.

Anahtar Kelimeler

Molehiya (Corchorus olitorius L.), Altın otu (Helichrysum arenarium L.), Fenolik bileşen, Antioksidan, Antibakteriyel

Kaynakça

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