Yetiştirme substratı kompozisyonunun Ganoderma lucidum'un biyolojik verimliliği ve kalite parametreleri üzerine etkileri
Yıl 2024,
Cilt: 28 Sayı: 2, 235 - 247, 21.06.2024
Gülsüm İnce
,
Mustafa Kemal Soylu
,
Nuray Çömlekçioğlu
Öz
Tıbbi bir mantar olan Ganoderma lucidum'un yetiştirilmesi için bazı bitkilerin atık kısımlarını değerlendirmek amacıyla bir substrat bileşimi deneyi gerçekleştirilmiştir. Ganoderma tohumluk miselleri (darıya sardırılmış) Atatürk Bahçe Bitkileri Merkezi Araştırma Enstitüsü'nden (Yalova, Türkiye) sağlanmıştır. Bu çalışmada dokuz (9) farklı substrat reçetesi kullanılmıştır; S1: %40 talaş + %40 nohut sapı + %20 kepek, S2: %60 talaş + %20 nohut sapı + %20 kepek, S3: %40 talaş + %40 bezelye sapı + %20 kepek, S4: %60 talaş + %20 bezelye sapı + %20 kepek, S5: %40 talaş + %40 haşhaş sapı + %20 kepek, S6: %60 talaş + %20 haşhaş sapı + %20 kepek, S7: %40 talaş + %40 mısır koçanı + %20 kepek, S8: %60 talaş + %20 mısır koçanı + %20 kepek ve S9 (Kontrol): %80 talaş + %20 kepek. Tüm substratlardan mantar elde edilmiştir. Biyolojik verimlilik (BE) %7.84 ile %17.92 arasında değişmiştir. S2, S5, S6, S7, S8 ve S9'daki BE, S4, S3 ve S1'den daha yüksek bulunmuştur. En yüksek toplam verim 88.38 g 1.5 kg torba-1 ile S6'da kaydedilmiştir. En erken hasat edilen mantar 51 gün ile mısır koçanı (S8) ve talaş (S9) substratlarından elde edilmiştir. Mantarların protein içeriği %31.51 ile 51.4 arasında değişmiştir. Mantarların nitrojen, potasyum, kalsiyum, magnezyum ve demir içeriği substrata %40 mısır koçanı karıştırılarak arttırılmış, %20 bezelye sapı eklenerek çinko zenginleştirilmiştir. Substrata mısır koçanı eklenmesi, verimi ve biyolojik etkinliği azaltmadan Ganoderma mantarının proteini ve mineral içeriğini zenginleştirmiştir.
Destekleyen Kurum
Atatürk Horticultural Central Research Institute
Proje Numarası
Scientific Research Projects Coordination Unit of Eskisehir Osmangazi University supported this study. Project no: FYL-2022-2274.
Teşekkür
Scientific Research Projects Coordination Unit of Eskisehir Osmangazi University supported this study. Project no: FYL-2022-2274. Special thanks to Atatürk Horticultural Central Research Institute for supplying the Ganoderma lucidum spawn.
Kaynakça
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Effects of cultivation substrate composition on biological productivity and quality parameters of Ganoderma lucidum
Yıl 2024,
Cilt: 28 Sayı: 2, 235 - 247, 21.06.2024
Gülsüm İnce
,
Mustafa Kemal Soylu
,
Nuray Çömlekçioğlu
Öz
A substrate composition experiment was carried out to utilize the waste parts of certain plants for cultivation of Ganoderma lucidum, a medicinal mushroom. Ganoderma mycelium (millet spawn) provided by the Atatürk Horticultural Central Research Institute (Yalova, Türkiye). Nine (9) different substrate recipes were used in this study; S1: 40% sawdust + 40% chickpea stalk + 20% bran, S2: 60% sawdust + 20% chickpea stalk + 20% bran, S3: 40% sawdust + 40% pea stalk + 20% bran, S4: 60% sawdust + 20% pea stalk + 20% bran, S5: 40% sawdust + 40% poppy stalk + 20% bran, S6: 60% sawdust + 20% poppy stalk + 20% bran, S7: 40% sawdust + 40% corn cob + 20% bran, S8: 60% sawdust + 20% corncob + 20% bran and S9 (Control): 80% sawdust + 20% bran. Mushroom fruiting bodies were obtained from all substrates. Biological efficiency (BE) was varied between 7.84 and 17.92%. BE in S2, S5, S6, S7, S8 and S9 was higher than S4, S3 and S1. The highest total yield was recorded in S6 with 88.38 g 1.5 kg bag-1. The earliest mushroom was harvested from corncob (S8) and sawdust (S9) recipes within 51 days. The protein content of fruiting bodies ranged from 31.51% to 51.4%. Nitrogen, potassium, calcium, magnesium and iron content of fruiting bodies were increased by mixing 40% corncob to substrate and zinc was enriched by adding 20% chick pea stalk. Adding corncob to the substrate may enrich the Ganoderma fruiting body protein and mineral content without decreasing the yield and biological efficiency.
Proje Numarası
Scientific Research Projects Coordination Unit of Eskisehir Osmangazi University supported this study. Project no: FYL-2022-2274.
Kaynakça
- Aghajani, H., Bahmani, M., Tajick Ghanbary, M. A., Zahedian, E., 2018. Influence of relative humidity and temperature on cultivation of Pleurotus species. Maderas. Ciencia y tecnologia, 20(4) 571-578. Universidad del Bio-Bio.
- A.O.A.C. (2019). Official Methods of Analysis of AOAC International [www Document]. 21stEd.https://www.aoac.org/aoac_prod_imis/AOAC/Publications/Official_Methods_of_Analysis/AOAC_Member/Pubs/OMA/AOAC_Official_Methods_of_Analysis.aspx
- Açıkgöz, N., İlker, E., & Gökçöl, A. (2004). Biyolojik araştırmaların bilgisayarda değerlendirilmeleri, Ege Üniversitesi Tohum Teknolojisi Uyg. ve Araş. Merkezi yayınları No:2, Ege Üniversitesi Ziraat Fakültesi Ofset Atölyesi, Bornova-İzmir, 202s.
- Adongbede, E.M., & Atoyebi, A.F. (2021). Isolation of Ganoderma lucidum (Curtis) P. Karst. From the wild in Lagos through tissue culture techniques and cultivation on sawdust of six Nigerian hardwoods. Jasem 25 (8), 1329–1335. https://doi.org/10.4314/jasem.v25i7.32
- Ahmad, R., Riaz, M., Khan, A., Aljamea, A., Algheryafi, M., Sewaket, D., & Alqathama, A. (2021). Ganoderma lucidum (Reishi) an edible mushroom; a comprehensive and critical review of its nutritional, cosmeceutical, mycochemical, pharmacological, clinical, and toxicological properties. Phytotherapy Research, 35(11), 6030–6062. https://doi.org/10.1002/ptr.7215
- Amiri-Sadeghan, A., Aftabi, Y., Arvanagh, R.A., Shokri, E., Khalili, M., Seyedrezazadeh, E. & Kuhar, F. (2022). A Review of substrates for solid-state fermentation of lingzhi or reishi medicinal mushroom, Ganoderma lucidum (Agaricomycetes), for basidiome production and effect on bioactive compounds. International Journal of Medicinal Mushrooms, 24(4): 15-29.
- Atila, F. (2020). Comparative study on the mycelial growth and yield of Ganoderma lucidum (Curt.: Fr.) Karst. Acta Ecologica Sinica, 40:153-157. https://doi.org/10.1016/j.chnaes.2018.11.007
- Atila, F. (2022). Utilization of agricultural and forestry by-products in Ganoderma lucidum (Curt.: Fr.) P. Karst production. The Journal of Fungus. 13 (Special issue)1-8, 3rd International Eurasian Mycology Congress.
- Atoji-Henrique, K., Henrique, D.S., Gloria, L.S., Mazaro, S.M., & Casagrande, M. (2017). Influence of substrate composition on beta-glucans production and growth of Ganoderma lucidum. The Journal of Agricultural Science, 9, 190.
- Aydoğdu, M., & Kurbetli, İ. (2021). Biotic interactions of cultivated mushroom and green mold disease in compost and casing soil. Harran Tarım ve Gıda Bilimleri Dergisi, 25(1), 13-22. https://doi.org/10.29050/harranziraat.795177
- Baktemur, G., Kara E., Yarar M., Soylu, M.K., & Taşkın, H. (2022). Use of different agricultural wastes in Ganoderma carnosum Pat cultivation. Turkish Journal of Agriculture and Forestry: Vol. 46: No. 3, Article 8.
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