Araştırma Makalesi
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Effect of Ensiling Time on Microbial Composition and Aerobic Stability of Total Mixture Ration

Yıl 2024, Cilt: 65 Sayı: 2, 119 - 129, 31.12.2024
https://doi.org/10.29185/hayuretim.1532368

Öz

Objective: In this study, the effects of ensiling total mixed ration (TMR) on aerobic stability properties were investigated.
Material and Methods: In the study, half of the TMR with 55% DM content was fresh and the other half was subjected to aerobic stability test after being siled for 30, 60, 90 days. The analyses of chemical and microbiological parameters in feed samples were carried out at 0, 12, 24, 48, 72 and 96 hours of aerobic stability. At the same time, making the T200 IR imaging brand evaluation results in each treatment group at 1 m from the silage samples were recorded with a thermal imaging camera.
Results: In the study, crude protein (CP), crude ash (CA), ether extract (EE), starch, neutral detergent fiber (NDF) and acid detergent (ADF) values of TMR decreased as the ensiling time increased (P<0.001). The pH, dry matter (DM), lactic acid (LA) values decreased but NH3-N and NH3-N/TN values increased with the duration of aerobic stability. Ensiling had positive effects on microbial composition and yeast and mould values decreased (P<0.000).
Conclusion: As a result of this research, although 55% KM TRK is best consumed fresh, ensiling is better in terms of aerobic stability.
Key Words: Aerobic stability, ensiling time, temperature sensor, thermal camera, total mixed ration

Kaynakça

  • Addah W, Baah J, Okine EK, McAllister T A. 2012. Use of thermal imaging and the in situ technique to assess the impact of an inoculant with feruloyl esterase activity on the aerobic stability and digestibility of barley silage. Canadian Journal of Animal Science, 92(3), 381-394.
  • Adesogan AT, Salawu MB. 2004. Effect of applying formic acid, heterolactic bacteria or homolactic and heterolactic bacteria on the fermentation of bi-crops of peas and wheat. Journal of Science of Food and Agriculture 84: 983-992.
  • Agma Okur A, Gozluklu K, Okur E, Okuyucu B, Koc F, Ozduven ML. 2022. Effects of apple vinegar addition on aerobic deterioration of fermented high moisture maize using ınfrared thermography as an indicator. Sensors, 22(3):771.
  • Anonymous 1986. The Analysis of Agricultural Material, Reference Book: 427. London, Pp. 428.
  • AOAC. 1990. In K. Helrich (Ed.), Official Methods of Analysis (15th ed.). Arlington, VA, USA: Association of Official Analytical Chemists, Inc.
  • Ashbell G, Weinberg ZG, Hen Y, Filya I. 2002. The effects of temperature on the aerobic stability of wheat and corn silages. J. Ind. Microbiol. Biotechnol. 28, 261–263.
  • Berk Z. 2018. Physical Properties of Food Materials. ln: Food Process Engineering and Technology. A volume in Food Science and Technology. Elsevier lnc. pp. 7-25.Available at: https://doi.org/10.1016/B978-0-12-373660-4.00001-6 (Cited 13 April 2020).
  • Coşkuntuna L, Erten K, Koç F. 2022. Toplam rasyon karışımının silolanmasının aerobik stabilite özellikleri üzerine etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 9(4), 933-942.
  • Driehuis F, Oude Elferink SJWH. 2000. The impact of the quality of silage on animal health and food safety: A review. Veterinary Quarterly, 22: 212-216.
  • Dubois M, Giles KA, Hamilton JK, Rebes PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356.
  • Heikkila T, Saarisalo E, Taimisto AM, Jaakkola S. 2010. Effects of dry matter and additive on wilted bale silage quality and milk production. ln: Schnyder, H. et al. (Eds.) Grassland in a changing world: proceedings of the 23th General Meeting of the European Grassland Federation, Kiel, Germany August 29th - September 2nd 2010. Grassland Science in Europe 15: pp. 500-502.
  • Holmes BJ, Bolsen KK. 2009. What's new in silage management. ln: Broderick,
  • Jaakkola S, Saarisalo E, Heikkila T. 2010. Aerobic stability and fermentation quality of round bale silage treated with inoculants or propionic acid. Schnyder, H. et al. (Eds.) Grassland in a changing world: Proceedings of the 23th General Meeting of the European Grassland Federation, Kiel, Germany August 29th - September 2nd 2010. Grassland Science in Europe 15: 503-504.
  • Koç F, Özdüven ML, Demirci AŞ, Şamlı HE. 2018. Mısır silajlarında saha şartlarında aerobik stabilite süresince mikrobiyal kompozisyondaki değişikliklerin termal kamera görüntüleme tekniği ile değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi. 21 (2): 167-174
  • Koivunen E, Huuskonen A. 2018. Sailörehun hometoksiinit ja niiden vaikutukset nau- doilla. Luonnonvara- ja biotalouden tutkimus 19/2018. Luonnonvarakeskus, Helsinki, Finland. pp. 24. Available at: http://urn.fi/URN:lSBN:978-952-326-562-2 (Cited 24 November 2019). Kung L. Jr. 2005. Aerobic Stability of Silages. Proc. of the Conference on Silage for Dairy Farms. Harrisburg. Available at: https://www.academia.edu/6566050/Aerobic_Stabil-ity_of_Silages (Cited 20 October 2019).
  • Muck RE, Moser LE, Pitt RE. 2003. Postharvest factors affecting ensiling. ln: Bux- ton, D.R., Muck, R.E. & Harrison, J.H., (Eds.) Silage Science and Technology. Agronomy Publication No 42, American Society of Agronomy, Madison, Wisconsin USA. pp. 251-304.
  • Nishino N, Hattori H. 2007. Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria. Journal of the Science of Food and Agriculture, 87(13), 2420-2426.
  • Ogunade M, Martinez-Tuppia C, Queiroz OCM, Jiang Y, Drouin P, Wu F, Vyas D, Adesogan AT. 2018. Silage review: Mycotoxins in silage: Occurrence, effects, prevention, and mitigation. Journal of Dairy Science 101: 4034-4059.
  • Pahlow G, Muck RE, Driehuis F, Elferink SJO, Spoelstra, SF. 2003. Microbiology of ensiling. Silage Science and Technology, 42, 31-93.
  • Pauly T, Wyss U. 2018. Methodology of ensiling trials and effects of silage additives. ln: Gerlach, K. & Südekum, K.-H. (Eds.). Proceedings of the XVlll lnternational Si- lage Conference, 24-26-July 2018, Bonn, Germany. p.196-209.
  • Pursiainen P, Tuori M. 2008. Effect of ensiling field bean, field pea and common vetch in different proportions with whole-crop wheat using formic acid or an inoculant on fermentation characteristics. Grass and Forage Science 63: 60-78.
  • Ranjit NK, Kung Jr L. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 83, 526–535.
  • Rinne M, Franco M, Kuoppala K, Seppala A, Jalava T. 2018. Response to total mixed ration stabilizers depends on feed quality. ln: Gerlach, K. & Südekum, K.-H. (Eds.). Proceedings of the XVlll lnternational Silage Conference, 24-26 July 2018, Bonn, Germany. p.538-539.
  • Rose D, Bianchini A, Martinez B, Flores R. 2012. Methods for reducing microbial contamination of wheat flour and effects on functionality. Cereal Foods World 57: 104-109.
  • Saarisalo E, Jalava T, Skytta E, Haikara A, Jaakkola S. 2006. Effects of dry matter and additive on wilted bale silage quality and milk production. Agricultural and Food Science 15: 185-199.
  • Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF, 1990. Methods for the microbiological analysis of silage. Grovfoder (Sweden).
  • Seppala A, Maki M, Orkola S, Rinne M. 2015. Aerobic stability of crimped barley ensiled with organic acids. ln: Uden, P. (Ed.) Proceedings of the 6th Nordic feed sci- ence conference, Uppsala, Sweden, 4-5 June, 2015. Swedish University of Agricul- tural Sciences. Department of Animal Nutrition and Management. Report 291: 71- 76.
  • Shah A.A, Qian C, Liu Z, Wu J, Sultana N, Mobashar M, Wanapat M, Zhong X 2021. Evaluation of biological and chemical additives on microbial community, fermentation characteristics, aerobic stability, and in vitro gas production of SuMu No. 2 elephant grass. J. Sci. Food Agric.101, 5429–5436.
  • Soycan Önenç S, Korkmaz Turgud F, Turan Uçman A. 2019. Kekik ve kimyon uçucu yağlarının yonca silajlarının fermantasyon kalitesi, aerobik stabilite ile yem değeri üzerine etkileri. Hayvansal Üretim, 60(2), 117-123.
  • Soysal Mİ. 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları), Yayın No: 95, Ders Kitabı No: 64, T. Ü. Tekirdağ Ziraat Fakültesi Tekirdağ.
  • Statistics for the Windows Operating System 1999. Stat Soft Inc., Tulsa, OK, USA.
  • Türkgeldi B, Koç F, Lackner M, Okuyucu B, Okur E, Palangi V, Esen S. 2023. Infrared thermography assessment of aerobic stability of a total mixed ration: An innovative approach to evaluating dairy cow feed. Animals, 13(13):2225.
  • Ünal Ö, Koç F, Okur AA, Okur E, Özdüven ML. 2018. Mısır ve buğday silajlarının termal kamera görüntüleme tekniği kullanılarak aerobik stabilitesinin değerlendirilmesi. Alınteri Journal of Agriculture Sciences, 33(1): 55-63.
  • Van Soest PV, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597.
  • Wang F, Nishino N. 2008. Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability. Journal of the Science of Food and Agriculture, 88(1), 133-140.
  • Weinberg ZG, Chen Y, Miron D, Raviv Y, Nahim E, Bloch A, Miron J. 2011. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film–A commercial scale experiment. Animal Feed Science and Technology, 164(1-2), 125-129.
  • Weiss K, Kroschewski B, Auerbach H. 2016. Effects of air exposure, temperature and additives on fermentation characteristics, yeast count, aerobic stability and volatile organic compounds in corn silage, Journal of Dairy Science 10: 8053-8069.
  • Wilkinson J M, Davies DR. 2013. The aerobic stability of silage: key findings and recent developments. Grass and Forage Science, 68, 1–19.
  • Xie Y, Wang L, Li W, Xu S, Bao J, Deng J, Wu Z, Yu Z. 2022. Fermentation quality, In vitro digestibility, and aerobic stability of total mixed ration silage in response to varying proportion alfalfa silage. Animals. 2022; 12(8):1039.

Silolama Süresinin Toplam Rasyon Karışımının Mikrobiyal Kompozisyonu ve Aerobik Stabilitesi Üzerine Etkisi

Yıl 2024, Cilt: 65 Sayı: 2, 119 - 129, 31.12.2024
https://doi.org/10.29185/hayuretim.1532368

Öz

Amaç: Bu araştırmada, toplam rasyon karışımı (TRK)’nın silolanmasının aerobik stabilite özellikleri üzerine etkileri araştırılmıştır.
Materyal ve Metot: Araştırmada %55 KM içeriğine sahip TRK’nın yarısı taze olarak, diğer yarısı 30, 60, 90 gün silolandıktan sonra, aerobik stabilite testine tabi tutulmuşlardır. Aerobik stabilitenin 0., 12., 24., 48., 72. ve 96. saatlerinde yem örneklerinde kimyasal ve mikrobiyolojik parametrelere ilişkin analizler yürütülmüştür. Aynı zamanda, T200 IR marka termal kamera ile 1 m mesafeden silaj örneklerinde her muamele grubunda görüntüleme yapılarak değerlendirme sonuçları kaydedilmiştir. Daha sonra elde edilen veriler ThermaCAM software programında değerlendirilmiştir.
Bulgular: Araştırmada silolama süresi artıkça TRK’nın ham protein (HP), ham kül (HK), ham yağ (HY), nişasta, Nötr deterjanda çözünmeyen lif (NDF), Asit deterjanda çözünmeyen lif (ADF) değerleri düşmüştür (P<0.001). Aerobik stabilite süresine bağlı olarak pH, kuru madde (KM), laktik asit (LA) değerleri düşmüş ancak NH3-N ve NH3-N/TN değerleri artmıştır. Mikrobiyal kompozisyon üzerine silolamanın olumlu etkileri olmuş maya ve küf değerleri düşmüştür (P<0.000).
Sonuç: Araştırma sonucunda %55 KM TRK’nın taze olarak tüketilmesi en ideali olmakla birlikte, aerobik stabilite açısından silolanmasının daha iyi olduğu söylenebilir.
Anahtar sözcükler: Aerobik stabilite, silolama süresi, sıcaklık sensörü, termal kamera, toplam rasyon karışımı

Kaynakça

  • Addah W, Baah J, Okine EK, McAllister T A. 2012. Use of thermal imaging and the in situ technique to assess the impact of an inoculant with feruloyl esterase activity on the aerobic stability and digestibility of barley silage. Canadian Journal of Animal Science, 92(3), 381-394.
  • Adesogan AT, Salawu MB. 2004. Effect of applying formic acid, heterolactic bacteria or homolactic and heterolactic bacteria on the fermentation of bi-crops of peas and wheat. Journal of Science of Food and Agriculture 84: 983-992.
  • Agma Okur A, Gozluklu K, Okur E, Okuyucu B, Koc F, Ozduven ML. 2022. Effects of apple vinegar addition on aerobic deterioration of fermented high moisture maize using ınfrared thermography as an indicator. Sensors, 22(3):771.
  • Anonymous 1986. The Analysis of Agricultural Material, Reference Book: 427. London, Pp. 428.
  • AOAC. 1990. In K. Helrich (Ed.), Official Methods of Analysis (15th ed.). Arlington, VA, USA: Association of Official Analytical Chemists, Inc.
  • Ashbell G, Weinberg ZG, Hen Y, Filya I. 2002. The effects of temperature on the aerobic stability of wheat and corn silages. J. Ind. Microbiol. Biotechnol. 28, 261–263.
  • Berk Z. 2018. Physical Properties of Food Materials. ln: Food Process Engineering and Technology. A volume in Food Science and Technology. Elsevier lnc. pp. 7-25.Available at: https://doi.org/10.1016/B978-0-12-373660-4.00001-6 (Cited 13 April 2020).
  • Coşkuntuna L, Erten K, Koç F. 2022. Toplam rasyon karışımının silolanmasının aerobik stabilite özellikleri üzerine etkisi. Türk Tarım ve Doğa Bilimleri Dergisi, 9(4), 933-942.
  • Driehuis F, Oude Elferink SJWH. 2000. The impact of the quality of silage on animal health and food safety: A review. Veterinary Quarterly, 22: 212-216.
  • Dubois M, Giles KA, Hamilton JK, Rebes PA, Smith F. 1956. Colorimetric method for determination of sugars and related substances. Analytical Chemistry, 28: 350-356.
  • Heikkila T, Saarisalo E, Taimisto AM, Jaakkola S. 2010. Effects of dry matter and additive on wilted bale silage quality and milk production. ln: Schnyder, H. et al. (Eds.) Grassland in a changing world: proceedings of the 23th General Meeting of the European Grassland Federation, Kiel, Germany August 29th - September 2nd 2010. Grassland Science in Europe 15: pp. 500-502.
  • Holmes BJ, Bolsen KK. 2009. What's new in silage management. ln: Broderick,
  • Jaakkola S, Saarisalo E, Heikkila T. 2010. Aerobic stability and fermentation quality of round bale silage treated with inoculants or propionic acid. Schnyder, H. et al. (Eds.) Grassland in a changing world: Proceedings of the 23th General Meeting of the European Grassland Federation, Kiel, Germany August 29th - September 2nd 2010. Grassland Science in Europe 15: 503-504.
  • Koç F, Özdüven ML, Demirci AŞ, Şamlı HE. 2018. Mısır silajlarında saha şartlarında aerobik stabilite süresince mikrobiyal kompozisyondaki değişikliklerin termal kamera görüntüleme tekniği ile değerlendirilmesi. Kahramanmaraş Sütçü İmam Üniversitesi Doğa Bilimleri Dergisi. 21 (2): 167-174
  • Koivunen E, Huuskonen A. 2018. Sailörehun hometoksiinit ja niiden vaikutukset nau- doilla. Luonnonvara- ja biotalouden tutkimus 19/2018. Luonnonvarakeskus, Helsinki, Finland. pp. 24. Available at: http://urn.fi/URN:lSBN:978-952-326-562-2 (Cited 24 November 2019). Kung L. Jr. 2005. Aerobic Stability of Silages. Proc. of the Conference on Silage for Dairy Farms. Harrisburg. Available at: https://www.academia.edu/6566050/Aerobic_Stabil-ity_of_Silages (Cited 20 October 2019).
  • Muck RE, Moser LE, Pitt RE. 2003. Postharvest factors affecting ensiling. ln: Bux- ton, D.R., Muck, R.E. & Harrison, J.H., (Eds.) Silage Science and Technology. Agronomy Publication No 42, American Society of Agronomy, Madison, Wisconsin USA. pp. 251-304.
  • Nishino N, Hattori H. 2007. Resistance to aerobic deterioration of total mixed ration silage inoculated with and without homofermentative or heterofermentative lactic acid bacteria. Journal of the Science of Food and Agriculture, 87(13), 2420-2426.
  • Ogunade M, Martinez-Tuppia C, Queiroz OCM, Jiang Y, Drouin P, Wu F, Vyas D, Adesogan AT. 2018. Silage review: Mycotoxins in silage: Occurrence, effects, prevention, and mitigation. Journal of Dairy Science 101: 4034-4059.
  • Pahlow G, Muck RE, Driehuis F, Elferink SJO, Spoelstra, SF. 2003. Microbiology of ensiling. Silage Science and Technology, 42, 31-93.
  • Pauly T, Wyss U. 2018. Methodology of ensiling trials and effects of silage additives. ln: Gerlach, K. & Südekum, K.-H. (Eds.). Proceedings of the XVlll lnternational Si- lage Conference, 24-26-July 2018, Bonn, Germany. p.196-209.
  • Pursiainen P, Tuori M. 2008. Effect of ensiling field bean, field pea and common vetch in different proportions with whole-crop wheat using formic acid or an inoculant on fermentation characteristics. Grass and Forage Science 63: 60-78.
  • Ranjit NK, Kung Jr L. 2000. The effect of Lactobacillus buchneri, Lactobacillus plantarum, or a chemical preservative on the fermentation and aerobic stability of corn silage. J. Dairy Sci. 83, 526–535.
  • Rinne M, Franco M, Kuoppala K, Seppala A, Jalava T. 2018. Response to total mixed ration stabilizers depends on feed quality. ln: Gerlach, K. & Südekum, K.-H. (Eds.). Proceedings of the XVlll lnternational Silage Conference, 24-26 July 2018, Bonn, Germany. p.538-539.
  • Rose D, Bianchini A, Martinez B, Flores R. 2012. Methods for reducing microbial contamination of wheat flour and effects on functionality. Cereal Foods World 57: 104-109.
  • Saarisalo E, Jalava T, Skytta E, Haikara A, Jaakkola S. 2006. Effects of dry matter and additive on wilted bale silage quality and milk production. Agricultural and Food Science 15: 185-199.
  • Seale DR, Pahlow G, Spoelstra SF, Lindgren S, Dellaglio F, Lowe JF, 1990. Methods for the microbiological analysis of silage. Grovfoder (Sweden).
  • Seppala A, Maki M, Orkola S, Rinne M. 2015. Aerobic stability of crimped barley ensiled with organic acids. ln: Uden, P. (Ed.) Proceedings of the 6th Nordic feed sci- ence conference, Uppsala, Sweden, 4-5 June, 2015. Swedish University of Agricul- tural Sciences. Department of Animal Nutrition and Management. Report 291: 71- 76.
  • Shah A.A, Qian C, Liu Z, Wu J, Sultana N, Mobashar M, Wanapat M, Zhong X 2021. Evaluation of biological and chemical additives on microbial community, fermentation characteristics, aerobic stability, and in vitro gas production of SuMu No. 2 elephant grass. J. Sci. Food Agric.101, 5429–5436.
  • Soycan Önenç S, Korkmaz Turgud F, Turan Uçman A. 2019. Kekik ve kimyon uçucu yağlarının yonca silajlarının fermantasyon kalitesi, aerobik stabilite ile yem değeri üzerine etkileri. Hayvansal Üretim, 60(2), 117-123.
  • Soysal Mİ. 1993. Biyometrinin Prensipleri (İstatistik I ve II Ders Notları), Yayın No: 95, Ders Kitabı No: 64, T. Ü. Tekirdağ Ziraat Fakültesi Tekirdağ.
  • Statistics for the Windows Operating System 1999. Stat Soft Inc., Tulsa, OK, USA.
  • Türkgeldi B, Koç F, Lackner M, Okuyucu B, Okur E, Palangi V, Esen S. 2023. Infrared thermography assessment of aerobic stability of a total mixed ration: An innovative approach to evaluating dairy cow feed. Animals, 13(13):2225.
  • Ünal Ö, Koç F, Okur AA, Okur E, Özdüven ML. 2018. Mısır ve buğday silajlarının termal kamera görüntüleme tekniği kullanılarak aerobik stabilitesinin değerlendirilmesi. Alınteri Journal of Agriculture Sciences, 33(1): 55-63.
  • Van Soest PV, Robertson JB, Lewis BA. 1991. Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597.
  • Wang F, Nishino N. 2008. Resistance to aerobic deterioration of total mixed ration silage: Effect of ration formulation, air infiltration and storage period on fermentation characteristics and aerobic stability. Journal of the Science of Food and Agriculture, 88(1), 133-140.
  • Weinberg ZG, Chen Y, Miron D, Raviv Y, Nahim E, Bloch A, Miron J. 2011. Preservation of total mixed rations for dairy cows in bales wrapped with polyethylene stretch film–A commercial scale experiment. Animal Feed Science and Technology, 164(1-2), 125-129.
  • Weiss K, Kroschewski B, Auerbach H. 2016. Effects of air exposure, temperature and additives on fermentation characteristics, yeast count, aerobic stability and volatile organic compounds in corn silage, Journal of Dairy Science 10: 8053-8069.
  • Wilkinson J M, Davies DR. 2013. The aerobic stability of silage: key findings and recent developments. Grass and Forage Science, 68, 1–19.
  • Xie Y, Wang L, Li W, Xu S, Bao J, Deng J, Wu Z, Yu Z. 2022. Fermentation quality, In vitro digestibility, and aerobic stability of total mixed ration silage in response to varying proportion alfalfa silage. Animals. 2022; 12(8):1039.
Toplam 39 adet kaynakça vardır.

Ayrıntılar

Birincil Dil İngilizce
Konular Hayvan Besleme
Bölüm Araştırma Makalesi
Yazarlar

Derya Türk 0000-0002-6477-0931

Kadir Erten 0000-0002-6307-1573

Fisun Koc 0000-0002-5978-9232

Erken Görünüm Tarihi 31 Aralık 2024
Yayımlanma Tarihi 31 Aralık 2024
Gönderilme Tarihi 13 Ağustos 2024
Kabul Tarihi 7 Kasım 2024
Yayımlandığı Sayı Yıl 2024 Cilt: 65 Sayı: 2

Kaynak Göster

APA Türk, D., Erten, K., & Koc, F. (2024). Effect of Ensiling Time on Microbial Composition and Aerobic Stability of Total Mixture Ration. Journal of Animal Production, 65(2), 119-129. https://doi.org/10.29185/hayuretim.1532368


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