Antioxidant, Antimicrobial and Cytotoxic Activities of Endemic Astragalus argaeus Boiss. from Turkey

Yıl 2019, Cilt: 47 Sayı: 1, 87 - 97, 01.02.2019

Sevil Albayrak , Onur Kaya

Öz

In this study, phenolic compound, the total phenolic and flavonoid contents, in vitro antioxidant, antiradical, hydrogen peroxide scavenging, metal chelating, total reducing power, iron ion reducing antioxidant potential (FRAP), copper (II) ion reducing antioxidant capacity (CUPRAC) activities of the extracts obtained from underground and aerial parts of Astragalus argaeus, which is endemic to Turkish flora were investigated. The ferulic acid has been identified as main compound in the both extracts. Both extracts showed weak antioxidant activities. The extracts showed weak antibacterial activity against only Pseudomonas aeruginosa among the 15 microorganisms by agar well diffusion assay. The cytotoxic effects of extracts on MCF-7 (human breast cancer cell lines) and fibroblast cells during 24 and 48 hours were determined by MTT method. The extracts showed weak cytotoxic activity on MCF-7 after 24h.

Anahtar Kelimeler

Astragalus argaeus, antimicrobial activity, antioxidant activity, cytotoxic

Kaynakça

• 1. M. Singh, N. Pandey, V. Agnihotri, K.K. Singh, A. Pandey, Antioxidant, antimicrobial activity and bioactive compounds of Bergenia ciliata Sternb.: A valuable medicinal herb of Sikkim Himalaya, J. Tradit. Complement Med., 7 (2017) 152- 157.
• 2. S. Ouerghemmi, H. Sebei, L. Siracusa, G. Ruberto, A. Saijac, F. Ciminoc, M. Cristani, Comparative study of phenolic composition and antioxidant activity of leaf extracts from three wild Rosa species grown in different Tunisia regions: Rosa canina L., Rosa moschata Herrm. and Rosa sempervirens L., Ind. Crop. Prod., 94 (2016) 167-177.
• 3. X.X. Zhang, Q.Q. Shi, D. Ji, L.X. Niu, Y.L. Zhang, Determination of the phenolic content, profile, and antioxidant activity of seeds from nine tree peony (Paeonia section Moutan DC.) species native to China, Food Res. Int,. 97 (2017) 141–148.
• 4. M. Bachir bey, L. Meziant, Y. Benchikh, H. Louaileche, Deployment of response surface methodology to optimize recovery of dark fresh fig (Ficus carica L., var. azenjar) total phenolic compounds and antioxidant activity, Food Chem., 162 (2014) 277–282.
• 5. L. Zhang, Z. Tu, X. Xie, Y. Lu, Z. Wang, H. Wang, Antihyperglycemic, antioxidant activities of two Acer palmatum cultivars, and identification of phenolics profile by UPLC-QTOF-MS/MS: New natural sources of functional constituents, Ind. Crop. Prod., 89 (2016) 522–532.
• 6. B.J. Djimtombaye, Ö.A. Çalışkan, D. Gülcemal, I.A. Khan, H. Anıl, E. Bedir, Unusual secondary metabolites from Astragalus halicacabus lam, Chem. Biodivers., 10 (2013) 1328-1334. S. Albayrak and O. Kaya / Hacettepe J. Biol. & C 96 hem., 2019, 47 (1), 87–97
• 7. J. Asgarpanah, S.M. Motamed, A. Farzaneh, B. Ghanizadeh, S. Tomraee, Antioxidant activity and total phenolic and flavonoid content of Astragalus squarrosus Bunge, Afr. J. Biotechnol,. 10 (2011) 19176-19180.
• 8. J. Chen, Y. Li, L.Q. Yang, Y.Z. Li, Z.B. Nan, K. Gao, Biological activities of flavonoids from pathogenic-infected Astragalus adsurgens, Food Chem., 131 (2012) 546-551.
• 9. F. Fathiazad, M.K. Khosropanah, A. Movafeghi, Cycloartanetype glycosides from the roots of Astragalus caspicus Bieb, Nat. Prod. Res., 24 (11) (2010) 1069–1078.
• 10. S. Jan, A. Abbaskhan., S.G. Musharraf, S.A. Sattar, Samreen, S.I. Resayes, Z.A. Al-Othman, A.M. Al-Majid, A. Rahman, M.I. Choudhary, three new cycloartane triterpenoids from Astragalus bicuspis, Planta Med., 77 (2011) 1829–1834.
• 11. S. Li-Man, W. Xiao-Ling, D. Wen-Long, D. Li-Sheng, P. Shu-Lin, Chemical Constituents from Astragalus ernestii. Chin. J. Nat. Med., 9 (1) (2011) 0038−0041.
• 12. W. Benchadi, H. Haba, C. Lavaud, D. Harakat, M. Benkhaled, Secondary Metabolites of Astragalus cruciatus Link. and Their chemotaxonomic significance, Rec. Nat. Prod., 7 (2013) 105-113.
• 13. D.H. Lim, D.B. Choi, O.Y. Choi, K.A. Cho, R. Kim, H.S. Choi, H. Cho, Effect of Astragalus sinicus L. seed extract on antioxidant activity, J. Ind. Eng. Chem., 17 (2011) 510-516.
• 14. J. Linnek, A.C. Mitaine-Offer, T. Miyamoto, C. Tanaka, T. Paululat, S. Avunduk, Ö.A. Calışkan, M.A. Lacaille-dubois, cycloartane glycosides from three species of Astragalus (Fabaceae), Helv. Chim. Acta., 94 (2011) 230-237.
• 15. M. Li, Y. Xu, W. Yang, J. Li, X. Xu, X. Zhang, F. Chen, D. Li, In vitro synergistic anti-oxidant activities of solvent-extracted fractions from Astragalus membranaceus and Glycyrrhiza uralensis, Food Sci. Technol., 44 (2011) 1745-1751.
• 16. R.M. Allam, D.A. Selim, A.I. Ghoneim, M.M. Radwan, S.M. Nofal, A.E. Khalifa, O.A. Sharaf, S.M. Toaima, A.M. Asaad, N.A. El-Sebakhy, Hepatoprotective effects of Astragalus kahiricus root extract against ethanol-induced liver apoptosis in rats, Chin. J. Nat. Med., 11 (4) (2013) 0354−0361.
• 17. V.L. Singleton, J.A.Jr. Rossi, Colorimetry of total phenolics with phosphomolybdic-phosphotungstic acid reagents, Am. J. Enol. Vitic., 16 (1965) 144-158.
• 18. F. Pourmorad, S.J. Hosseinimehr, N. Shahabimajd, Antioxidant activity, phenol and flavonoid contents of some selected Iranian medicinal plants, Afr. J. Biotechnol., 5 (2006) 1142-1145.
• 19. P. Prieto, M. Pineda, M. Aguilar, Spectrofotometric quantitation of antioxidant capacity through the formation of a Phosphomolybdenum Complex: specific application to the determination of vitamin E, Anal. Biochem., 269 (1999) 337-341.
• 20. S.K. Lee, Z.H. Mbwambo, H.S. Chung, L. Luyengi, E.J.C. Games, R.G. Metha, A.D. Kinghorn, J.M. Pezzuto, Evaluation of the antioxidant potential of natural products, Comb. Chem. High Throughput Screen., 1 (1998) 35-46.
• 21. L. Cao, J.Y. Si, Y. Liu, H. Sun, W. Jin, Z. Li, X.H. Zhao, R.L. Pan, Essential oil composition, antimicrobial and antioxidant properties of Moslachinensis Maxim, Food Chem., 115 (2009) 801-805.
• 22. R. Apak, K. Güçlü, M. Özyurek, S.E. Karademir, E. Ercağ, The cupric ion reducing antioxidant capacity and polyphenolic content of some herbal teas, Int. J. Food Sci. Nutr., 57 (2006) 292-304.
• 23. C.I.G. Tuberoso, P. Montoro, S. Piacente, G. Corona, M. Deiana, M.A. Dessi, C. Pizza, P. Cabras, Flavonoid characterization and antioxidant activity of hydroalcoholic extracts from Achillea ligustica All, J. Pharm. Biomed. Anal., 50 (2009) 440-448.
• 24. A. Peksel, İ. Arisan-Atac, R. Yanardag, Evaluation of antioxidant and antiacetylcholinesterase activities of the extracts of Pistacia atlantica desf. Leaves, J. Food Biochem., 34 (2010) 451-476.
• 25. K.R. Aadil, A. Barapatre, S. Sahu, H. Jha, B.N. Tiwary, Free radical scavenging activity and reducing power of Acacia nilotica wood lignin, Int. J. Biol. Macromol., 67 (2014) 220- 227.
• 26. S. Albayrak, A. Aksoy, O. Sagdic, E. Hamzaoglu, Compositions, antioxidant and antimicrobial activities of Helichrysum (Asteraceae) species collected from Turkey, Food Chem., 119 (2010) 114-122.
• 27. A.S. Zarena, B. Manohar, K. Udaya-Sankar, Optimization of supercritical carbon dioxide extraction of xanthones from mangosteen pericarp by response surface methodology, Food Bioproc. Tech,. 5 (2012) 1181–1188.
• 28. İ. Gülçin, V. Mshvildadze, A. Gepdiremen, R. Elias, Antioxidant activity of a triterpenoid glycoside isolated from the berries of Hedera colchica: 3-o-(β-d-glucopyranosyl)-hederagenin, Phytother. Res., 20 (2006) 130–134.
• 29. E. Bedir, I. Çalıs, R. Aquino, S. Piacente, C. Pizza, Trojanoside H: a cycloartane-type glycoside from the aerial parts of Astragalus trojanus, Phytochemistry., 51 (1999) 1017-1020.
• 30. I. Çalış, A.A. Dönmez, A. Perrone, C. Pizza, S. Piacente, Cycloartane glycosides from Astragalus campylosema Boiss. ssp. Campylosema, Phytochemistry., 69 (2008) 2634–2638.
• 31. N. Denizli, I. Horo, D. Gülcemal, M. Masullo, M. Festa, A. Capasso, Ö. Koz, S. Piacente, Ö.A. Çalışkan, Cycloartane glycosides from Astragalus plumosus var. krugianus and evaluation of their antioxidant potential, Fitoterapia., 92 (2014) 211–218.
• 32. D. Gülcemal, O. Alankuş-Calışkan, A. Perrone, F. Ozgokçe, S. Piacente, E. Bedir, Cycloartane glycosides from Astragalus aureus, Phytochemistry., 72 (2011) 761–768.
• 33. D. Gülcemal, M. Masullo, E. Bedir, M. Festa, T. Karayıldırım, Ö. Alankuş-Çalışkan, S. Piacente, Triterpene glycosides from Astragalus angustifolius, Planta Med,. 78 (2012) 720–729.
• 34. I. Horo, E. Bedir, A. Perrone, F. Özgökçe, S. Piacente, Ö.A. Çalışkan, Triterpene glycosides from Astragalus icmadophilus, Phytochemistry., 71 (2010) 956–963.
• 35. I. Horo, E. Bedir, M. Masullo, S. Piacente, F. Özgökçe, Ö.A. Çalışkan, Saponins from Astragalus hareftae (NAB.) SIRJ, Phytochemistry. , 84 (2012) 147–153.
• 36. F. Karabey, I.A. Khan, E. Bedir, Cycloartane-type glycosides from Astragalus schottianus, Phytochemistry., 5 (2012) 320- 324.
• 37. E. Polat, Ö. Alankuş-Çalıskan, A. Perrone, S. Piacente, E. Bedir, Cycloartanetype glycosides from Astragalus amblolepis, Phytochemistry., 70 (2009) 628–634.
• 38. E. Polat, E. Bedir, A. Perrone, S. Piacente, Ö. Alankuş-Çalışkan, Triterpenoid saponins from Astragalus wiedemannianus, Phytochemistry., 71 (2010) 658–662.
• 39. T. Savran, D. Gülcemal, M. Masullo, T. Karayıldırım, E. Polat, S. Piacente, Ö.A. Çalışkan, Cycloartane Glycosides from Astragalus erinaceus, Rec. Nat. Prod., 6:3 (2012) 230-236.
• 40. F.N. Yalçın, S. Piacente, A. Perrone, A. Capasso, H. Duman, I. Çalıs, Cycloartane glycosides from Astragalus stereocalyx Bornm, Phytochemistry., 73 (2012) 119–126.
• 41. L. Pistelli, A. Bertoli, E. Leporia, I. Morellia, L. Panizzi, Antimicrobial and antifungal activity of crude extracts and isolated saponins from Astragalus errucosus, Fitoterapia. 73 (2002) 336–339.
• 42. F. Abbas, R. Zayed, Bioactive saponins from Astragalus suberi L. growing in Yemen, Z Naturforsch. 60 (11–12) (2005) 813–820. S. Albayrak and O. Kaya / Hacettepe J. Biol. & Chem., 2019, 47 (1), 87–97 97
• 43. S. Balachandar, M. Jagadeeswari, R. Dhanabalan, M. Meenachi, Antimicrobial activity of Astragalus membranaceus against diarrheal bacterial pathogens, Int. J. Pharm. 2(2) (2012) 416-418.
• 44. A. Adıgüzel, M. Sökmen, H. Özkan, G. Ağar, M. Güllüce, F. Şahin, In vitro antimicrobial and antioxidant activities of methanol and hexane extract of Astragalus species growing in the eastern Anatolia region of Turkey, Turk. J. Biol., 33 (2009) 65-71.
• 45. D. Akyil, S.F. Erdogmus, Y. Eren, A. Ozkara, E. Korcan. Potential antimicrobial and antimutagenic activities of Astragalus flavescens, Fresen. Environ. Bull., 22 (7) (2013)1868-1873.
• 46. A.U. Türker, A.B. Yıldırım, Evaluation of antibacterial and antitumor activities of some Turkish endemic plants, Trop. J. Pharm. Res., 12 (2013) 1003-1010.
• 47. H. Teyeb, N. Zanina, M. Neffati, W. Douki, M.F. Najjar, Cytotoxic and antibacterial activities of leaf extracts of Astragalus gombiformis Pomel (Fabaceae) growing wild in Tunisia, Turk. J. Biol., 36 (2012) 53-58.