Development and Validation of a Quantification Method for L-DOPA in Plants and Pharmaceutical Materials

Yıl 2021, Cilt: 49 Sayı: 1, 1 - 10, 01.01.2021

İbrahim Bulduk , Nurdoğan Topal

https://doi.org/10.15671/hjbc.773988

Cited By: 3

Öz

L-DOPA is an antiparkinsonian drug and is used per oral application. A reliable liquid chromatographic method for determination of L-DOPA in leaves, flowers and seeds of Vicia faba L. grown in Turkish habitats and pharmaceutical dosage forms has been developed. The analysis has been carried out using Ace C18 (5 µm, 4.6 x 250 mm,) column, and the separation was carried out using a mobile phase consisting of 50 mM potassium dihydrogen phosphate (pH 2.3) at a flow rate of 1.2 mL min-1 with UV detection at 280 nm. The method has been validated according to the acceptance criteria of the ICH guidelines. The method demonstrated good linearity (R2 > 0.999) over the assayed concentration range and showed good intra-day and inter-day precision. The detection limit (LOD) and the quantification limit (LOQ) values were determined as 1.70 and 5.13 μg mL-1 for L-DOPA. This method has been successfully used for the determination of L-DOPA in pharmaceutical formulations and plant materials.

Anahtar Kelimeler

L-DOPA, HPLC-UV, METHOD, DEVELOPMENT, PLANT MATERIAL

Kaynakça

• S. Fahn, D. Oakes, I. Shoulson, K. Kieburtz, A. Rudolph, A Lang, C.W. Olanow, C. Tanner, K. Marek, L-DOPA and the progression of Parkinson's disease, New Engl. J. Med., 351(2004) 2498–2508.
• C.E. Clarke, M. Guttman. Dopamine Agonist Monotherapy in Parkinson's Disease. Lancet, 360 (2002) 1767–1769.
• M. Rezak, Current pharmacotherapeutic treatment options in Parkinson's disease, Dis. a Month, 53 (2007) 214–222.
• D. Goncalves, G. Alves, P. Soares-da-Silva, A. Falcão, Bioanalytical chromatographic methods for the determination of catechol-O-methyltransferase inhibitors in rodents and human samples: a review, Anal. Chim. Acta, 710 (2012) 17–32.
• F.J. David, M.R. Rafferty, J.A. Robichaud, J. Prodoehl, W.M. Kohrt, D.E. Vaillan-court, D.M. Corcos, Progressive resistance exercise and Parkinson's disease: a review of potential mechanisms, Parkinson’s Dis, (2011) 124527.
• J.M. Cedarbaum, R. Williamson, H. Kutt, Simultaneous determination of L-DOPA, its metabolites and carbidopa in clinical samples, J. Chromatogr. B: Biomed. Sci. Appl., 415 (1987) 393–399.
• Y. Michotte, M. Moors, D. Deleu, P. Herregodts, G. Ebinger, Simultaneous Determination of Levodopa, Carbidopa, 3-O-methyldopa and dopamine in plasma using high-performance liquid chromatography with electrochemical detection, J. Pharm. Biomed. Anal., 5 (1987) 659–664.
• D.C. Titus, T.F. August, K.C. Yeh, R. Eisenhandler, W.F. Bayne, D.G. Musson, Simultaneous high-performance liquid chromatographic analysis of carbidopa, L-DOPA and 3-O-methyldopa in plasma and carbidopa, L-DOPA and dopamine in urine using electrochemical detection, J. Chromatogr. B: Biomed. Sci. Appl., 534 (1990) 87–100.
• K.A. Sagar, M.R. Smyth, Simultaneous determination of L-DOPA, carbidopa and their metabolites in human plasma and urine samples using LC-EC, J. Pharm. Biomed. Anal., 22 (2000) 613–624.
• V. Rizzo, M. Memmi, R. Moratti, G. Melzi d’Eril, E. Perucca, Concentrations of L-dopa in plasma and plasma ultrafiltrates. J. Pharm. Biomed. Anal., 14 (1996) 1043–1046.
• T. Wikber, Simultaneous determination of L-DOPA, its main metabolites and carbidopa in plasma by liquid chromatography, J. Pharm. Biomed. Anal., 9 (1991) 167–176.
• M. Karimi, J.L. Carl, S. Loftin. J.S. Perlmutter, Modified high-performance liquid chromatography with electrochemical detection method for plasma measurement of L-DOPA, 3-O-methyldopa, dopamine, carbidopa and 3,4-dihydroxyphenyl acetic acid. Journal of chromatography. B, Analytical Technologies in the Biomedical and Life Sciences, 836 (2006) 120-123.
• F. Bugamelli, C. Marcheselli, E. Barba, M.A. Raggi, Determination of L‐dopa, carbidopa, 3‐O‐methyldopa and entacapone in human plasma by HPLC‐ED. Journal of Pharmaceutical and Biomedical Analysis, 54 (2011) 562–567.
• H.F. Martins, D.P. Pinto, d.A.V. Nascimento, M.A.S. Marques, F.C. Amendoeira, Development of an HPLC/MS/MS methodology for determining 3-Omethyldopa in human plasma and its application in a bioequivalence study, Quím. Nova, 36 (2013) 171–176.
• K. Igarashi, K. Hotta, F. Kasuya, K. Abe, S. Sakoda, Determination of cabergoline and L-dopa in human plasma using liquid chromatography-tandem mass spectrometry, J. Chromatogr. B, 792 (2003) 55–61.
• R.R. Gonzalez, R.F. Fernandez, J.L. Vidal, A.G. Frenich, M.L. Perez, Development and validation of an ultra-high performance liquid chromatography-tandem mass-spectrometry (UHPLC-MS/MS) method for the simultaneous determination of neurotransmitters in rat brain samples, Neurosci. Methods, 198 (2011) 187–194.
• P.O. Cinto, A.L.R.S. Souza, A.C. Lima, M.V. Chaud, M.P.D. Gremião, LC Evaluation of Intestinal Transport of Praziquantel. Chromatographia, 69 (2009) 213–217.
• R. Hadjikinova, N. Petkova, D. Hadjikinov, P. Denev, D. Hrusavov, Development and Validation of HPLC-RID method for Determination of Sugars and Polyols. J. Pharm. Sci. & Res., 9 (2017)1263-1269.