Investigation of Immunological Infertility in Repeat Breeder Cows

Yıl 2018, Cilt: 7 Sayı: 1, 89 - 92, 04.07.2018

B. Kemal Gümüşay , İshak Gökçek , İlker Yavaş

https://doi.org/10.31196/huvfd.470816

Öz

In
terms of the future of livestock businesses, obtaining calf from cows is
crucial. There are many factors that prevent fertility. These factors include
hereditary and functional problems (an- oestrus, sub-oestrus, cysts),
infectious diseases, and miscarriage. It is noteworthy that some cows do not
become pregnant despite the numerous inseminations even in the absence of an
appearent reason. In this
study it was aimed to investigate the incidence of immunological infertility by
determining immunglobulin levels in repeat breeder cows. In the study 50 cows
which calved at least once and were not pregnant despite at least 3 subsequent
inseminations although they did not have any disorders preventing fertility as
assessed by rectal and ultrasonographic examinations, were used. Antisperm
antibodies (ASA) were detected by ELISA test in blood serum samples obtained
after insemination. As a result, ASA were detected in 14.33% of the
cows that were at least 3 times inseminated but not conceived at any time in
normal field conditions.

Anahtar Kelimeler

Infertility, Immunological infertility, Secretory immuneglobulin A, Artificial insemination

Döl Tutmayan İneklerde İmmunolojik İnfertilitenin Araştırılması

Öz

Hayvancılık
işletmelerinin geleceği açısından ineklerden yavru elde edilmesi çok önemli yer
teşkil eder. İneklerde infertilite
nedenleri çok çeşitlidir. Bunlar arasında herediter, işlevsel (anöstrus, suböstrus,
kistler), enfeksiyöz hastalıklar, bakım ve besleme bozuklukları sayılabilir. Günümüzde
yukarıda sayılan bu nedenlerin bulunmadığı durumlarda bile yapılan çok sayıda
tohumlamaya rağmen bazı ineklerde gebe kalamama olgularının görülmesi dikkat
çekicidir. Bu çalışmada infertil
ineklerde immunglobulin seviyelerine bakılarak immünolojik infertilitenin
tespiti ve immünolojik infertilitenin görülme sıklığının araştırılması
amaçlanmıştır. Çalışmada,
özel işletmelerde bulunan ve en az 3 kere tohumlandığı halde gebe kalmayan, rektal
ve ultrason ile yapılan muayenede fertiliteye engel herhangi bir bozukluğu bulunmayan,
daha önce en az bir kere sorunsuz doğum yapmış inekler kullanılmıştır.
Tohumlama sonrası elde edilen kan serumu örneklerinde ELISA testi ile antisperm
antikorları tespit edilmiştir. Sonuç
olarak, normal saha koşullarında test edilen herhangi bir rahatsızlığı
bulunmayan, en az 3 kere tohumlanmış ama gebe kalmamış ineklerin kan
serumlarında ELISA testi ile % 14,33 oranında antisperm antikorları (ASA) tespit
edilmiştir. 

Anahtar Kelimeler

İnfertilite, İmmunolojik infertilite, Salgısal immunglobülin A, Suni tohumlama

Kaynakça

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