Türk popülasyonunda bronkopulmoner displazi insidansı ile ilişkisinin araştırılması için rs3771150 polimorfizminin genotiplenmesi ve analizi

Yıl 2018, Cilt: 4 Sayı: 1, 16 - 21, 18.04.2018

Ayberk Akat Seda Yılmaz Semerci , Osman Mutluhan Uğurel Ayşegül Erdemir Merih Çetinkaya Dilek Turgut-balık

Öz

Amaç: Bu çalışmada Türkiye’de erken doğan bebeklerde görülen BPD insidansı ile IL-18RAP üzerinde bulunan rs3771150 polimorfizmi arasındaki ilişki araştırılmıştır. 

Materyal ve Metod: Bu polimorfizmin alel ve genotip frekansları multipleks reaksiyonlar ve MALDI-TOF kütle spektrometresi yöntemi ile elde edilen genotipleme sonuçları kullanılarak analiz edilmiştir. Buna ek olarak hastalardan elde edilen klinik ve demografik veriler de BPD insidansı ile ilişkileri açısından değerlendirilmiştir. 

Bulgular: Türk popülasyonu için rs3771150 polimorfizminin yabanıl genotipi GG olarak belirlenmiştir (n=49 kontrol, n=46 BPD hastası). Diğer genotipler (AG ve AA) analiz edildiğinde BPD insidansı ile anlamlı bir ilişki kurulamamıştır (p>0.05).

Sonuç: Erken doğan 192 Türk bebekte rs3771150 polimorfizmi ile BPD insidansı arasında anlamlı bir ilişki bulunamamıştır.

Anahtar Kelimeler

Bronchopulmonary dysplasia, multiplex polymerase chain reaction, single nucleotide polymorphism, SNP genotyping

Genotyping and analysis of rs3771150 polymorphism to investigate an association with bronchopulmonary dysplasia incidence in Turkish population

Öz

Objectives: rs3771150 polymorphism on IL-18RAP was chosen to be investigated in terms of determining a relationship with BPD incidence seen in preterm infants born in Turkey. 

Materials and Method: Allele and genotype frequencies of this polymorphism was analyzed from genotyping results obtained by multiplex reactions and MALDI-TOF mass spectrometry method. In addition, clinical and demographic data obtained from the patients were also inquired in this study to determine any relationship with BPD incidence. 

Results: GG genotype has been determined as the wild type genotype of rs3771150 polymorphism for the Turkish population (n=49 in control group, n=46 in BPD group). When other genotypes (AG and AA) were analyzed no significant relationship was found with BPD incidence (p>0.05).

Conclusion: No significant relationship could be determined between rs3771150 polymorphism and BPD incidence in 192 preterm Turkish infants.

Kaynakça

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