MOLECULAR CHARACTERIZATION OF DIFFERENT CURRANT TYPES

Yıl 2017, Cilt: 1 Sayı: 1, 21 - 26, 21.12.2017

Ayşen Melda Çolak , Fatma Alan

Öz

When berries are of the case, Strawberry, Raspberry, Blackberry, Currants, Gooseberry Blueberries, Elderberry, Wild spindle, Rosehip, Berberis and Sloe generally come to mind and they are fruit types that are consumed deliciously in the world. Their conservation in fresh, following the harvest, their long storage in cold stores and their marketing bear great importance with respect to other fruits. In DNA isolation of berries, more and qualified DNA is obtained by using fresh leaf tissue than old leaf tissue. In this paper, DNAs isolated with the method of CTAB DNA isolation from the leaf of five different currant types (Jostaberry, Redlake, Goliath, Rovada and Boskopgiant) bought Eymen Plantation in Bursa-Kestel province of Turkey are companed with each other in terms of their quantity and quality. Purity and quantity and DNAs which are obtained from young leaves are determined both by gel electrophoresis measurements and UV spectroscopic one. Whether there happens to be genetic variations between the types according to DNA resource is determined by applying ISSR primer and PCR to the DNAs obtained in the study.

Anahtar Kelimeler

Currant, Genetic variations, Molecular marker

Kaynakça

• Brennan, R. M., 2008. Currants and Gooseberries, Temperate Fruit Crop Breeding, J.F. Hancock (ed.), Chapter 6, 177-196, Scotland, UK. Eyduran, S. P., Ağaoğlu, Y. S., 2007., Some Pomological and Vegetative Characteristics of the Currants Cultivated in Ankara (Ayas) Conditions Ankara University Faculty of Agriculture, Journal of Agricultural Sciences 13 (3) 293-298. Hayden, R. A., Dana, M. N., Lerner, B. R., 1987. Currants and Gooseberries. HOPurdue University, Cooperative Extension Service, no.17, p.2. Ġslam, A., 2010. Grape Fruit Growing Undergraduate Course Note (Unpublished), Ordu University Faculty of Agriculture Department of Horticulture, .42 s. Mitchell, C., Brennan, R. M., Cross, J. V., Johnson, S. N., 2011. Arthropod Pests Of Currant and Gooseberry Crops in The U.K. : Their Biology, Management and Future Prospects. Agricultural and Forest Entomology, 13, 221–237. Okatan, V., Polat, M., AĢkın, M. A., & Çolak, A. M. (2015). Frenküzümü (Ribes spp.), Jostaberry (Ribes x nidigrolaria Bauer) ve BektaĢi Üzümünün (Ribes grossularia L.) Fiziksel Özelliklerinin Belirlenmesi. Süleyman Demirel Üniversitesi Ziraat Fakültesi Dergisi 10 (1): 83-89. Okatan, V., GÜNDOĞDU, M., GÜÇLÜ, S. F., ÇELĠKAY, A., ÖZAYDIN, A. M. Ç., KORKMAZ, N., ... & AġKIN, M. A (2017). Phenolic Profiles of Currant (Ribes spp.) Cultivars. YYÜ TAR BĠL DERG (YYU J AGR SCI), 27(2): 192-196 Okatan, V., & AġKIN, M. A. (2017). THE EFFECTS OF DIFFERENT GROWING SYSTEMS ON THE YIELD AND QUALITY OF CURRANT CULTIVATION. Scientific Papers. Series B, Horticulture. Vol. LXI, 21-26. Rohlf., 2004. Numerical Taxonomy Multivariate Analysis System, NTSYS-pc version 2.11, Exeter Software, Setauket, N.Y. USA. Uzun, A., 2009. Characterization of Genetic Diversity in Citrus with SRAP Markers, Doctoral Thesis, Cukurova University, Institute of Science and Technology, Adana.