Effects of Some Extraction Solvents on the Antioxidant Properties of Strawberry Fruit

Yıl 2018, Cilt: 4 Sayı: 2, 102 - 109, 30.12.2018

Nagihan M. Karaaslan , Merve Goksin Karaaslan , Burhan Ates

https://doi.org/10.29132/ijpas.354885

Cited By: 6

Öz

In this
paper, antioxidant activity of strawberries extracted with different solvents was
investigated since they are consumed by people due to their anticancer,
anti-atherosclerotic, anti-inflammatory and anti-neurodegenerative properties.
For this purpose, different acidified extraction solvents such as water (ES1), acetone (ES2), acetonitrile (ES3),
methanol (ES4) and ethanol (ES5) were used in extraction process of
strawberries. The effects of different extraction solvents on the antioxidant
activity were evaluated by measuring the reducing power, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid
(ABTS) radical scavenging activity, 1,1-diphenyl-2-picrylhydrazyl
(DPPH) radical scavenging activity, total flavonoid content (TFC) and total phenolic content (TPC). Experimental
results indicated that the obtained data varied with different extraction
solvents. All antioxidant activity assay results showed that the highest results
were obtained with acetone extracts.

Anahtar Kelimeler

antioxidant activity, extraction solvent, strawberry fruit

Çilek Meyvesinin Antioksidan Özellikleri Üzerine Bazı Ekstraksiyon Solventlerinin Etkileri

Öz

Bu makalede,
antikanser, anti-aterosklerotik, anti-enflamatuar ve anti-nörodejeneratif
özelliklerinden dolayı insanlar tarafından tüketilen çileklerin farklı
ekstraksiyon çözücülerindeki antioksidan aktiviteleri araştırılmıştır. Bu amaç
için, çileklerin ekstraksiyon işleminde su (ES1),
aseton (ES2), asetonitril (ES3), metanol (ES4) ve etanol (ES5) gibi
farklı asitlendirilmiş ekstraksiyon çözücüleri kullanılmıştır. Antioksidan aktiviteye
farklı ekstraksiyon çözücülerinin etkileri indirme gücü, 2,2'-azino-bis
(3-etilbenzotiazolin-6-sülfonik asit (ABTS) radikal süpürme aktivite,
1,1-difenil-2-pikrilhidrazil (DPPH) radikal süpürme aktivite, toplam flavonoid
içerik (TFC) ve toplam fenolik içerik (TPC) analizleri kullanılarak
değerlendirilmiştir. Deneysel sonuçlar elde edilen dataların farklı ekstraksiyon
çözücüleri ile değiştiğini göstermiştir. Tüm antioksidan aktivite analiz
sonuçları, en yüksek sonuçların asetonlu ekstraklardan elde edildiğini
göstermiştir.

Anahtar Kelimeler

Antioksidan aktivite, ekstraksiyon çözücüsü, çilek

Kaynakça

• Aaby, K., Skrede, G., Wrolstad, R.E., 2005. Phenolic composition and antioxidant activities in flesh and achenes of strawberries (Fragaria ananassa). Journal of Agricultural and Food Chemistry, 53(10):4032-4040.
• Bazzano, L.A., 2005. Dietary intake of fruit and vegetables and risk of diabetes mellitus and cardiovascular diseases, In: Background Paper of the Joint FAO/WHO Workshop on Fruit and Vegetables for Health. World Health Organization, Kobe, Japan, 1-65.
• Boulekbache-Makhlouf, L., Medouni, L., Medouni-Adrar, S., Arkoub, L., Madani K., 2013. Effect of solvents extraction on phenolic content and antioxidant activity of the byproduct of eggplant, Industrial Crops and Products, 49:668-674.
• Brand-Williams, W., Cuvelier, M.E., Berset, C., 1995. Use of a free-radical method to evaluate antioxidant activity. Food Science and Technology-Lebensmittel-Wissenschaft &Technologie, 28(1):25-30.
• Cerezo, A.B., Cuevas, E., Winterhalter, P., Garcia-Parrilla, M.C., Troncoso, A.M., 2010. Isolation, identification, and antioxidant activity of anthocyanin compounds in Camarosa strawberry. Food Chemistry, 123(3):574-582.
• Dorman H.J.D., Hiltunen, R., 2004. Fe (III) reductive and free radical-scavenging properties of summer savory (Satureja hortensis L.) extract and subfractions. Food Chemistry, 88(2):193-199.
• Dyduch-Sieminska, M., Najda, A., Dyduch, J., Gantner, M., Klimek, K., 2015. The content of secondary metabolites and antioxidant activity of wild strawberry fruit (Fragaria vesca L.), Journal of Analytical Methods in Chemistry Article number: 831238.
• Hayouni, E.A., Abedrabba, M., Bouix, M., Hamdi, M., 2007. The effects of solvents and extraction on the phenolic contents and biological activities in vitro of Tunisian Quercus coccifera L. and Juniperus phoenicea L. fruit extracts. Food Chemistry, 105(3):1126-1134.
• Kim, Y.-J., Shin, Y., 2015. Antioxidant profile, antioxidant activity, and physicochemical characteristics of strawberries from different cultivars and harvest locations, Journal of the Korean Society for Applied Biological Chemistry, 58(4):587-595.
• Marioda, A.A., Ibrahim, R.M., Ismail, M., Ismail N., 2012. Antioxidant activity of phenolic extracts from kenaf (Hibiscus Cannabinus) seedcake, Grasas y Aceites, 63(2):167-174.
• Mitić, M.N., Kostić, D.A., Pavlović, A.N., Imitrijevic, D.S., Veljkovic, J.N., 2014. Effects of solvent extraction system on concentration and antioxidant activity of strawberry phenolics, Agro Food Industry Hi-Tech, 25(5):24-28.
• Oyaizu, M., 1988. Antioxidative activities of browning products of glucosamine actionated by organic solvent and thin layer chromatography. Nippon Shokuhin Kogyo Gakkaishi 35:771-775.
• Peterson, J., Dwyer, J., 1998. Flavonoids: Dietary occurrence and biochemical activity. Nutrition Research, 18(12):1995-2018.
• Prior, R.L., Wu, X., Schaich, K., 2005. Standardized methods for the determination of antioxidant capacity and phenolics in foods and dietary supplements. Journal of Agricultural and Food Chemistry, 53(10):4290-4302.
• Re, R., Pellegrini, N., Proteggente, A., Pannala, A., Yang, M., Rice-Evans, C., 1999. Antioxidant activity applying an improved ABTS radical cation decolorization assay. Free Radical Biology and Medicine, 26(9-10):1231-1237.
• Reis, B., Martins, M., Barreto, B., Milhazes, N., Garrido, E.M., Silva, P., Borges, F., 2010. Structure-property-activity relationship of phenolic acids and derivatives. Protocatechuic acid alkyl esters. Journal of Agricultural and Food Chemistry, 58(11):6986-6993.
• Saini, R., Garg, V., Dangwal, K., 2013. Effect of extraction solvents on polyphenolic composition and antioxidant, antiproliferative activities of Himalyan bayberry (Myrica esculenta). Food Science and Biotechnology, 22(4):887-894.
• Santas, J., Carbó, R., Gordon, M.H., Almajano, M.P., 2008. Comparison of the antioxidant activity of two Spanish onion varieties. Food Chemistry, 107(3):1210-1216.
• Seal, T., Halder, N., Chaudhuri, K., Sinha S.N., 2015. Evaluation of antioxidant activities of algae and effect of solvent extraction system, International Journal of Pharmaceutical Sciences and Research, 6(3):1273-1278.
• Seeram, N.P., Lee, R., Scheuller, H.S., Heber, D., 2006. Identification of phenolic compounds in strawberries by liquid chromatography electrospray ionization mass spectroscopy. Food Chemistry, 97(1):1-11.
• Singleton, V.L., Rossi, J.A., 1965, Colorimetry of total phenolics with phosphomolybdic–phosphotungstic acid reagents. American Journal of Enology and Viticulture, 16:144-158.
• Sun, T., Ho C.T., 2005. Antioxidant activities of buckwheat extracts, Food Chemistry, 90(4):743-749.
• Ubeda, C., Callejón, R.M., Hidalgo, C., Torija, M.J., Troncoso, A.M., Morales, M.L., 2013. Employment of different processes for the production of strawberry vinegars: Effects on antioxidant activity, total phenols and monomeric anthocyanins. LWT - Food Science and Technology, 52(2):139-145.
• Wang, H., Helliwell, K., 2001. Determination of flavonols in green and black tea leaves and green tea infusions by high-performance liquid chromatography. Food Research International, 34(2-3):223-227.
• Wang, S.Y., Lin, H.S., 2000. Antioxidant activity in fruits and leaves of blackberry, raspberry and strawberry varies with cultivar and developmental stage. Journal of Agricultural and Food Chemistry, 48(2):140-146.
• WCRF/AICR, 2007. Food, Nutrition, Physical Activity and the Prevention of Cancer: A global perspective. world cancer research fund/American institute for cancer research, Washington, DC, USA.
• Wolfe, K.L., Kang, X., He, X., Dong, M., Zhang, Q., Liu, R.H., 2008. Cellular antioxidant activity of common fruits. Journal of Agricultural and Food Chemistry, 56(18):8418-8426.
• Zhishen, J., Mengcheng, T., Jianming, W., 1999. The determination of flavonoid contents in mulberry and their scavenging effects on superoxide radicals. Food Chemistry, 64(4):555-559.