Bacillus subtilis Bakterisi ile Metribuzin Herbisitinin Biyoıslahının Yapay Tarla Düzeneğinde Araştırılması

Yıl 2019, Cilt: 5 Sayı: 1, 46 - 52, 01.07.2019

Gökhan Önder Ergüven

https://doi.org/10.29132/ijpas.529882

Cited By: 2

Öz

Türkiye’de domates yetiştiriciliğinde seçkin bir
herbisit olan Metribuzinin (C8H14N4OS) biyoıslahı
Bacillus subtilis bakterisiyle
çalkalanmalı kültür koşullarında ve yapay tarla düzeneğine araştırılmıştır.
Bakteri Türkiye Elazığ ilinden bir tarım arazisinden toplanan topraktan izole
edilmiştir. Bölge daha önce metribuzine maruz kalmayan bir bölgedir. 1750 mg L-1
konsantrasyonda (çiftçler için tavsiye edilen konsantrasyon)  100’er mL’lik beş adet aparat hazırlanmıştır
ve metribuzin her bir erlenmayer şişesine ilave edilmiştir. Her bir
zenginleştirilen solüsyon ×
10⁷ Koloni oluşturan birey (KOB) içermektedir. Bu şişeler 160 rpm de
28°C’de steril koşullarda 17 gün boyunca
çalkalanmıştır. 24 saatlik dilimlerde, her bir örnek toplanmıştır ve DR/890
kolorimetre ile kimyasal oksijen ihtiyacı (KOİ) kapalı refluks yöntemi ışığında
belirlenirken Biyokimyasal Oksijen İhtiyacı (BOİ₅) standart method
5210D ye göre oksitoplarla belirlenmiştir. Çalışma sonuçlarına göre en iyi
giderim performansı 100 mL Bacillus
subtilis türünde KOİ ve BOİ₅ bazında 17 günde %98 oranında
görülmüştür. Yapay tarla çalışmasının sonuçlarına göre KOİ ve BOİ₅ parametrelerinde
2. haftanın sonunda sırasıyla %99.4 ve %96 olarak görülmüştür. Bu deneyler
göstermiştir ki Bacillus subtilis alıcı
ortamlardaki metribuzin gieriminde seçkin bir türdür.

Anahtar Kelimeler

Bakteri, biyoıslah, herbisit, metribuzin

Investigation of the Bioremediation of Herbicide Metribuzin with Bacillus subtilis Bacteria at Artifical Agricultural Field

Öz

The bioremoval of
metribuzin (C₈H₁₄N₄OS) which is one of the
selective herbicide in tomato farming in Turkey was investigated with Bacillus subtilis bacteria under
agitated culture conditions and artifical agricultural field. The bacterium was
isolated in collected soil samples from one of the agricultural area in Elazig
province of Turkey. The area is unexposed to metribuzin before. Five
apparatuses were set up and 100 mL of metribuzin in 1750 ppm concentration
(advised concentration of tomato farmers) was added to each Erlenmayer flasks.
5, 10, 20, 50 and 100 mL of enriched Bacillus
subtilis solutions (in sabouraud dextrose broth) added to these flasks.
Each mL of the enriched solutions contains ×10⁷ Colony forming unit (CFU).
These flasks were shaken at 160 rpm at 28 ⁰C in sterile conditions
for 17 days. In 24 hours intervals, each sample was collected to flasks and
chemical oxygen demand (COD) was determined with DR/890 colorimeter by the line
of closed reflux method while Biochemical oxygen demand (BOD₅)experiments
were performed via standard methods 5210D with oxitops. As a result of the
study, best removal performance observed in 100 mL Bacillus subtilis as 98% at 17 days in COD. In a soil study, 100 mL
mixture of Bacillus subtilis consortia used for bioremediation studies.
According to the results of artifical agricultural field study, the bioremediation
seen at COD and BOD₅ parameters are 99.4% and 96% respectively at
the end of 2. week. This experiments have focused that, Bacillus subtilis should be a selective type for bioremediation of
metribuzin on receiving environments.

Anahtar Kelimeler

Bacteria, bioremoval, herbicide, metribuzin

Kaynakça

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