Tarımsal Atık İçeren Katı Substrat Fermentasyonu Ortamında Pleurotus eryngii’nin Büyüme ve Şapka Oluşumu Esnasındaki Lakkaz Aktivitesi

Yıl 2015, Cilt: 1 Sayı: 1, 64 - 71, 07.09.2015

Numan Yildirim , Nuran Cikcikoglu Yildirim Abdunnasir Yildiz

Öz

Yenilebilir mantar Pleurotus eryngii de misel gelişim, primordiyum oluşumu ve
şapka oluşumu gibi farklı gelişim peryotlarındaki lakkaz aktivitesi üzerine farklı
tarımsal atıkların etkileri çalışılmıştır. Şapka oluşumu esnasında hem buğday samanı ve
hemde pamuk sapı içeren gelişim ortamında en yüksek lakkaz aktivitesi tespit
edilmiştir. Bu çalışmanın sonuçları lakkaz aktivitesinin mantarın şekilsel gelişiminde rol
oynadığını göstermiştir. Çalışmamızda fermentasyon ortamına farklı oranlardaki pirinç
kepeği katılmasının gelişim evrelerindeki lakkaz aktivitesini nasıl etkilediği de
çalışılmıştır. Yüksek oranda pirinç kepeği (%10) eklenmesinin gelişim peryotlarını
geciktirdiği tespit edilmiştir. Pirinç kepeğinin konsantrasyona bağlı olmakla beraber
gelişim evrelerinde lakkaz aktivitesini indüklediği tespit edilmiştir. Çalışmamızda P.
eryngii’nin iyi bir lakkaz üreticisi olduğu ve pirinç kepeğinin düşük konsantrasyonlarda
gelişim ortamına eklenmesinin lakkaz aktivitesini arttırdığını gösterdik.

Anahtar Kelimeler

Pleurotus eryngii, Lakkaz, Gelişim peryodu

Laccase Enzyme Activity during Growth and Fruiting of Pleurotus eryngii Under Solid State Fermentation Medium Containing Agricultural Wastes

Öz

The effect of different agricultural wastes and various growth periods such as mycelial growth, primordium formation, and fruit body yield on laccase activity in the edible mushroom Pleurotus eryngii (DC. ex Fr.) Quel. was studied. When the cap of the mature mushroom was showed, the highest laccase activity was obtained on both wheat straw and cotton stalk. Our results suggest that laccases may play a role in the morphogenesis of the mushroom. In this study, the effect of rice bran at various concentrations, which was previously added to fermentation conditions, on growth periods and laccase activity has also been investigated. It is determined that high concentration of rice bran (%10) delayed growth periods. Laccases were found to be induced by addition of rice bran in the medium but induction levels depends on the concentration. The results of the present study allow us to conclude that P. eryngii are good candidates for scale-up laccase production and rice bran at low concentration is a strong laccase-inducer

Anahtar Kelimeler

Pleurotus eryngii, Laccase, Growth period

Kaynakça

• Arora, D.S., Gill, P.K., 2001. Effects of various media and supplements on laccase production by some white rot fungi. Bioresour. Technol. 77: 89–91.
• Baysal, E., Peker, H., Yalinkilic, M.K., Temiz, A., 2003. Cultivation of oyster mushroom on waste paper with some added Bioresour. Technol., 89: 95–97.
• Birhanli, E., Yesilada, O., 2006. Increased production of laccase by pellets of Funalia trogii ATTC 200800 and Trametes versicolor ATCC 200801 in repeated-batch mode. Enzyme Microb. Tech., 39: 1286-1293.
• Bonnen, A.M., Anton, L.H., Orth, A.B., 1994. Lignin-degrading enzymes of the commercial Agaricus bisporus. Appl. Environ. Microb., 60: 960-965. mushroom,
• Chen, S., Ge, W., Buswell, J.A., 2004. Molecular cloning of a new laccase from the edible straw mushroom Volvariella involvement in fruit body development. FEMS Microbiol. Lett., 230: 171-176.
• Cohen, R., Persky, L., Hadar, Y., 2002. Biotechnological potential mushrooms of the genus Pleurotus. Appl. Microbiol. Biotechnol., 58: 582- 594. and of degrading
• Claye, S.S., Idouraine, A., Weber, C.W., 1996. Extraction of insoluble fiber from five sources. Food Chem., 57: 305-310.
• Demirci, Z., 1998. Utilization of non woody waste product for Pleurotus ostreatus cultivation in the region of Eastern Black Sea. M.Sc. thesis, Trabzon, Turkey.
• Eggert, C., Temp, U., Dean, J.F., Eriksson, K.E., 1996. A fungal metabolite mediates degradation of non-phenolic lignin structures and synthetic lignin by laccase. FEBS Lett., 391: 144-148.
• Gupta, Y., Vijay, B., 1991. Post composting supplementation in Agaricus bisporus under seasonal growing conditions. 13th International Congress of ISMS held at Dublin, Ireland.
• Glenn, J.K., Gold, M.H., 1983. Decolorization of several polymeric dyes by the lignin degrading Phanerochaete chrysosporium. Appl. Environ. Microb. 45: 1741-1747. Vidziunaite, Kulys, J., R., 2003. Amperometric biosensors based on recombinant laccases for phenols determination. Biosens. Bioelectron., 18: 319-325.
• Kahraman, S., Yesilada, O., 2001. Industrial and agricultural wastes as substrates for laccase production by white-rot fungi. Fol. Microbiol., 46: 133–6. Lechner, B.E., Papinutti, V.L., 2006. Production of lignocellulosic enzymes during growth and fruiting of the edible fungus Lentinus tigrinus on wheat straw. Process Biochem., 41: 594-598.
• Leatham, G.F., Kirk, T.K., 1983. Regulation of ligninolytic activity by nutrient nitrogen in white-rot basidiomycetes. FEMS Microbiol. Lett., 16: 65-67.
• Lalley, J.J., Janben, A., 1993. Productivity improvement of oyster mushroom substrate with a controlled release of nutrient. Mushroom News, 41: 6-13.
• Machuca, A., Ferraz, A., 2001. Hydrolytic and oxidative enzymes produced by white- and brown rot fungi during Eucalyptus grandis decay in solid medium. Enzyme Microb. Tech., 29: 386-391.
• Mayer, A.M., Staples, R.C., 2002. Laccase: new function of and old enzyme. Phytochemistry, 60: 551-565.
• Mester, T., Swarts, H.J., Sole, S.R.I., de Bont, J.A.M., Field, J.A., 1997. Stimulation of aryl metabolite production in the basidiomycete Bjerkandera sp. strain BOS55 with biosynthetic precursors and lignin degradation products. Appl. Environ. Microb., 63: 1987-1994.
• Mata, G., Savoie, J.M. 1998. Extracellular enzyme activities in six Lentinula edodes strains during cultivation in wheat straw. World J. Microb. Biot., 14: 513-519.
• Mod, R.R., Conkerton, E.J., Ory, R.O., Normand, F.L., 1978. Hemicellulose composition of dietary fiber of milled rice and rice bran. J. Agr. Food Chem., 26: 1031-1035. Nelson, D., Elisa, E., 2000. Potential applications of oxidative enzymes and phenoloxidase-like wastewater and soil treatment. Appl. Catal. B-Environ., 28: 83-99.
• Nelson, D., Maria, A.R., Alessandro, D., Liliana, G., 2002. Applications of laccases (phenoloxidases) different supports. Enzyme Microb. Technol., 31: 907-931. tyrosinases immobilized on
• Poppe J.A., Hofte, M., 1995. Twenty wastes for twenty cultivated mushrooms. In: Proceedings of the 14th International Congress Cultivation Rotterdam, pp. 171–179. and Fungi, of Edible
• Pratima, B., 1999. Application of enzymes in and Biotechnol. Progr., 15: 147-157. industry.
• Philippoussis, A., Zervakis, P., 2001. Diamantopoulou, Bioconversion lignocellulosic wastes through the cultivation of the edible mushrooms Agrocybe aegerita, Volvariella volvacea and Pleurotus spp. World. J. Microb. Biot., 17: 191–200.
• Rajarathnam, S, Wankhede, D.B., Bano, Z., 1987. Degradation of rice straw by Pleurotus Technol. Biotechnol., 37: 203-214. J. Chem.
• Swammy, J., Ramsay, J.A., 1999. Effect of glucose and NH4+ concentrations on sequential Trametes versicolor. Enzyme Microb. Tech., 25: 278-284.
• Srebotnik, E., Boisson, J.N., 2005. by Peroxidation of linoleic acid during the oxidation of phenols by fungal laccase. Enzyme Microb. Technol., 36: 785–9.
• Wong, Y., Yu, J., 1999. Laccase-catalysed degradation of synthetic dyes. Water Res., 33: 3512-3520.
• Xing, Z.T., Cheng, J.H., Tan, Q., Pan, Y.J., 2006. Effect of nutritional parameters on laccase production by the culinary and medicinal mushroom, Grifola frondosa. W. J. Mic. Biotech., 22: 1215-1221.
• Yildirim, N, Yildirim, N.C., Yildiz, A., 2010. Evaluation of three strains of Pleurotus eryngii isolated from geographically different regions of Turkey for their ability to decolorize methylen blue and chrystal violet. Fres. Environ. Bull., 19(3): 447-451.
• Yildiz, A., Karakaplan, M., 2003. Evalulation of some agricultural wastes for the cultivation Pleurotus ostreatus mushrooms: