Biodiversity of Bacteria Isolated from Different Soils

Year 2017, Volume: 4 Issue: 1, 27 - 34, 07.01.2017

Fatma Yaman Betül Aktaş Mustapha Touray , Esin Poyrazoğlu Çoban Hacı Bıyık

https://doi.org/10.21448/ijsm.278866

Cited By: 2

Abstract

The aim of this study was to determine the biodiversity of PHB producing
bacteria isolated from soils where fruit and vegetable are cultivated (onion,
grape, olive, mulberry and plum) in Aydın providence. Morphological, cultural,
biochemical, and molecular methods were used for bacteria identification. These
isolated bacteria were identified by 16S rRNA sequencing and using BLAST. The
following bacteria Bacillus thuringiensis
(6), Bacillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. The
Maximum Likelihood method was used to produce a molecular phylogenetic analysis
and a phylogenetic tree was constructed. These bacteria can produce
polyhydroxybutyrate (PHB) which is an organic polymer with commercial potential
as a biodegradable thermoplastic. PHB can be used instead of petrol derivated
non-degradable plastics. For this reason, PHB producing microorganisms are
substantial in industry.

Keywords

PHB, bacteria, 16S rRNA, biodiversity, soil

Biodiversity of Bacteria Isolated from Different Soils

Abstract

The aim of this study was to determine the biodiversity of PHB producing bacteria isolated from soils where fruit and vegetable are cultivated (onion, grape, olive, mulberry and plum) in Aydın providence. Morphological, cultural, biochemical, and molecular methods were used for bacteria identification. These isolated bacteria were identified by 16S rRNA sequencing and using BLAST. The following bacteria Bacillus thuringiensis (6), Bacillus cereus (8), Bacillus anthrachis (1), Bacillus circulans (1), Bacillus weihenstephanensis (1), Pseudomonas putida (1), Azotobacter chroococcum (1), Brevibacterium frigoritolerans (1), Burkholderia sp. (1), Staphylococcus epidermidis (1), Streptomyces exfoliatus (1), Variovorax paradoxus (1) were found. The Maximum Likelihood method was used to produce a molecular phylogenetic analysis and a phylogenetic tree was constructed. These bacteria can produce polyhydroxybutyrate (PHB) which is an organic polymer with commercial potential as a biodegradable thermoplastic. PHB can be used instead of petrol derivated non-degradable plastics. For this reason, PHB producing microorganisms are substantial in industry.

Keywords

PHB, bacteria, 16S rRNA, biodiversity, soil

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