An actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking conditions at 180 rpm. As a result of fermentation studies, the antagonistic effect of the crude extract, obtained through ethyl acetate extraction, against various microorganisms was determined. The MIC values of the extract against Staphylococcus aureus and Escherichia coli were 101.3 µg/mL and 153.6 µg/mL, respectively. It was also found to exhibit strong antifungal activity against Penicillium spp. Streptomyces sp. MC12, which displays both antifungal and antibacterial properties, is considered a potential secondary metabolite producer for future studies, particularly in pharmacology and the biocontrol of fungal pathogens.
Fermentation Identification Secondary metabolite Streptomyces sp. 16S rRNA sequencing
Project number: 2015-075
An actinobacterium newly isolated from soil during a screening study was identified as Streptomyces sp. MC12 (GenBank accession number: PP757795) based on 16S rRNA analysis. For secondary metabolite production, fermentation was carried out in ISP 2 broth at 30°C, pH 7.3, for seven days under shaking conditions at 180 rpm. As a result of fermentation studies, the antagonistic effect of the crude extract, obtained through ethyl acetate extraction, against various microorganisms was determined. The MIC values of the extract against Staphylococcus aureus and Escherichia coli were 101.3 µg/mL and 153.6 µg/mL, respectively. It was also found to exhibit strong antifungal activity against Penicillium spp. Streptomyces sp. MC12, which displays both antifungal and antibacterial properties, is considered a potential secondary metabolite producer for future studies, particularly in pharmacology and the biocontrol of fungal pathogens.
Fermentation Identification Secondary metabolite Streptomyces sp. 16S rRNA sequencing
Project number: 2015-075
Birincil Dil | İngilizce |
---|---|
Konular | Mikrobiyoloji (Diğer) |
Bölüm | Makaleler |
Yazarlar | |
Proje Numarası | Project number: 2015-075 |
Erken Görünüm Tarihi | 8 Ekim 2024 |
Yayımlanma Tarihi | 3 Kasım 2024 |
Gönderilme Tarihi | 9 Mayıs 2024 |
Kabul Tarihi | 30 Temmuz 2024 |
Yayımlandığı Sayı | Yıl 2024 Cilt: 11 Sayı: 4 |