Determination of Clopidogrel in Pharmaceutical Preparation by UV Spectrophotometry and High Performance Liquid Chromatography Methods

Yıl 2020, Cilt: 4 Sayı: 1, 14 - 19, 15.07.2020

Ömer Faruk Koçak , Yücel Kadıoğlu Onur Şenol

Öz

UV-Visible spectroscopy and high performance liquid chromatography (HPLC) methods were developed and validated for determination of clopidogrel in bulk and pharmaceutical formulations. In spectroscopy, clopidogrel spectrum at different solutions was taken and the best absorbance value was obtained at 202 nm wavelength in methanol-acetonitrile (50:50.h/h) mixture. In HPLC, parameters were optimized as follows: mobile phase was acetonitrile-methanol-water (45:45:10, v/v/v), C18 re-serve phase column was preferred, flow rate and injection volume were set as 0.9 mL/min and 10 μL, respectively. All measurements were performed at 230 nm wavelength. Meloxicam was used as an internal standard in HPLC. Linearity, analytic recovery, intra and inter-days precision and accuracy were investigated in order to per-form validation process, It was stated that HPLC measurements were linear at 0.25-30 μg/mL while UV-Visible method linear at 1.25-25 μg/mL. Intra and inter-days precision and accuracy values were 5.38% and 2.66% for HPLC method and 3.77% and 3.60% for UV-Vis. Mean analytic recovery value was found to be 99.5% for both methods. Developed and validated HPLC and UV-Vis methods were applied to pharmaceutical preparations including clopidogrel.

Anahtar Kelimeler

Clopidogrel, UV-Visible spectrophotometry, HPLC

Kaynakça

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