Botanical origin and antioxidant activities of propolis from the Irano-Turanian region

Yıl 2017, Cilt: 47 Sayı: 3, 107 - 111, 11.10.2017

İlginç Kızılpınar Temizer , Aytaç Güder , Ömür Gençay Çelemli

https://doi.org/10.5152/IstanbulJPharm.2017.0017

Öz

Propolis is a natural
bioactive mix and a traditional medicine that has been used for treating
several complications. The bioactive properties of propolis are dependent on
its botanical origin. This study investigated the pollen composition,
antioxidant activities, and the total phenol and total flavonoid content of a
propolis sample from the Refahiye (Erzincan, Turkey) region.
Melissopalynological analysis conducted according to the relevant literature
revealed that the pollen profile of the sample primarily indicated the presence
of the Fabaceae (38.4%), Asteraceae (20.2%), and Fagaceae (11.2%) families. The
antioxidant ability of propolis extract was analyzed by the hydrogen peroxide
scavenging activity (HPSA) (in terms of SC50), ferric reducing antioxidant
power capacity (FRAP) (%), DPPH radical scavenging activity (in terms of SC50),
metal-chelating activity (%), total phenol content (TPC), and total flavonoid
content (TFC), which showed the following values: 11.72±0.04 µg/mL, 90.73% ±
0.24%, 18.34±0.08
µg/mL, 89.69% ± 0.12%, 10673.4±3.30 mg GAE/100 g of propolis
sample (PS), and 170.65±1.12 mg QE/100 g of PS, respectively. These
results were compared using butylated hydroxyanisole (BHA), butylated
hydroxytoluene (BHT), and α-tocopherol (TOC) as standard antioxidant
compounds. The high biological activity of propolis from the Refahiye region
could be attributed to its rich pollen composition. These results indicate that
propolis is an important source in terms of its antioxidant activities.

Anahtar Kelimeler

Bioactive properties, mellisopalynological analysis, propolis, Refahiye (Erzincan)

Kaynakça

• Ahn M, Kumazawa S, Usui Y, Nakamura J, Matsuka M, Zhu F, Nakayama T (2007). Antioxidant activity and constituents of propolis collected in various areas of China. Food Chem 101: 1383–1392. Bankova VS, De Castro SL, Marcucci MC (2000). Propolis: Recent advances in chemistry and plant origin. Apidologie 31: 3–15.
• Blois MS (1958). Antioxidant determinations by the use of a stable free radical. Nature 181: 1199–1200.
• Borrelli F, Maffia P, Pinto L, Ianaro A, Russo A, Capasso F, Ialenti A (2002). Phytochemical compounds involved in the anti-inflammatory effect of propolis extract. Fitoterapia 73: 53-63.
• Choi YM, Noh DO, Cho SY, Suh HJ, Kim KM, Kim JM (2006). Antioxidant and antimicrobial activities of propolis from several regions of Korea. Lwt 39: 756–761.
• Chung YC, Chang CT, Chao WW, Lin CF, Chou ST (2002). Antioxidative activity and safety of the 50 ethanolic extract from red bean fermented by Bacillus subtilis Imr-Nk1. J Agr Food Chem 50: 2454–2458.
• Çelemli ÖG, Sorkun K (2012). The plant choices of honey bees to collect to propolis in Tekirdag-Turkey. Hacettepe J Biol & Chem 40: 45–51.
• Davis PH (ND). Flora of Turkey and the East Aegean islands. Edinburgh University Press, Edinburgh.
• Dinis TCP, Madeira VMC, Almeida LM (1994). Action of phenolic derivatives (Acetaminophen, Salicylate, And 5-Aminosalicylate) as inhibitors of membrane lipid peroxidation and as peroxyl radical scavengers. Arch Biochem Biophys 315: 161–169.
• Geckil H, Ates B, Durmaz G, Erdogan S, Yilmaz I (2005). Antioxidant, free radical scavenging and metal chelating characteristics of propolis. AM J Biochem and Biotech 1: 27-31.
• Gençay Ö, Sorkun K (2006). Microscopic analysis of propolis samples collected from East Anatolia (Kemaliye-Erzincan). FABAD J Pharm Sci 31: 192-197.
• Güder A, Korkmaz H, Gökce H, Alpaslan YB, Alpaslan G (2014). Isolation, characterization, spectroscopic properties and quantum chemical computations of an important phytoalexin resveratrol as antioxidant component from Vitis labrusca L. and their chemical compositions. doi: 10.1016/j.saa.2014.05.056.
• Gülçin İ, Bursal E, Şehitoğlu MH, Bilsel M, Gören AC (2010). Polyphenol contents and antioxidant activity of lyophilized aqueous extract of propolis from Erzurum, Turkey. Food Chem Toxicol 48: 2227–2238.
• Hegazi AG, Abd El Hady FK (2001). Egyptian propolis: 1-Antimicrobial Activity And chemical composition of upper Egypt propolis. Z Naturforsch C 56: 82–88.
• Herrera CL, Alvear M, Barrientos L, Montenegro G, Salazar LA (2010). The antifungal effect of six commercial extracts of Chilean propolis on Candida spp.. Cien Inv Agr 37: 75–84.
• Huang S, Zhang CP, Wang K, Li G, Hu FL (2014). Recent advances in the chemical composition of propolis. Molecules 19: 19610–19632.
• Kalogeropoulos N, Konteles SJ, Troullidou, E, Mourtzinos I, Karathanos VT (2009). Chemical composition, antioxidant activity and antimicrobial properties of propolis extracts from Greece and Cyprus. Food Chem 116: 452–461.
• Kumazawa S, Hamasaka T, Nakayama T (2004). Antioxidant activity of propolis of various geographic origins. Food Chem 84: 329–339.
• Laskar RASI, Roy N, Begum NA (2010). Antioxidant activity of Indian propolis and its chemical constituents. Food Chem 122: 233–237.
• Lima B, Tapia A, Luna L, Fabani MP, Schmeda-Hlrschmann G, Podio NS, Feresin GE (2009). Main flavonoids, dpph activity, and metal content allow determination of the geographical origin of propolis from the province of San Juan (Argentina). J Agr Food Chem 57: 2691–2698.
• Marcucci MC (1995). Propolis: Chemical composition, biological properties and therapeutic activity. Apidologie 26: 83–99.
• Moreira L, Dias LG, Pereira JA, Estevinho L (2008). Antioxidant properties, total phenols and pollen analysis of propolis samples from Portugal. Food Chem Toxicol 46: 3482–3485.
• Oršolić N, Bašić I (2003). Immunomodulation by water-soluble derivative of propolis: A factor of antitumor reactivity. J Ethnopharmacol 84: 265–273.
• Ota C, Unterkircher C, Fantinato V, Shimizu MT (2001). Antifungal activity of propolis on different species of Candida. Mycoses 44: 375–8.
• Özhatay N, Kültür Ş, Gürdal B (2013). Check-List Of Additional Taxa To The Supplement Flora Of Turkey VI. J Fac Pharm Istanbul 43: 33–82.
• Perveen A, Qaiser M (2007). Pollen morphology of family Solanaceae from Pakistan. Pakistan J Bot 39: 2243–2256.
• Pontis JA, Da Costa LAMA, Da Silva SJR, Flach A (2014). Color, phenolic and flavonoid content, and antioxidant activity of honey from Roraima, Brazil. Food Sci Tech-Brazil 34: 69–73.
• Popova M, Silici S, Kaftanoglu O, Bankova V (2005). Antibacterial activity of Turkish propolis and its qualitative and quantita- tive chemical composition. Phytomedicine 12: 221–228.
• Ruch RJ, Cheng SJ, Klaunig JE (1989). Prevention of cytotoxicity and inhibition of intercellular communication by antioxidant catechin isolated from Chinese green tea. Carcinogenesis 10: 1003–1008.
• Sawicka D, Car H, Borawska MH, Nikliński J (2012). The anticancer activity of propolis. Folia Histochem Cytobio 50: 25-37.
• Sforcin JM, Fernandes A, Lopes CAM, Bankova V, Funari SRC (2000). Seasonal effect on Brazilian propolis antibacterial activity. J Ethnopharmacol 73: 243–249.
• Silva BB, Rosalen PL, Cury JA, Ikegaki M, Souza VC, Esteves A, Alencar SM (2008). Chemical composition and botanical origin of red propolis, a new type of Brazilian propolis. Evid-Based Compl Alt 5: 313–316.
• Silva IAA., Da Silva TMS, Da Camara CA, Queiroz N, Magnani M, Novais JS, De Souza A GDe. (2013). Phenolic profile, antioxidant activity and palynological analysis of stingless bee honey from Amazonas, Northern Brazil. Food Chem 141: 3252–3258.
• Slinkard K, Singleton V (1977). Total phenol analysis: automation and comparison with manual methods. Amer Soc Enology Viticulture 28: 49–55.
• Sobočanec S, Balog T, Šari A, Mačak-Šafranko Ž, Štroser M, Žarković K, Marotti T (2011). Antitumor effect of Croatian propolis as a consequence of diverse sex-related dihydropyrimidine dehydrogenase (dpd) protein expression. Phytomedicine 18: 852–858.
• Wagh VD (2013). Propolis: A wonder bees product and its pharmacological potentials. Advances in Pharmacological Sciences 23: 1-11.
• Wali AF, Avula B, Ali Z, Khan IA, Mushtaq A, Rehman MU, Masoodi MH (2015). Antioxidant, hepatoprotective potential and chemical profiling of propolis ethanolic extract from Kashmir Himalaya region using Uhplc-Dad-Qtof-Ms. doi: 10.1155/2015/393462.
• Warakomska Z, Maciejewic W (1992). Microscopic analysis of propolis from Polish regions. Apidologie 23: 277-283