Determination of chlorpheniramine enantiomers in pharmaceutical formulations by HPLC on chiral column with PDA detection

Year 2020, Volume: 50 Issue: 2, 149 - 152, 27.08.2020

Gizem Erensoy Duygu Taşkın Gamze Özgül Artuç , Elif Özdemir Sumru Özkırımlı

Abstract

Background and Aims: An HPLC method with photodiode array detector on a chiral column was proposed for enantioselective determination of chlorpheniramine (CLP) enantiomers in dosage forms. Methods: The enantioselective determination was achieved on amylose tris(3,5-dimethylphenylcarbamate) column, using n-hexane-(propan-2-ol)-diethylamine (97.5:2.5:0.025, v/v/v) mobile phase. The peaks were detected at 258 nm. Diphenhydramine was used as an internal standard (IS). A new sample preparation procedure was developed to avoid the interference of the other ingredients present in the formulations. Results: Limit of quantification of the proposed method was 0.88 and 1.31 μg/mL for S-(+)-CLP and R-(-)-CLP, respectively. Conclusion: The method is linear, sensitive, specific and can be used for the enantioselective assay of CLP enantiomers in pharmaceutical formulations.

Keywords

Chlorpheniramine, amylose tris(3,5-dimethyl phenylcarbamate), enantioselective determination, HPLC-PDA, chirality

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