Kısraklarda İntrafallopian Gamet Transferi

Yıl 2010, Cilt: 36 Sayı: 2, 53 - 61, 22.12.2010

Mehmet Gündüz

Öz

Kısraklarda oosit eldesi ve transferi teknikleri son yıllarda oldukça ilerlemiştir. Bu ilerlemeyle birlikte yeni araştırma ve klinik uygulama olanakları da ortaya çıkmıştır. Ovidukt, uterus ve serviks problemleri yüzünden gebe kalmayan ya da gebeliklerini devam ettiremeyen kısrakların oosit transferiyle fertiliteleri devam ettirilebilmektedir. Oositler genellikle ovulasyona yakın folliküllerden transvaginal ultrason rehberliğinde toplandıktan sonra ya direkt alıcı kısrağın oviduktuna transfer edilir ya da kısa bir süre kültür ortamında bırakılır. Alıcı kısrakların fertilizasyonu ya doğal aşımla ya da intrauterin suni tohumlamayla sağlanır. Sınırlı sayıda sperm olduğu durumlarda ise oosit ve sperm birlikte ovidukta transfer edilir.

Anahtar Kelimeler: Gamet transferi, oosit, IVF, kısrak

 

 

 

ABSTRACT

GAMETE INTRAFALLOPIAN TRANSFER (GIFT) IN MARES

 

Methods for the collection and transfer of equine oocytes have been developed, and uses of these techniques have resulted in new clinical and research possibilities. Because oocyte transfer avoids reproductive problems associated with the oviduct, uterus, and cervix, pregnancies can be produced from many mares that can not carry a pregnancy or produce embryos. Oocytes for clinical transfers are usually collected from preovulatory follicles by using transvaginal ultrason-guided follicular aspiration and cultured for a short interval or transferred directly into a recipient's oviduct. For oocyte transfer, the recipient is inseminated within the uterus or served naturally. Sperm and oocyte are transferred into the oviduct where sperm numbers are limited.

Key Words: Gamete transfer, oocyte, IVF, mare

Anahtar Kelimeler

Gamet transferi, oosit, IVF, kısrak

Kaynakça

• Allen, W.R., Rowson, L.E.A., 1972. Surgical and non-surgical egg transfer in horses. In:Prooceedings of the 7t h International Congress on Animal Reproduction and Artificial Insemination, Munich, Germany, pp. 484-487.
• Bezard, J . , Magistrini, M., Duchamp, G., 1989. Chronology of equine fertilization and embryonic devolopment in vivo and iv vitro. Equine Vet J.(Suppl.) 8,105-110.
• Bezard, J . , Palmer, E . , 1992. In vitro maturation old horse oocytes from slaugtered ovaries. In: Prooceedings of the 12th International Congress of Animal Reproduction, The Hague, The Netherlands p.315.
• Bracher, V., Parlavliet, J . , Fazeli, A.R., 1993. Repeated trasvaginal ultrasound-guided follicular aspiration in the mare. Equine Vet J.(Supp) 15, 75-79.
• Bruck, I., Raun, K., Synnestvedt, B., 1992. Follicule aspiration in the mare using transvaginal ultrasound guided technique. Theriogenology 24,58-59.
• Carnevale, E . M . , Ginther, O.J., 1993. Use of a linear ultrasonic transducer for the transvaginal aspiration and transfer of oocytes in the mare. J. Equine Vet. Sci. 13, 331-333.
• Carnevale, E.M., Griffin, P.G., Ginther, O.J., 1993. Age-associated subfertility before entry of embryos into the uterus in mares. Equine Vet. J. Suppl. 15, 31-35
• Carnevale, E . M . , Ginther, O.J., 1995. Defective Oocytes as a Cause of Subfertility in Old Mares. Biol Reprod Mono. 1, 209-214.
• Carnevale, E . M . , 1996. Gamete Intrafollopian Transfer. Veterinary Clinics of North America: Equine Practice.; 12(1),47-60.
• Carnevale, E.M., Uson, M., Bozzola, J.J., King, S.S., Schmitt, S.J., Gates, H.D., 1999. Comparison of ooyctes from young and old mares with light and electron microscopy. Theriogenology 51, 299-305.
• Carnevale, E.M., 2004. Oocyte transfer and gamete intrafallopian transfer in the mare. Animal Reproduction Science 82, 617-624.
• Choi, Y . H . , Chung, Y . G . , Seidel, G . E . , Squires, E . L . , 2001. Developmental capacity of equine oocytes matured and cultured in equine trophoblast-conditioned media. Theriognology 56, 329-339.
• Cook, N.L., Squires, E . L . , Ray, B.S., Cook, V.M., Jasko, D.J., 1993. Transvaginal ultrasonically -guieded follicular aspiration of equine oocytes. Equine Vet J.(suppl.) 15, 71-74.
• Coutinho da Silva, M.A., 2008. When should a mare go for assisted reproduction? Theriogenology. 70(3), 441-444.
• Ginther, O.J., 1992. Reproductive biology of the mare, ed. 2. Cross Planins, WI, Equiservices.
• Hinrichs, K., Kenney, D.F., Kenney, R.M., 1990. Aspiration of oocytes from mature and immature preovulatory follicles in the mare. Theriogenology 34, 107-112.
• Jacobson, C.C., Choi, Y.H., Hayden, S.S., Hinrichs, K., 2010. Recovery of mare oocytes on a fixed biweekly schedule, and resulting blastocyst formation after intracytoplasmic sperm injection. Theriogenology. 73(8), 1116-1126.
• McKinnon, A.O., Carnevale, E.M., Squires, E . L . , Voss, J . L . , Seidel Jr., G . E . , 1988. Heterogenous and xenogenous fertilization of in vivo matured equine oocytes. J. Equine Vet. Sci. 8, 143-147.
• McKinnon, A.O., Squires, E . L . , 1988. Equine embryo transfer. Vet Clin North Am: Equine Prac. 4, 305-333.
• Meintjes, M., Bellow, E.S., Paul, J.B., 1995. Trasvaginal ultrasound guided oocyte retrieval in cyclic and pregnant horse and pony mares for in vitro fertilization. Biol prerod Monograph Series. 1, 281-287.
• Nelson, J R , Corson, S L , Batzer, F R , Gocial, B, Huppert, L , Go, K J , Maislin, G., 1993. Predicting succes of gamete intrafallopian transfer. Fertl. Steril. 60, 116-122.
• Pieterse, M.C., Kappen, K.A., Kruip, T.A.M., 1988. Aspiration of bovine oocytes during transvaginal ultrasound scanning of the ovaries. Theriogenology 37, 751-768.
• Ransom, MX, Corsan, GH, Garcia, A J , Doherty, KA, Kemmann, E . , 1994. Tubal selection for gamete intrafallopian transfer. Fertl. Steril.; 61, 386-389.
• Rantanen, N.W., McKinnon, A.O., 1998. Equine Diagnostic Ultrasonography. Willams and Wilkins.
• Squires, E . L . , Barnes, C.K., Rowley, H.S., 1989. Effect of uterine fluid and volume of extender on fertility. In: Proocedings of the 35t h Annual Convention of the American Association of Equine Practitioners, Boston, M A pp: 25-30.
• Squires, E . L . , Cook, N.L., 1996. Transvaginal Aspiration. Veterinary Clinics of North America: Equine Practice. 12(1), 13-29.
• Kisraklarda Gamete Intro Fallopian Transfer (Gift)
• Squires, E . L . , 1996. Maturation and fertilization of equine oocytes. Veterinary Clinics of North America: Equine Practice. 12(1), 31-45.
• Squires, E . L . , Cook N.L., 1998. Ultrasound-Guided Folicular Aspiration. In: Equine Diagnostic Ultrasonography Eds: Rantanen NW,McKinnon AO. Williams & Wilkins Maryland pp: 213-220.
• Squires, E . J . , Carnevale, E . M., McCue, P. M., Bruemmer, J . E . , 2003. Embryonic technologies in the horse. Theriogenology 51, 151-170.
• Vogelsang, M.M., Kreider, J . L . , Bowen, M.J., 1988. Methods for collecting follicular oocytes from mares. Theriogenology 29, 1007-1018.