Current perspectives on the Nutrient composition and health benefits of yams (Discorea species)

Yıl 2021, , 179 - 190, 28.06.2021

Phılıppa Ojımelukwe , Chijioke Muoasinam Rachael Omodamiro

https://doi.org/10.31015/jaefs.2021.2.7

Cited By: 1

Öz

Yams (Discorea spp) are food security crops which serve as invaluable food sources of energy, micronutrients and phytochemicals with numerous health benefits. They show a lot of genetic and compositional diversity. New biologically active compounds are being identified from wild yams. This review focuses on the nutrients, and phytochemicals found in yam species and their health benefits. The B group of vitamins, vitamin C, pro-vitamin A; copper; manganese; isoflavones; steroidal saponins; alkaloids; terpenes; and anthocyanidins are found in different species of yams. Wild yams such as Discorea villosa and Discorea hispida are used in ethnomedicine because of their phytochemical content. Diosgenin, dioscorin, dioscin dihydrodiscorine protodioscin, methyprotodioscin, prosapogenin, epifzelechin, glucopyranoside, phytoestrogen and allantoin are bioactive phytochemicals that may be found in yams. They are valuable as anti-cancer, anti-mutagenic, anti-inflammatory, anti-diabetic, anti-ageing lipid lowering and hepatoprotective agents. Other claims based on traditional uses of yams have not been scientifically substantiated. Dioscorea species are likely to contain other unidentified secondary metabolites. Discorea species with other health promoting bioactive compounds should be identified; modern techniques should be used to extract yet unidentified compounds in Discorea and the food use and health benefits of yam species should be optimized.

Anahtar Kelimeler

Yams, Composition, Nutrients, Phytochemicals

Destekleyen Kurum

MICHAEL OKPARA UNIVERSITY OF AGRICULTURE, UMUDIKE, NIGERIA

Proje Numarası

NONE

Kaynakça

• Accatino, L., Pizarro, M., Solis, N., and Koenig C. (1998). Effects of diosgenin, a plant derived steroid, on bile secretion and hepatocellular cholestasis induced by estrogens in the rat. Hepatology. 28 (1):129-140. Doi:https://doi.org/10.1002/hep.510280118
• Achi, O.K., and Akubor, P.I. (2000). Microbiological characterization of yam fermentation for Elubo (yam flour) production. World J Microbiol Biotech. 16 (37): 1-7 Doi: https://doi.org/10.1023/A:1008980912708
• Adedayo, B. C., Ademiluyi, A.O., Oboh, G., and Akindahunsi, A.A. (2012). Interaction of aqueous extracts of two varieties of yam tubers (Discorea spp) on some key enzymes linked to type 2 Diabetes in vitro. Int J Food Sci Technol. 49:703-709. Doi: https://doi.org/10.1111/j.1365-2621.2011.02896.x
• Adepoju, O.T., Boyejo, O and Adeniji, P.O. (2017). Nutrient and antinutrient composition of yellow yam (Discorea cayenensis). Products Data in brief 11:428-431.
• Ali, Z., Smillie, T.J., and Khan, I.A. (2013) Cholestane steroid glycosides from the rhizomes of Dioscorea villosa (wild yam). Carbohydr Res. 370:86-91. Doi: https://doi.org/10.1016/j.carres.2012.12.022
• Aumsuwan, P., Khan, S.I., Khan, I.A., Avula, B., Walker, L.A., Helferich, W.G., Katzenellenbogen, B.S., and Dasmahapatra, A.K. (2015). Evaluation of wild yam (Dioscorea villosa) root extract as a potential epigenetic agent in breast cancer cells. In Vitro Cell Dev Biol Anim. 51(1):59-71. Doi: https://doi.org/10.1007/s11626-014-9807-5
• Aumsuwan, P.1., Khan, S.I., Khan, I.A., Ali, Z., Avula, B., Walker, L.A., Shariat-Madar, Z., Helferich, W.G., Katzenellenbogen, B.S., and Dasmahapatra, A.K. (2016).The anticancer potential of steroidal saponin, dioscin, isolated from wild yam (Dioscorea villosa) root extract in invasive human breast cancer cell line MDA-MB-231 in vitro. Arch Biochem Biophys. 1; 591:98-110. Doi: https://doi.org/10.1016/j.abb.2015.12.001. Epub 2015 Dec 10.
• Barton, H. (2014). Yams: origins and development. In: Encyclopaedia of Global Archeology, p 7943-7947 (Springer. Doi: https://doi.org/10.1007/978-1-4419-0465-2_2193
• Bevan, C. W. L and Hirst, J. (I985). A Convulsant Alkaloid of Dioscorea dumetorum, Chem Ind 4: 103. Doi: https://doi.org/10.1038/177935a0 Bhandari, M.R., Kasai, T., and Kwabata, J. (2003). Nutritional evaluation of wild yam (Discorea spp) tubers of Nepal. Food Chem. 82:619-623. Doi: https://doi.org/10.1016/S0308-8146(03)00019-0
• Bhandari, M.R., and Kwabata, J. (2004a). Assessment of antinutritional factors and bioavailability of calcium and zinc in wild yam (Discorea spp) tubers of Nepal. Food Chem. 85 (2) 281-287. Doi: https://doi.org/10.1016/j.foodchem.2003.07.006
• Bhandari, M.R., and kwabata, J. (2004b). Organic acid, phenolic content and antioxidant activity of wild yam (Discorea spp) tubers of Nepal. Food Chem. 88:163-168. Doi: https://doi.org/10.1016/j.foodchem.2003.12.027
• Bhandari, M.R., and kwabata, J. (2005). Bitterness and toxicity in wild yam (Discorea spp) tubers of Nepal. Plant Foods Hum Nutr. 60:129-135. Doi: https://doi.org/10.1007/s11130-005-6841-1
• Boban, P.T., Nambisan, B., and Sudhakaran, P.R. (2006) Hypolipidaemic effect of chemically different mucilages in rats: a comparative study. Br J Nutr. 96 (6):1021-1029. Doi: https://doi.org/10.1017/BJN20061944
• Bone, K., and Mill, S. (2000) eds. Principles and Practices of Phytotherapy, Modern Herbal Medicine. London: Churchill Livingstone
• Cai J, Liu M, Wang Z, and Ju Y. (2002). Apoptosis induced by dioscin in Hela cells. Biol Pharm Bull. 25 (2):193–196 Doi: https://doi.org/10.1248/bpb.25.193
• Carroll, D.G. (2006) Non-hormonal therapies for hot flashes in menopause. Am Fam Physician. 73(3):457-464.
• Chandrasekara, A., and Kumar, T.J. (2016). Roots and tuber crops as functional foods: A review on phytochemical constituents and their potential health benefits. Int J Food Sci. 1-15. Doi: https://doi.org/10.1155/2016/3631647
• Chang, W.C., Yu, Y.M., Wu, CH., Tseng, Y.H., and Wu, K.Y. (2005). Reduction of oxidative stress and atherosclerosis in hyperlipidemic rabbits by Dioscorea rhizome. Can J Physiol Pharmacol. 83 (5):423-430. Doi: https://doi.org/10.1139/y05-028
• Cho, J., Choi, H., Lee, J., Kim, M.S., Sohn, H.Y., and Lee, D.G. (2013). The antifungal activity and membrane-disruptive action of dioscin extracted from Dioscorea nipponica. Biochim Biophys Acta. 1828 (3):1153-1158. Doi: https://doi.org/10.1016/j.bbamem.2012.12.010
• Chukwu, I., G.O., Ezenwa, M.I., Osunde, A., Asiedu, R., and Ogbogu, N. J. (2007). Qualities of yam tubers grown on typic paleudults: Hybrid yam and fertilizer effects. Science Resource Essay, 2(12): 508-511.
• Debnath, M.K. (2019). Exploring of greater yam (Dioscorea alata L.) genotypes through biochemical screening for better cultivation in south Gujarat zone of India. Physiology and Molecular Biology of Plants. 1-15. Doi: https://doi.org/10.1007/s12298-019-00698-w
• Dong, S, H., Nikolic, D., Simmer, C., Qiu, F., van Breemen, R.B.,Djaja, D., Soejarto G.F., and Chen, S.N. (2012). Diarylheptanoids from Dioscorea villosa (Wild Yam) J Nat Prod. 75 (12), 2168-2177. Doi: https://doi.org/10.1021/np300603z
• Dong, M., Meng, Z., Kuerban, K, Qi F, Liu J, Wei Y, Wang Q, Jiang S, Feng M, Ye, L.  (2018) Diosgenin promotes antitumor immunity and PD-1 antibody efficacy against melanoma by regulating intestinal microbiota. Cell Death Dis 9, 1039. Doi: https://doi.org/10.1038/s41419-018-1099-3
• Egbonu, A.C.C., and Nzewi, D. (2016). Influence of blanching on some nutrient and anti-nutrient compositions of Bitter yam (Discorea dumetorum). British J Appl Sci Tech 16 (2):1-8. Doi: https://doi.org/10.9734/BJAST/2016/25650
• Ezeabara, C.A., and Anona, R. (2018). Comparative analysis of phytochemical and nutritional compositions of four species of Discorea. Acta Scientific Nutr Health 2.7:90-94
• Ezeocha V.C and Ojimelukwe, P.C. (2012). The impact of cooking on the proximate composition and anti-nutritional factors of water yam (Dioscorea alata). Journal of Stored Products and Post-Harvest Research. 3 (13) 172-176.
• Fanyi, M.A., Wang, R., Zhu, J., Zhang, Y., Wang, Y., Hu, W., Bell, A.E., and Liu, X. (2019). Characterization, comparison of polysaccharides from Discorea opposita. Thnub. Growing in sandy soil, loessial soil and continuous cropping. Int J Biol Macromols 126:776-785. Doi: https://doi.org/10.1016/j.ijbiomac.2018.12.259
• FAO. (2008). Food and Agricultural Organization of the United Nations. FAO Statistics 2009. FAO Rome. Retrieved from http://faostat.fao.org/
• FAOSTAT (2020). Food and Agricultural Organization of the United Nations. FAO Statistics 2020. FAO Rome. Retrieved from http://faostat.fao.org/
• Ferede, R., Maziya-Dixon, B., Oladeji, E.A., and Asiedu, A. (2010). Identification and quantification of major carotenoids of deep yellow-fleshed (tropical Discorea dumetorum). J Food, Agric Environ 8 (3&4):160-166. DOI: https://doi.org/10.1234/4.2010.2992.
• Foster, S., and Tyler, V.E. (2000). Tyler’s Honest Herbal. Binghamton, NY: The Haworth Herbal Press; 381-382.
• Ghezali, L., Liagre, B., Limami, Y., Beneytout, J. L. and Leger, D. Y. (2014) Sonic hedgehog activation is implicated in diosgenin-induced megakaryocytic differentiation of human erythroleukemia cells. PLoS ONE 9, e95016. Doi: https://doi.org/10.1371/journal.pone.0095016
• Go, H.K., Rahman, M.M., Kim, G.B., Na, C.S., Song, C.H., Kim, J.S., Kim, S.J., and Kong, H.S. (2015). Antidiabetic effect of yam (D.batatas) in a rat model of streptozotocin-induced diabetic rats. Nutrients 7:8532-8544. Doi: https://doi.org/10.3390/nu7105411
• Guo Y, Xing E, Song H, Feng G, Liang X, An G, Zhao X, Wang M. (2016) Therapeutic effect of dioscin on collagen-induced arthritis through reduction of Th1/Th2. Int Immunopharmacol. 39:79–83. Doi: https://doi.org/10.1016/j.intimp.2016.06.029
• Guilois, S., Lahol, M.C., and Dafour, D. (2011). Physicochemical, functional and macromolecular properties of waxy yam starches discovered from “Mapuey” (Discorea trifida) genotypes in the Venezuela Amazon natural amylose-free starch. J Agric Food Chem 59 (1):263-273. Doi: https://doi.org/10.1021/jf100418r
• Haimov-Kochman, R., and Hochner-Celnikier, D. (2005) Hot flashes revisited: pharmacological and herbal options for hot flashes management. What does the evidence tell us? Acta Obstet Gynecol Scand. 84 (10):972-9. Doi: https://doi.org/10.1111/j.0001-6349.2005.00769.x
• Harijono, T.E., Apriliyanti, M.W., Afriliana, A., and Kusnadi, J. (2013) Physicochemical and bioactives characteristics of purple and yellow water yam (Dioscorea alata) tubers. Int J PharmTech Res;5(4):1691-1701. Retrieved from: http://sphinxsai.com/2013/OD/PharmOD1
• Hou WC, Lee MH, Chen HJ, et al. Antioxidant activities of dioscorin, the storage protein of yam (Dioscorea batatas Decne) tuber. J Agric Food Chem. 2001; 49 (10):4956–4960. Doi: https://doi.org/10.1021/jf010606m
• International Institute of Tropical Agriculture (IITA) (2015) Yam genetic resources conserved at IITA. IITA R4D review issue 11. Retrieved from http://www.iita.org/r4dreview
• Iwu MM, Okunji CO, Akah P, Tempesta M.S, and Corley D. (1990) Dioscoretine: the hypoglycemic principle of Dioscorea dumetorum.Planta Med. 56 (1):119-20. Doi: https://doi.org/10.1055/s-2006-960901
• Kelley, K.W., and Carroll, D.G. (2010) Evaluating the evidence for over-the-counter alternatives for relief of hot flashes in menopausal women. J Am Pharm Assoc 50 (5):e106-115. Review. Doi: https://doi.org/10.1331/JAPhA.2010.09243
• Komesaroff, P.A., Black, C.V.S., Cable, V and Sudhir, K. (2009). Effects of wild yam extract on menopausal symptoms, lipids and sex hormones in healthy menopausal women. Climateric 4 (2) 144-150. Doi: https://doi.org/10.1080/cmt.4.2.144.150
• Kumar, S., Das, G., Shin, H.S and Patra, J.K. (2017). Discorea spp (A wild edible tuber): A study on its ethnopharmacological potential and traditional use by the local people of Similipal Bioshpere Reserve India. Frontiers Pharmacol 8:52:1-17. Doi: https://doi.org/10.3389/fphar.2017.00052
• Kumoro, A.C., and Hartati, I. (2015). Microwave assisted extraction of dioscorin from Gadung (Discorea hispida Dennst) tuber flour. Procedia Chem. 14:47-55. Doi: https://doi.org/10.1021/jf0203287
• Lin, J.T.,and Yang, D.J. (2008). Determination of steroidal saponins in different organs of yam (Discorea pseudojaponica Yamamoto). Food Chem 108:1068-1074. Doi: https://doi.org/10.1016/j.foodchem.2007.11.041
• Lippert, C., Seeger, H., and Mueck, A.O. (2003). The effect of endogenous estradiol metabolites on the proliferation of human breast cancer cells. Life Sci.72:877-883. Doi: https://doi.org/10.1016/s0024-3205(02)02305-6
• Manda, V.K., Avula, B., Ali, Z., Wong, Y.H., Smillie, T.J., Khan, I.A, and Khan, S.I. (2013) Characterization of in vitro ADME properties of diosgenin and dioscin from Dioscorea villosa. Planta Med. 79:1421-1428. Doi: https://doi.org/10.1055/s-0033-1350699
• Miyazawa, M., Shimamura, H., Nakamura, S., Kameoka, H. (1996). Anti-mutagenic activity of (+) – β- Eudesmol and Paenol from Discorea japonica. J Agric Food Chem 44:1647-1650. Doi: https://doi.org/10.1021/jf950792u
• Muthamia, Z. K., Morage F. E., Nyende A. B., Mamati E. G., Wanjala B. W. (2013). Estimation of the genetic diversity of the Kenyan yam (Dioscorea spp.) using microsatellite markers. African J Biotech. 12 (40): 5845-5841. Doi: https://doi.org/10.5897/AJB2013.12362
• Mignouna, H. D., Dansi A., Zok S. (2002) Morphological and isozymic diversity of the cultivated yams (Dioscorea cayenensis/Dioscorea rotundata complex) of Cameroon. Genetic Resources and Crop Evolution. 49 21–29. Doi: https://doi.org/10.1023/A:1013805813522
• Mc Anuff, M. A., Harding, W. W., Omoruyi, F. O., Jacobs, H., Morrison, E. Y., & Asemota, H. N. (2003). Alterations in intestinal morphology of streptozotocin induced diabetic rats fed Jamaican bitter yam (Dioscorea polygonoides) steroidal sapogenin extract. Nutr Res, 23, 1569-1577. Doi: http://dx.doi.org/10.1016/S0271-5317(03)00163-5
• McPherson, E., Jane, J. (1999). Comparison of waxy potato with other root and tuber starches. Carbohydrate Polymers. 40: 57-70. Doi: https://doi.org/10.1016/j.foodchem.2008.05.046
• Meleady, P., Clynes, M. (2001). Bromodeoxyuridine increases keratin 19 protein expression at a posttranscriptional level in two human lung tumor cell lines. In Vitro Cellular & Dev Biol - Animal 37 (8):536-42. Doi: https://doi.org/10.1290/1071-2690(2001)037<0536:BIKPEA>2.0.CO;2
• Nweke, F.I. (2016). Yam in West Africa, Food, Money and More. Michigan State University Press, East Lansing USA. Pp112-114
• Ojimelukwe, P.C., Ukom, A.N. 2017. Microbial and Antioxidant Activities of Some Common Spices from Southeast Nigeria. J Appl Life Sci Int. 13 (4) 1-10 Doi: https://doi.org/10.9734/JALSI/2017/3622791
• Ojo, O. I., Ojo, J. O. (2009). A comparative study of Effects of storage on Basic Nutritional composition of two Major Edible Dioscorea dumentorum varieties. Global J. Pure Appl. Sc., 15 (3&4), 353-355. Doi: http://dx.doi.org/10.4314/gjpas.v15i3-4.48553
• Oladeji, A.E., Maziya-Dixon, B., Federe- Menko, R., Popoola I., Asiedu, R., Gondwe, T. (2016). Characterization and classification of the pro vitamin A carotenoids of deep yellow-fleshed bitter yam (Discorea dumetorum) varieties. J Food Nutr Res. 4 (10):640-645. Doi: https://doi.org/10.12691/jfnr-4-10-2
• Oben, J.A., Egbe, A. E., Chuyong, G.B., Tabot, P.T. (2016). Diversity of yam (Discorea spp) populations in South-Western region of Cameroon. American J Life Sci 4 (6):187-194. Doi: https://doi.org/10.11648/j.ajls.20160406.17
• Obidiegwu, J.E., Akpabio, E.M. (2017). The geography of yam cultivation in Southern Nigeria: Exploring its social meanings and cultural functions. J Ethnic Foods 4:28-35. Doi: https://doi.org/10.1016/j.jef.2017.02.004
• Ojimelukwe, P.C., Asumugha, V.U. and Omeire, G.C. 2005. Fundamentals of Food Science and Nutrition. ISBN 1975-170096. (Bells Books)
• Okoye, B.C., Aido, R., Nweke, F. (2013). Yam consumption patterns in West Africa. Technical report. Doi: https://doi.org/10.13140/RG2218917.50401
• Park MK, Kwon HY, Ahn WS, Bae S, Rhyu MR, Lee Y. (2009) Estrogen activities and the cellular effects of natural progesterone from wild yam extract in mcf-7 human breast cancer cells. Am J Chin Med.37 (1):159-167. Doi: https://doi.org/10.1142/S0192415X09006746
• Patel, KS., Karmakar N., Desai, K. D., Narwande, Ajay V. N, Gayacharan Chakravarty, G., Tholl D. (2015). Biosynthesis and Biological Functions of Terpenoids in Plants. Biotechnology of Isoprenoid spp 63-106. In: Advances in Biochemical Engineering/Biotechnology Book Series (ABE, vol. 148).
• Perez,E., Gilbert, O., Rolland-Sabate, A., Segovia, X., Sanchez, T., reynes, M. and Dafour, D. (2011). Evaluation of the functional properties of promising Discorea trifida L. waxy starches for food innovation. Int J Carbohydrate Chem. Doi: https://doi.org/10.1155/2011/165638
• Polycarp, D., Afoakwa, E. O., Budu, A. S., and Otoo, E. (2012).Characterization of chemical composition and anti-nutritional factors in seven species within the Ghanaian yam (Dioscorea) germplasm. Int Food Res J 19 (3): 985-992 . Doi: https://doi.org/10.1007/s13197-017-2913-0
• Princewill Ogbonna I.L., Ibeji, C.C. (2015). Comparative study on nutritional and antinutritional composition of three cultivars (red, green and yellow) of aerial yam (Discorea bulbifera). IOSR J Environ Sci, Toxicol & Food Technol 9 (5):79-86. Doi: https://doi.org/10.9790/2402-09517986
• Qu X, Zhai Z, Liu X, Li H, Ouyang Z, Wu C, Liu G, Fan Q, Tang T, Qin A, Dai K.(2014). Dioscin inhibits osteoclast differentiation and bone re-sorption though down-regulating the Akt signaling cascades. Biochem Biophys Res Commun. 443(2):658–665. Doi: https://doi.org/10.1016/S1360-1385 (97)01018-2
• Rawat, M., and Parmar, N. (2013). Medicinal Plants with Antidiabetic Potential – A Review. American-Eurasian J. Agric. & Environ. Sci., 13 (1): 81-94, Doi: https://doi.org/10.5829/idosi.aejaes.2013.13.01.1890
• Rice-Evans, C.A., Miller, J., Paganga, G. (1997). Antioxidant properties of phenolic compounds. Trends Plant Sci 2:152-159.
• Sahu, P., Gidwani, B., Dhongade, H.J. (2020) Pharmacological activities of dehydroepiandrosterone: A review. Steroids.108507. Doi: https://doi.org/10.1016/j.steroids.2019.108507
• Saleha, S., Saidi, N., Muriana, S., Rasnovi, S.,Iqbalsyah, T. M. (2018). Nutritional composition of Discorea hispida from different locations around Leuser Ecosystem area. Jurnal Natural. 18 1:1-16. Doi: https://doi.org/10.24815/jn.v18i1.8504
• Sanusi, W., and Salimonu, K. (2006). Food security among households: Evidence from yam production economic in Oyo State, Nigeria. Agric J, 4: 249-253. Doi: https://doi.org/10.2298/JAS1501061Y
• Son, I.S.1., Kim, J.H., Sohn, H.Y., Son, K.H., Kim, J.S., Kwon, C.S. (2007). Anti-oxidative and hypolipidemic effects of diosgenin, a steroidal saponin of yam (Dioscorea spp.), on high-cholesterol fed rats. Biosci Biotechnol Biochem.71 (12):3063-3071. Doi: https://doi.org/10.1271/bbb.70472
• Tada, Y., Kanda, N., Haratake, Tobiishi, M., Uchiwa, H., and Wanatabe, S. (2009). Novel effects of diosgenin on skin ageing. Steroids 74 (6):504-511. Doi: https://doi.org/10.1016/j.steroids.2009.01.006 PMID:15513824. Doi: https://doi.org/10.1080/10915810490499055
• Taylor M. (1997) Alternatives to conventional hormone replacement therapy. Compr Ther. 23 (8):514-532. PMID: 928374
• Tindo J. M., Zapfack L. and Sonké B. (2015) Diversity of wild yams (Dioscorea spp., Dioscoreaceae) collected in continental Africa. Biodiversity & Conservation. 25 (1) Doi: https://doi.org/10.1007/s10531-015-1031-4
• Ukom, AN., Ojimelukwe, P.C., Ezeama, CF., Oritz, D.O., Aragon, IJ. Phenolic and antioxidant activity of some underutilized Nigerian yam (discorea spp) and cocoyam (Xanthosoma maffa Scoth) tubers. J Environ Sci, Toxicol & Food Technol 8 (7): 104-111. Doi: https://doi.org/10.9790/2402-0871104111
• Udensi, E.A., Oselebe, H.O. and Iweala, O.O. (2008). The investigation of chemical composition and functional properties of water yam (Discorea alata): Effect of varietal differences. Pak J Nutr. 7 (2): 342-344.
• Undie, S.A., Akubue, P.L. (1986). Pharmacological evaluation of Discorea dumetorum tuber used in traditional antidiabetic therapy. J. Ethnopharmacol 15:133-144. Doi: https://doi.org/10.1016/0378-8741(86)90150-9
• Verter, N., Becvarova, V. (2015). An analysis of yam production in Nigeria. Acta Universitatis Agriculturae et Silvicuturae Mendelianae Brunensis 63 (2):659-665. Doi: https://doi.org/10.11118/actaun201563020659
• Zava, D.T., Dollbaum, and C.M., Blen, M. (1998). Estrogen and progestin bioactivity of foods, herbs, and spices. Proc Soc Exp Biol Med. 217 (3):369-378. Doi: https://doi.org/10.3181/00379727-217-44247
• Wu, Z.G., Jiang, W., Nitin, M., Bao, X. Q., Chen, S.L. and Tao, Z.M. (2016). Characterizing diversity based on nutritional and bioactive compositions of yam germplasm (Discorea spp) commonly cultivated in China. J Food Drug Analysis. 24:367-375. https://doi.org/10.1016/j.jfda.2015.12.003
• Lu B, Xu Y, Xu L, Cong X, Yin L, Li H, Peng J. (2012) Mechanism investigation of dioscin against CCl4-induced acute liver damage in mice. Environ Toxicol Pharmacol. 34(2):127–135. Doi: https://doi.org/10.1016/j.etap.2012.03.010
• Stomati, M., Monteleone, P., Casarosa, E., Quirici, B., Puccetti, S., Bernardi, F., Genazzani, A.D., Rovati, L., Luisi, M., Genazzani, A. R. (2000). Six-month oral dehydroepiandrosterone supplementation in early and late post-menopause Gynecol Endocrinol. 14 (5):342-63. Doi: https://doi.org/10.3109/09513590009167703
• Sahu P, Gidwani B, Dhongade HJ. Pharmacological activities of dehydroepiandrosterone: A review. Steroids. 153:108507. Doi: https://doi.org/10.1016/j.steroids.2019.108507
• Yang, L., Ren, S., Xu, F., Ma, Z., Liu, X. and Wang, L. 2019. Recent Advances in the Pharmacological Activities of Dioscin. BioMed Research International. Doi: https://doi.org/10.1155/2019/5763602
• Zhang, Z., GaO, W., Wang, R., Huang, L. (2014). Changes in main nutrients and medicinal composition of Chinese yam (Discorea opposita) tubers during storage. J Food Sci Technol 51 (10):2535-2543. Doi: https://doi.org/10.1007/s13197-012-0776-y