Determination of Toxic Effects of Biopesticide Emamectin Benzoate in the Tissues of Oreochromis niloticus by Acetylcholinesterase Enzyme Activity
Öz
In
this study, the effects of emamectin benzoate on the AChE activity in Oreochromis niloticus were investigated.
Fishes were exposed to 110 and 220 μg / L of emamectin benzoate for 96 hours
and 14 days to determine acetylcholinesterase (AChE) activities in brain, liver
and kidney tissues.
The were
significant changes in asetilkoinesteraz
(AChE) activities in brain, liver and kidney tissues of O. niloticus exposed to emamectin
benzoate compared to controls. AChE activity in brain, liver and kidney tissues
of O. niloticus, was reduced in all periods
when compared to control values.
Anahtar Kelimeler
Emamectin Benzoate Oreochromis niloticus Acetylcholinesterase AChE toxicity
Kaynakça
- Moraes, B.S., Clasen, B., Loro, V.L., Pretto, A., Toni, C., Avila, L.A., Marchesan, E., Machado, S.L.O., Zanella, R. & Reimche, G.B. (2011). Toxicological response of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicol Environ Safe, 74, 328-335. Moraes, B.S., Loro, V.L., Pretto, A., da Fonseca, M.B., Menezes, C., Marchesan, E. & de Avila, L.A. (2009). Toxicological and metabolic parameters of the teleost fish (Leporinus obtusidens) in response to commercial herbicides containing clomazone and propanil. Pesticide biochemistry and physiology, 95(2), 57-62. Mushtaq, M., Feely, W.F., Syintsakos, L.R. & Wislocki, P.G. (1996). Immobility of emamectin benzoate in soils. J Agric Food Chem, 44, 940- 944. OECD test, (1992). NO: 203: Fish. Acute toxicity test. Rakotondravelo, M.L., Anderson, T.D., Charlton, R.E. & Zhu, K.Y. (2006). Sublethal effects of three pesticides on activities of selected target and detoxification enzymes in the aquatic midge, Chironomus tentans (Diptera: Chironomidae). Archives of environmental contamination and toxicology, 51(3), 360. Renick, V.C., Weinersmith, K., Vidal-Dorsch, D.E. & Anderson, T.W. (2016). Effects of a pesticide and a parasite on neurological, endocrine, and behavioural responses of an estuarine fish. Aquatic Toxicology, 170, 335-343. Richetti, S.K., Rosemberg, D.B., Ventura-Lima, J., Monserrat, J.M., Bogo, M.R., & Bonan, C.D. (2011). Acetylcholinesterase activity and antioxidant capacity of a zebrafish brain is altered by heavy metal exposure. Neurotoxicology, 32(1), 116-122. Roberts, T.R. & Hutson, D.H. (1999). Metabolic Pathways of Agrochemicals. The Royal Society of Chemistry, UK, 871p. Tan, J., Galligan, J.J. & Hollingworth, R.M. (2007). Agonist actions of neonicotinoids on nicotinic acetylcholine receptors expressed by cockroach neurons. Neurotoxicology, 28, 829-42. Tavakol, M. (2007). Environmental impact assessment of diazinon in rice fields (a Case Study on Amol Township Rice Fields), M.Sc. thesis, Science and Research Branch, Islamic Azad University, Tehran, Iran, 1-95pp. Wang, Y., Chen, C., Zhao, X., Wang, Q. & Qian, Y. (2015). Assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (Cyprinus carpio) using acetylcholinesterase activity as an endpoint. Pesticide biochemistry and physiology, 122, 81-85.
Biyopestisit Emamektin Benzoat’ın Oreochromis niloticus'un Dokularında Toksik Etkilerinin Asetilkolinesteraz Enzim Aktivitesiyle Belirlenmesi
Öz
Bu çalışmada emamectin benzoate’ın Oreochromis
niloticus’da AChE aktivitesi üzerine etkileri
araştırılmıştır. Balıklar 110 ve 220 µg/L emamectin benzoate etkisine 96 saat
ve 14 günlük sürelerle bırakılarak beyin, karaciğer ve böbrek dokularında
asetilkoinesteraz (AChE) aktiviteleri belirlenmiştir.
Emamectin benzoate maruz bırakılan O. niloticus'un beyin, karaciğer ve
böbrek dokularında asetilkoinesteraz (AChE) enzim aktivitesi kontrol
değerleriyle ile karşılaştırıldığında önemli değişiklikler belirlenmiştir Emamectin benzoate’a maruz bırakılan O.
niloticus'un beyin, karaciğer ve böbrek dokularında AChE aktivitesi kontrol
değerleriyle ile karşılaştırıldığında tüm sürelerde azaldığı belirlenmiştir.
Anahtar Kelimeler
Emamectin Benzoate Oreochromis niloticus Asetilkoinesteraz AChE toksisite
Kaynakça
- Moraes, B.S., Clasen, B., Loro, V.L., Pretto, A., Toni, C., Avila, L.A., Marchesan, E., Machado, S.L.O., Zanella, R. & Reimche, G.B. (2011). Toxicological response of Cyprinus carpio after exposure to a commercial herbicide containing imazethapyr and imazapic. Ecotoxicol Environ Safe, 74, 328-335. Moraes, B.S., Loro, V.L., Pretto, A., da Fonseca, M.B., Menezes, C., Marchesan, E. & de Avila, L.A. (2009). Toxicological and metabolic parameters of the teleost fish (Leporinus obtusidens) in response to commercial herbicides containing clomazone and propanil. Pesticide biochemistry and physiology, 95(2), 57-62. Mushtaq, M., Feely, W.F., Syintsakos, L.R. & Wislocki, P.G. (1996). Immobility of emamectin benzoate in soils. J Agric Food Chem, 44, 940- 944. OECD test, (1992). NO: 203: Fish. Acute toxicity test. Rakotondravelo, M.L., Anderson, T.D., Charlton, R.E. & Zhu, K.Y. (2006). Sublethal effects of three pesticides on activities of selected target and detoxification enzymes in the aquatic midge, Chironomus tentans (Diptera: Chironomidae). Archives of environmental contamination and toxicology, 51(3), 360. Renick, V.C., Weinersmith, K., Vidal-Dorsch, D.E. & Anderson, T.W. (2016). Effects of a pesticide and a parasite on neurological, endocrine, and behavioural responses of an estuarine fish. Aquatic Toxicology, 170, 335-343. Richetti, S.K., Rosemberg, D.B., Ventura-Lima, J., Monserrat, J.M., Bogo, M.R., & Bonan, C.D. (2011). Acetylcholinesterase activity and antioxidant capacity of a zebrafish brain is altered by heavy metal exposure. Neurotoxicology, 32(1), 116-122. Roberts, T.R. & Hutson, D.H. (1999). Metabolic Pathways of Agrochemicals. The Royal Society of Chemistry, UK, 871p. Tan, J., Galligan, J.J. & Hollingworth, R.M. (2007). Agonist actions of neonicotinoids on nicotinic acetylcholine receptors expressed by cockroach neurons. Neurotoxicology, 28, 829-42. Tavakol, M. (2007). Environmental impact assessment of diazinon in rice fields (a Case Study on Amol Township Rice Fields), M.Sc. thesis, Science and Research Branch, Islamic Azad University, Tehran, Iran, 1-95pp. Wang, Y., Chen, C., Zhao, X., Wang, Q. & Qian, Y. (2015). Assessing joint toxicity of four organophosphate and carbamate insecticides in common carp (Cyprinus carpio) using acetylcholinesterase activity as an endpoint. Pesticide biochemistry and physiology, 122, 81-85.
Ayrıntılar
| Birincil Dil | Türkçe |
|---|---|
| Bölüm | Makaleler |
| Yazarlar | |
| Yayımlanma Tarihi | 30 Nisan 2019 |
| Gönderilme Tarihi | 19 Şubat 2019 |
| Kabul Tarihi | 29 Mart 2019 |
| Yayımlandığı Sayı | Yıl 2019 Cilt: 4 Sayı: 1 |
