Carduus adpressus’un Çeşitli Ekstrelerinin Antioksidan Özellikleri ve Antimikrobiyal Aktivitesi Üzerine Bir Çalışma

Yıl 2019, Cilt: 4 Sayı: 3, 409 - 413, 30.12.2019

Tuba Acet

https://doi.org/10.35229/jaes.619647

Öz

Carduus
cinsi, Anadolu halk tıbbında pek çok rahatsızlığın giderilmesinde geleneksel
olarak kullanılır. Bu çalışmada, Carduus
adpressus’un farklı bitki ekstrelerinin potansiyel antioksidan özellikleri
ve antimikrobiyal aktivitesi incelendi. Toplam polifenoller, toplam fenolik ve
toplam flavonoid içerikleri sırasıyla fosfomolibdat, Folin-Chiocalteu reaktifi
ve AlCl₃ ile yapıldı. Antioksidan aktivite, ABTS (2,2´-azino-bis,
3-etilbenzotiazolin-6-sülfonik asit) ve DPPH (2,2-difenil-1-pikrilhidrazil)
metodları kullanılarak tespit edildi. Antimikrobiyal aktivite ise, disk
difüzyon ve mikrodilüsyon yöntemleri kullanılarak ortaya çıkarıldı. Etanol
ekstresi en yüksek toplam antioksidan (377.9 mg GAE g-1 ekstre),  flavonoid (60,06 mg QE g^-1 ekstre)
ve fenolik (291,03 mg GAE g^-1 ekstre) içeriğe sahipti. Ayrıca, en
güçlü radikal temizleme etkinliği, etanol ekstresinde (ABTS: 78,26 mg TE g^-1
ekstre ve DPPH: 74,45 mg TE g^-1 ekstre) tespit edildi. Tüm ekstreler
en az bir mikroorganizmaya karşı yüksek bir antimikrobiyal aktivite sergiledi.
Sonuç olarak, C. adpressus bitkisinin
doğal bir antioksidan ve antimikrobiyal ajan olarak kullanılma potansiyeli
olduğu düşünülmektedir.

Anahtar Kelimeler

Antioksidan özellik, antimikrobiyal aktivite, Carduus adpressus

A Study on Antioxidant Properties and Antimicrobial Activity of Various Extracts of Carduus adpressus

Öz

The
genus Carduus is traditionally used to relieve many ailments in Anatolian folk
medicine. In this study, potential antioxidant properties and antimicrobial
activity of different plant extracts of Carduus adpressus were investigated.
Total polyphenols, total phenolic and total flavonoid contents were made with
phosphomolybdate, Folin-Chiocalteu reagent and AlCl₃, respectively.
Antioxidant activity was determined using ABTS (2,2´-azino-bis,
3-ethylbenzothiazoline-6-sulfonic acid) and DPPH
(2,2-diphenyl-1-picrilhydrazil) methods. Antimicrobial activity was determined
by disc diffusion and microdilution methods. Ethanol extract had the highest
total antioxidant content (377.9 mg GAE g^-1 extract), flavonoid
(60.06 mg QE g^-1 extract) and phenolic (291.03 mg GAE g^-1
extract). In addition, the strongest radical scavenging activity was detected
in ethanol extract (ABTS: 78.26 mg TE g^-1 extract and DPPH: 74.45 mg
TE g^-1 extract). All extracts exhibited a high antimicrobial
activity against at least one microorganism. As a result, C. adpressus plant is thought to have potential to be used as a
natural antioxidant and antimicrobial agent.

Anahtar Kelimeler

Antioxidant property, antimicrobial activity, Carduus adpressus

Kaynakça

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