Preparation of O-antigen from Yersinia ruckeri Serotype O1 and Use in the Slide Agglutination Test

Yıl 2019, Cilt: 4 Sayı: 3, 480 - 483, 30.12.2019

Fikri Balta , Zeynep Dengiz Balta

https://doi.org/10.35229/jaes.645416

Cited By: 3

Öz

In this study, it was aimed at early detection of yersiniosis caused by Yersinia ruckeri in rainbow trouts (Oncorhynchus mykiss) using agglutination
test. O-antigen of Y. ruckeri
isolated from diseased fishes was prepared. This prepared O-antigen was
injected into the ear vein of the rabbit. Serum was removed from blood samples
taken on 7th and 15th days after the last injection. Thus, the antibody of the Yersinia ruckeri was produced in the
rabbit. Titers of the resulting antibodies were determined by microtitering in
96 well-based plates. It was used successfully in the slide agglutination tests
by making necessary dilutions according to the titer ratios of the antibodies. We
determined that the motile and Gr (-) bacteria isolated from diseased rainbow
trout with yersiniozis symptom were Y.
ruckeri according to the results of slide agglutination test without any
biochemical test. The isolated bacteria were confirmed to be Y. ruckeri with the results of API 20E
test kit and PCR test. The antibodies were stored at -20 and -80°C for use
subsequent studies.

Anahtar Kelimeler

Yersinia ruckeri, O antigen, slide agglutination test

Kaynakça

• Austin, B. & Austin, D. (1987). Bacterial fish pathogens. Diseases in famed and wild fish. First published, Ellis Horwood Ltd., Chichester, UK, 364 p.
• Austin, B. & Austin, D. (1999). Bacterial fish pathogens. Diseases of farmed and wild fish, 3rd edn., Praxis Publishing Ltd, Chichester, UK, 457p.
• Austin, B. & Austin, D. (2007). Bacterial fish pathogens. Diseases of farmed and wild fish, 4th edn. Praxis Publishing, Chichester.
• Altun, S., Onuk, E.E., Çiftci, A., Duman M. & Büyükekiz, A.G. (2013). Determination of Phenotypic, Serotypic and Genetic Diversity and Antibiotyping of Yersinia ruckeri Isolated from Rainbow Trout. Kafkas Üniversitesi Veteriner Fakültesi Dergisi, 19(2), 225-232. Doi: 10.9775/kvfd.2012.7606.
• Balta, F., Çağırgan, H. & Kayış, Ş. (2005). Kültürü yapılan gökkuşağı alabalığında (Oncorhynchus mykiss) izole edilen Yersinia ruckeri'nin identifikasyonunda API 20E testinin kullanılabilirliği. Türk Sucul Yaşam Dergisi, 3(4), 434-437.
• Balta, F., Sandalli, C., Kayis, S. & Ozgumus, O.B. (2010). Molucular analysis of antimicrobial resistance in Yersinia ruckeri strains isolated from rainbow trout (Onchorhynchus mykiss) grown in commercial fish farms in Turkey. Bulletin of the European Association of Fish Pathologists, 30(6) 211-219.
• Balta, F., Dengiz Balta, Z., Özgümüş O.B. & Çağırgan H. (2016). Doğu Karadeniz Bölgesi’ndeki Gökkuşağı Alabalığı (Oncorhynchus mykiss) Çiftliklerinde Yersinia ruckeri’nin Portörlük Yönünden Tetkiki ve Antimikrobiyal Direncin Tespiti. Journal of Anatolian Environmental & Animal Sciences, 1(3), 72-76.
• Çağırgan, H. (1995). Ege bölgesinde görülen Yersiniosis vakaları ve tedavisi, Atatürk Üniversitesi Ziraat Fakültesi, Doğu Anadolu Bölgesi, II. Su Ürünleri Sempozyumu, Erzurum, 316-329.
• Çağırgan, H. & Yüreklitürk, O. (1991). First isolation of Yesinia ruckeri from Rainbow trout farm in Turkey. In: The Fifth Coference of EAFP, Disease of Fish and shellfish. 24-29 August 1991, Book of Abstract, p. 131.
• Çağirgan, H. & Tanrikul, T.T. (1998). Testing the effectiveness of a yersinia vaccine in infected and chemically treated juvenile rainbow trout (Onchorhynchus mykiss). Journal of Applied Ichthyology, 14, 239-243.
• Davies, R.L. (1990). O-serotyping of Yersinia ruckeri with special emphasis on European isolates. Veterinary Microbiology, 22, 299-307. Doi:10.1016/0378-1135(90)90016-O.
• Diler, Ö., Demirkan, T., Altun, S. & Tural, F. (1998). Fethiye bölgesindeki bazı alabalık işletmelerinde Yersiniosis’in mevsimsel dağılımı üzerine bir araştırma, Atatürk Üniversitesi Ziraat Fakültesi, III. Su Ürünleri Sempozyumu, Erzurum, 207-220.
• Ewing, W.H. (1972). Production af antisera, Pages 198-205 in P.R. Edwards and W.H. Ewing, editörs. Identification of Enterobacteriaceae, 3rd ed. Burgess Publishing company, Mineapolis, Minesota.
• Furones, M.D., Rodgers, C.J. & Munn, C.B. (1993). Yersinia ruckeri, the causal agent of enteric redmouth disease (ERM) in fish. Annual Review of Fish Diseases, 3, 105-125.
• Onuk, E.E., Ciftci, A., Findik, A., Ciftci, G., Altun, S., Balta, F., Ozer, S. & Coban, A.Y. (2011). Phenotypic and molecular characterization of Yersinia ruckeri isolates from Rainbow Trout (Oncorhynchus mykiss, Walbaum, 1792) in Turkey, Berliner und Munchener Tierarztliche Wochenschrift, 124(7-8), 320-328.
• Ormsby, M.J., Caws, T., Burchmore, R., Wallis, T., Verner-Jeffreys, D.W. & Davie, R.L. (2016). Yersinia ruckeri isolates recovered from diseased Atlantic salmon (Salmo salar) in Scotland are more diverse than those from rainbow trout (Oncorhynchus mykiss) and represent distinct subpopulations. Applied and Environmental Microbiology, 82(19), 5785-5794.
• Waltman, W.D. & Shotts E.B. (1984). A medium for the isolation and differentiation of Yersinia ruckeri. Canadian Journal of Fisheries and Aquatic Sciences, 41, 804-808.