Gökkuşağı Alabalığı Bağırsaklarından İzole Edilen Laktik Asit Bakterilerinin Lactococcus garvieae’ye Karşı Probiyotik Potansiyelinin İn Vitro Olarak Belirlenmesi

Yıl 2020, Cilt: 5 Sayı: 4, 647 - 654, 31.12.2020

Dilek Pehlivan Ertan Emek Onuk

https://doi.org/10.35229/jaes.821270

Öz

Bu çalışma amacı, gökkuşağı alabalıklarının bağırsak mikroflorasından Lactococcus garvieae’ye karşı antagonostik etkiye sahip Laktik Asit Bakterilerinin (LAB) izole edilmesi ve antagonostik etkiye sahip olduğu belirlenen bakteriyel türlerin olası probiyotik potansiyelinin in vitro olarak ortaya konulmasıdır. Bu amaçla Gökkuşağı alabalıklarının bağırsak florasından LAB türleri konvansiyonel kültür metotları kullanılarak izole edildi. Elde edilen izolatların L. garvieae’ye karşı antagonostik etkinlikleri agar well difüzyon testi ile belirlendi. Sonraki aşamada antagonostik aktiviteye sahip olduğu belirlenen izolatların hidrofobisitesi, pH ve safra tolerans düzeyleri ve antibiyotik duyarlılık profilleri belirlendi. Elde edilen aday probiyotik bakterilerinin genetik identifikasyonu PCR metodu ile gerçekleştirildi. Balıkların bağırsak florasından toplamda 47 izolat elde edildi. Bu izolatların 6’sının L. garvieae’ye karşı antagonostik etkinliğe sahip olduğu belirlendi. İleri testlerde izolatların hidrofobik özellikte oldukları, yüksek safra ve düşük pH şartlarına dirençli oldukları ve izolatların tamamının beş farklı antibiyotiğe karşı dirençli olduğu belirlendi. Genetik identifikasyon sonucu izolatlardan beşi Lactobacillus acidophilus olarak identifiye edildi. Bir izolat ise Lactobacillus spp. olarak tanımlandı. Bu çalışma ile ülkemiz kültür balığı yetiştiriciliğinde yaygın olarak görülen L. garvieae’nın kontrol altına alınmasında kullanılabilecek olası probiyotik bakteriler elde edilmiştir.

Anahtar Kelimeler

Gökkuşağı alabalığı, Laktik asit bakterileri, Lactococcus garvieae, Probiyotik

In Vitro Evaluation of Probiotic Potential of Lactic Acid Bacteria Isolated from Rainbow Trout Gut against Lactococcus garvieae

Öz

The purpose of this study are to isolate Lactic Acid Bacteria (LAB), which have an antagonistic activity against Lactococcus garvieae, in the intestinal microflora of rainbow trouts and to reveal in vitro, the probable probiotic potential of the bacterial species, which were determined to have an antagonistic effect. For this aim LAB species were isolated from the intestinal flora of rainbow trouts using conventional culture methods. The antagonistic efficiencies of the obtained isolates against L. garvieae were determined with the agar well diffusion test. The hydrophobicity, pH and bile tolerance levels and antibiotic susceptibility profiles of the isolates, which were found to have antagonistic activity, were determined in the next stage. Genetic identification of the obtained candidate probiotic bacteria was performed with PCR method. A total of 47 isolates were obtained from the intestinal flora of the rainbow trout. Six of these isolates were determined to have antagonistic activity against L. garvieae. Further tests revealed that the isolates were hydrophobic, resistant to high bile and low pH conditions, and that all isolates were resistant to five different antibiotics. Following genetic identification, five of the isolates were identified as Lactobacillus acidophilus. One of the isolates was defined as Lactobacillus spp. With this study, possible probiotic bacteria were obtained, which can be used to control L. garvieae, widespread in our country's culture fish farming.

Anahtar Kelimeler

Lactic acid bacteria, Lactococcus garvieae, Probiotic, Rainbow trout

Kaynakça

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