Manda Mastitis Kökenli Streptococcus agalactiae İzolatlarının Virülens Belirleyicileri

Yıl 2024, Cilt: 9 Sayı: 2, 196 - 204, 30.06.2024

Şeyda Yaman , Murat Karahan , Alper Çiftci , Arzu Findik

https://doi.org/10.35229/jaes.1454258

Öz

Mastitisin bir nedeni olan streptokoklar, büyük ekonomik kayıplar nedeniyle süt endüstrisi için dünya çapında önemli bir sorundur. Streptococcus agalactiae, bulaşıcı mastitis patojeni olarak, süt sığırlarında ve mandalarda mastitisin önemli bir nedeni olmaya devam etmektedir. Bu çalışma manda sütünden izole edilen S. agalactiae suşlarının virulens belirleyicilerini belirlemek amacıyla gerçekleştirilmiştir.
Çalışma kapsamında manda mastitisinden elde edilen 24 adet S. agalactiae izolatı incelendi. İzolatların biyofilm üretimi fenotipik olarak CRA yöntemiyle araştırıldı. İzolatların 18'inin (%75) biyofilm üretimi açısından pozitif olduğu görüldü. S. agalactiae izolatlarında hylB, fnbB, scpB ve spb1 virülens genlerinin varlığı PCR ile belirlendi. İzolatların 19'unun (%79,17) scpB, 6'sının (%25) fnbB geni yönünden pozitif olduğu görüldü. İzolatların hiçbirinde hylB ve spb1 virülens genleri belirlenemedi. Kanamisin, ampisilin, enrofloksasin, eritromisin, tetrasiklin, trimetoprim-sülfametoksazol antibiyotik diskleri ile izolatların antibiyotik direnç profilleri Kirby Bauer Disk Difüzyon Yöntemi ile belirlendi ve direnç oranları sırasıyla %41,7, %45,9, %25, %12,5, %20,9 ve %33,3 olarak değerlendirildi. Tüm izolatların RAPD-PCR paternleri ERIC-2 primeri kullanılarak belirlendi ve RAPD paternlerinin dendrogramları UPGMA yöntemiyle çizildi. İzolatların %59-95 arasında benzerlik gösterdiği belirlendi.
Sonuç olarak; bu araştırma ile manda mastitisinden izole edilen S. agalactiae izolatarında çeşitli virülens genlerinin prevalansı belirlendi. Mandalardan izole edilen S. agalactiae'nin moleküler epidemiyolojisi ve değişkenliğinin belirlenmesi ve S. agalactiae ile ilgili mastitis kontrol programlarının geliştirilmesi amacıyla daha ileri çalışmalara ihtiyaç olduğu kanısına varıldı.

Anahtar Kelimeler

fnbB, hylB, manda, S. agalactiae, scpB, spb1, virulens belirleyicileri

Virulence Determinants of Buffalo Mastitis Originated Streptococcus agalactiae Isolates

Öz

Streptococci as a cause of mastitis have become the major concern to the dairy industry worldwide due to huge economic losses. Streptococcus agalactiae is a major contagious mastitis pathogen and continues to be a major cause of mastitis in dairy cattle and buffaloes. The aim of the study was to investigate the virulence determinants of S. agalactiae strains isolated from buffalo milk.
Within the scope of the study, 24 S. agalactiae isolates from buffalo mastitis were examined. Biofilm production of isolates was investigated phenotypically by CRA method. It was observed that 18 (75%) isolates were positive for biofilm production. The presence of hylB, fnbB, scpB and spb1 virulence genes in S. agalactiae isolates were investigated by PCR. It was determined that 19 (79.17%) of the isolates were positive for scpB and 6 (25%) for fnbB virulence genes. None of the isolates were found to contain hylB and spb1 virulence genes. The antibiotic resistance profiles of the isolates among kanamycin, ampicillin, enrofloxacin, erythromycin, tetracycline, trimethoprim-sulfamethoxazole antibiotic discs were determined by Kirby Bauer Disc Diffusion Method. Resistance were evaluated as % 41.7, % 45.9, % 25, % 12.5, % 20.9, and % 33.3, respectively. RAPD-PCR patterns of all isolates were determined using the ERIC-2 primer. The dendrograms of the RAPD patterns were plotted with the UPGMA method. It was determined that the isolates showed similarity between 59-95%.
In conclusion, the research confirms the prevalence of various virulence genes in S. agalactiae isolated from buffalo mastitis. Further studies are therefore necessary to determine the molecular epidemiology and variability of S. agalactiae isolated from buffaloes, with the aim of improving mastitis control programs with regard to S. agalactiae.

Anahtar Kelimeler

buffalo, fnbB, hylB, S. agalactiae, scpB, spb1, virulence genes

Kaynakça

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