Inhibition of Toxic Effects of Oxalate in Sugar Beet Pulp with Adding Ca During Ensiling

Yıl 2021, Cilt: 4 Sayı: 2, 164 - 173, 27.12.2021

Cüneyt Temür , Mehtap Güney , Sibel Erdoğan , Murat Demirel

https://doi.org/10.51970/jasp.1012891

Öz

This study was carried out to eliminate the toxic effect of soluble oxalate in sugar beet pulp (SBP) by ensiling and adding Ca during ensiling and to determine the effects of the obtained silage on sheep. The study was based on two experiments. For the first experiment, the laboratory silages were prepared as SBP alone with the addition of molasses and straw at different levels of dry matter and Ca. After fermentation for 120 days, the Fleig scores, physical properties, Weende analysis, and oxalate analysis were performed. This laboratory trial showed that the content of the soluble oxalate could be reduced to nontoxic levels by ensiling and adding Ca. For the second experiment, SBP silage with 30% dry matter (DM) and 45 mg/kg SBP were prepared according to the results of the first experiment. This silage was fed to 36 sheep divided into six groups. During the experiment, blood samples and rumen fluid was collected every 14 days during the experiment, and the body weights were controlled. The results of this experiment showed that the soluble oxalate amount of SBP decreased to a tolerable level by the rumen. Thus, ensiling SBP with barley straw, molasses, urea, and di-calcium phosphate (DCP), as a calcium source, showed that soluble oxalate levels of SBP could be reduced levels below the toxic levels, and added to sheep ratio as 60%.

Anahtar Kelimeler

oxalate, toxic, şeker pancarı posası, silage

Destekleyen Kurum

YYÜ BAP

Proje Numarası

376-2006-ZF-B01

Şeker Pancarı Posasında Bulunan Oksalatın Toksik Etkisinin Silolama ve Ca ilavesi ile Giderilmesi

Öz

Bu çalışma, şeker pancarı posasının (ŞPP) silolama ve silolama sırasında kalsiyum (Ca) ilavesinin çözünebilir oksalat üzerine etkilerini belirlemek ve bu silajın koyunlar üzerindeki etkilerini belirlemek amacıyla yapılmıştır. Çalışma iki deneme halinde yürütülmüştür. İlk deneme tek başına ŞPP silajı ile farklı kuru madde ve Ca seviyelerinde üre, melas ve saman ilavesi ile laboratuvar silajları olarak hazırlanmıştır. 120 günlük fermentasyondan sonra Fleig puanları, fiziksel özellikler, Weende analizleri ve oksalat analizleri yapılmıştır. Bu laboratuvar denemesi, silolama ve Ca ilavesinin çözünür oksalat miktarının, ruminantlar için toksik olmayan seviyelere düşürülebileceğini göstermiştir. İkinci deneme için birinci denemenin sonuçlarına göre %30 kuru madde (DM) ve 45 mg/kg ŞPP Ca içeren ŞPP silajı hazırlanmıştır. Bu silaj altı gruba ayrılan 36 koyuna yedirilmiştir. Deneme süresince 14 günde bir kan örnekleri ve rumen sıvısı alınmış, vücut ağırlıkları kontrol edilmiştir. Yapılan iki denemenin sonuçları, ŞPP'nin çözünür oksalat miktarının rumen tarafından tolere edilebilir bir düzeye düşürülebileceğini göstermiştir. Bu nedenle, ŞPP’na arpa samanı, melas, üre ve kalsiyum kaynağı olarak dikalsiyum fosfat (DCP) ilavesi ile silajlanarak çözünür oksalat düzeylerinin toksik seviyelerin altına düşürülebileceği ve koyun rasyonlarına %60 oranında katılabileceği kanaatine varılmıştır.

Anahtar Kelimeler

okzalat, toksik, şeker pancarı posası, silaj

Proje Numarası

376-2006-ZF-B01

Kaynakça

• AOAC, 1990. Association of official analytical chemistry. Official Methods of Analysis,15th,Vol:1, Washington,D.C.
• Alçiçek, A. and Özkan, K. 1997. Determination of silage quality with physical and chemical methods in silages. Turkey I. Silage Congress, 16- 19 September Bursa. 241-246.
• Aslani, M.R., Movassaghi, A.R., Najarnezhad, V., Pirouz, H.J. and Bami, M.H. 2011. Acute oxalate intoxication associated to ingestion of eshnan (Seidlitzia rosmarinus) in sheep. Trop Anim Health Prod:43:1065–1068 DOI 10.1007/s11250-011-9818-0.
• Azcarate-Peril, M.A.; Bruno-Ba´rcena, J.M.; Hassan, H.M. and Klaenhammer, T.R. 2006. Transcriptional and functional analysis of oxalyl-coenzyme A (CoA) decarboxylase and formyl-CoA transferase genes from Lactobacillus acidophilus. Applied and Environmental Microbiology. 1891–1899.
• Balikci, E. and Gürdogan, F. 2002. The effect of wet sugar beet pulp fed as the only forage source on some heamatologic and biochemicial parameters in lambs. YYU Veterinary Faculty Journal, 13 (1-2):50-53.
• Brogren, M. and Savage, G.P. 2003. Bioavailability of soluble oxalate from spinach eaten with and without milk products. Asia Pacific Journal of Clinical Nutrition, 12 (2): 219-224.
• Bulgurlu, S. and Ergul, M. 1978. Analyses methods of physical, chemical and biological of feeds. Ege University Press, Issue Number: 127,176p
• Gündüz, T. 1993. The Quantitative Analysis Laboratory Book. Bilge Publications, Ankara.
• Hart, S.P. and Horn, F.P. 1987. Ensiling characteristics and digestibility of combinations of turnips and wheat straw. Journal of Animal Science, 64:1790-1800.
• James, L.F., Street, J.C.and Butcher, J.E. 1972. Halogeton poisoning of sheep: Effect of high level oxalate intake. Journal of Animal Science,35 (6): 1233-8
• James, L.F., Street, J.C., Butcher, J.E. and Shupe, J.L. 1968. Intake on electrolyte metabolism Glomeratus halogeton. Oxalate Metabolism in Sheep II. Effect of Low Level. Journal of Animal Science, 27:724-729.
• Kaya, S. and Pirincci, L. 1995. Veterinary Clinical Toxicology. Glycosides. Medisan Publications, Ankara.
• Kilic, A. 1986. Silage . Bilgehan Printing House. İzmir, Turkey 350 p.
• Mc Kenzie, R.A., Bell, A.M., Storie, G.J., Kenan, F.J., Cornack, K.M. and Grant, S.G. 1988. Acute oxalate poisoning of sheep by buffelgrass (Cenchrus ciliaris). Australian Veterinary Jornal, 65: 26.
• Miyazaki, S., Yamanake, N. and Guruge,.K. 2003. Simple capillary electrophoretic determination of soluble oxalate and nitrate in forages grasses. Journal of Veterinary Diagnostic Investigation, 15:480-483.
• Muck, R.E. and Dickerson, J.T. 1988. Storage temperature effects on proteolysis in alfalfa silage. Transactions of the American Society of Agriculture Engineering, 31:1005–1009.
• Rahman M.M., Abdullah, R.B. and Wan Khadijah, W.E. 2013. A review of oxalate poisoning in domestic animals: tolerance and performance aspects. Journal of Animal Physiology and Animal Nutrition, 97:605-614.
• Rahman, M. M., Nakagawa, T., Niimi, M., Fukuyama, K. and Kawamura, O. 2011. Effects of Feeding Oxalate Containing Grass on Intake and the Concentrations of Some Minerals and Parathyroid Hormone in Blood of Sheep. Asian-Aust. J. Anim. Sci. Vol. 24, No. 7 : 940 - 945
• S.A.S., 2007. SAS® User’s Guide: Statistics, Version 8. SAS Institute Inc., Cary, NC.
• Sharma, S., Vaidyanathan, S., Nath, R. and Thind, S.K. 1991. Advances in pathophysiology of calcium oxalate stone disease. Indian Journal of Urology, 8: 25-37.
• Svedruzic, D., Jo´nssona, S., Toyota, C.G., Reinhardtb, L.A., Ricagnoc, S., Lindqvistb, Y. and Richards, N.G.J. 2005. The enzymes of oxalate metabolism: unexpected structures and mechanisms. Archives of Biochemistry and Biophysics. 433:176–192.