EBER In Situ Hybridization Experience In Hodgkin Lymphoma

Year 2021, Volume: 5 Issue: 3, 201 - 204, 20.09.2021

Gulen Gul , Mehmet Ali Özcan , Sermin Özkal

https://doi.org/10.30621/jbachs.985629

Cited By: 1

Abstract

Purpose
Several studies suggest that the relation of Epstein-Barr virus (EBV) and Hodgkin lymphoma (HL). EBV and HL associations are reported at rates ranging from 20% to 100% that varies nationally and regionally. In this study; in the case of Hodgkin's lymphoma, the presence of EBV encoded RNA (EBER) by in situ hybridization method and it’s distribution according to histopathological subtypes were investigated.
Methods
We investigated the presence of EBV in Hodgkin Lymphoma diagnosed 107 adult cases between 2013 and 2017, which chromogenic in situ hybridization with EBV RNA probe has been in routine use were evaluated retrospectively with pathology reports.
Results
There were 69 males and 38. Nodular Sclerosis Classical Hodgkin Lymphoma (NSCHL) was the most common (63 cases, 58,9%) followed by Mixed Cellularity Classical Hodgkin Lymphoma (MCCHL) (23 cases,21,5%). EBV was expressed in 40 cases (45,4%). High EBV association was found with NSCHL (50%) and MCCHL (48%). None of NLPHL was positive with EBER.
Conclusion
In our series NSCHL is the most common histologic subtype and has higher percentages of EBV positivity as reported in developed countries. Our study has the largest series of patients investigating the relationship between HL and EBER in our country. For further comments more studies, particularly from Turkey, are needed to determine the actual epidemiological pattern of HL in the region and to define the role of EBV in HL.

Keywords

EBER, EBV, Hodgkin Lymphoma

References

• 1. Stein H, Delsol G, Pileri SA, Weiss L.M, Poppema S, Jaffe E. Classical Hodgkin lymphoma, pathology and genetics. In: Swerdlow S, Campo E, Harris N, Jaffe E, Pileri S, Stein H, Thiele J, Vardiman J, editors. WHO Classification of Tumours Pathology & Genetics: Tu-mours of haematopoietic and lymphoid tissues. Lyon, France: IARC Press, 2008: 326-329
• 2. Glaser SL, Lin RJ, Stewart SL, et al. Epstein-Barr virus-associated Hodgkin's disease: epi-demiologic characteristics in international data. Int J Cancer. 1997 Feb 7;70(4):375-82
• 3. Makar RR, Saji T, Junaid TA. Epstein-Barr virus expression in Hodgkin's lymphoma in Ku-wait. Pathol Oncol Res. 2003;9(3):159-65
• 4. Axdorph U, Porwit-MacDonald A, Sjoberg J, et al. Epstein-Barr virus expression in Hodgkin's disease in relation to patient characteristics, serum factors and blood lymphocyte function. Br J Cancer. 1999;81: 1182-1187. Available from: https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2374328/pdf/81-6690827a.pdf
• 5. Weinreb M, Day PJ, Niggli F, et al. The role of Epstein-Barr virus in Hodgkin's disease from different geographical areas. Arch Dis Child. 1996 Jan;74(1):27-31
• 6. Herbst H, Steinbrecher E, Niedobitek G, et al. Distribution and phenotype of Epstein-Barr virus-harboring cells in Hodgkin's disease. Blood. 1992 Jul 15;80(2):484-91
• 7. Pallesen G, Sandvej K, Hamilton-Dutoit SJ, Rowe M, Young LS. Activation of Epstein-Barr virus replication in Hodgkin and Reed-Sternberg cells. Blood. 1991 Sep 1;78(5):1162-1165.
• 8. Flavell KJ, Murray PG. Hodgkin’s disease and the Epstein-Barr virus. Molecular Pathology. 2000;53(5):262-269
• 9. Zeytinoğlu A, Hekimgil M, Erensoy S, et al. Lenfomalı hastaların dokularında Epstein-Barr Virus DNA ve RNA’sının araştırılması. MİKROBİYOL BÜLT 2005; 39: 473-481 Available from: http://mikrobiyolbul.org/managete/fu_folder/2005-04/2005-39-04-473-481.pdf
• 10. Sadal Benzer E, Demir S, Bilir G, Akçay EY, Arabacı E. Klasik Hodgkin lenfomalarda EBV-LMP 1 ekspresyonunun histopatolojik subtiplere göre dağılımı. Ege Tıp Dergisi 2006; 1 ( 45 ) : 1 – 4 Available from: http://egetipdergisi.com.tr/pdf/pdf_EGE_229.pdf
• 11. Özdil A, Doğanay L, Demir M, Öz Puyan F, Bilgi S. Detection of Epstein Barr Virus in Hodgkin’s Disease in Trakya Region of Turkey; by in Situ Hybridization. Turk J Hematol. 2002; 19(4): 461-464. 12. Kaya H, Erman Z, Gündoğdu C, Tekin SB, Gündoğdu M. Epstein-Barr Virus in Hodgkin’s Disease Patients in Northeast Anatolia. Turk J Hematol. 2000; 17(2): 61-65
• 13. Herling M, Rassidakis GZ, Medeiros LJ, et al. Expression of Epstein-Barr virus latent membrane protein-1 in Hodgkin and Reed-Sternberg cells of classical Hodgkin's lymphoma: associations with presenting features, serum interleukin 10 levels, and clinical outcome. Clin Cancer Res. 2003 Jun;9(6):2114-20.
• 14. Glavina-Durdov M, Jakic-Razumovic J, Capkun V, Murray P. Assessment of the prognostic impact of the Epstein–Barr virus-encoded latent membrane protein-1 expression in Hodgkin’s disease. British Journal of Cancer. 2001;84(9):1227-1234.
• 15. Oudejans JJ, Jiwa NM, Meijer CJ. Epstein-Barr virus in Hodgkin's disease: more than just an innocent bystander. J Pathol. 1997 Apr;181(4):353-6.
• 16. Yilmaz F, Uzunlar AK, Sogutcu N, Ozaydin M. Hodgkin's disease and association with Epstein-Barr virus in children in Southeast Turkey. Saudi Med J. 2005 Apr;26(4):571-5