Effects of Glucosinolates and their Hydrolysis Products on Energy Balances and Performance Parameters and Histological Parameters in Broiler Chicken Diets

Year 2018, Volume: 12 Issue: 35, 31 - 41, 29.09.2018

Deniz Belenli Ümit Polat , Hatice Erdost , Tuncay İlhan , Abdülkadir Orman , Derya Yeşilbağ , Mark A. Berhow

Abstract

Glucosinolates are important bioactive molecules and widely found in Brassicaceae species (cress, brussels sprouts, mustard, broccoli, kale etc.). Depending on the amount of these vegetables consumed, both positive and negative metabolic effects from glucosinolate metabolites may occur. The aims of this study were to; investigate inexpensive animal food sources that both increases weight gain and provides enhanced performance parameters without adversely affecting the animal’s health and metabolism; to evaluate dose adjustment of food containing glucosinolates in animals; and to evaluate changes in the biochemical and performance status of chickens on these glucosinolate containing diets. A total of 624 one-day-old Ross 308 broiler line chicks were divided into one control and three treatment groups. Cress seed (Lepidium sativum) was added 0.05% for the first treatment groups (Group 1, 10 g/kg), 0.10% for the second treatment groups (Group 2, 20 g/kg) and 0.15% for the last treatment groups (Group 3, 30 g/kg) to the diet. Serum samples were evaluated for serum glucose, adiponectin, leptin, growth hormone, estradiol and cortisol levels. Performance parameters investigated included feed intake, live body weight and feed conversion ratio. . The villus length, number of goblet cells, crypt depth were determined for histological analyses. According to histological results, villus length was significant at p < 0.05 level between control and group 1 and at p < 0.001 level with groups 2 and 3 at 21 days. The depth of the crypts belonging to the control and experimental groups was not significant between the control group and the group 1 when the statistic was evaluated on the 21st day, whereas between the control group and the group 2, p < 0.05 and p < 0.01; statistical significance was found at p < 0.001 level between group 3 and control group. The results showed that dietary glucosinolate supplementation as feed additive (10, 20 and 30 g/kg) did not significantly improved the dietary performance, or carcass parameters of broiler chickens. Feed intake was the highest in group 2 (20 g/kg), female live weight was the highest in group 2 (20 g/kg) and 3 (30 g/kg). In conclusion, the rates of the cress seed (0.05, 0.10 and 0.15%) that contain glucotropaeolin were not affected for feed additive on performance (especially live weight and live weight gain) and carcass parameters.

Keywords

Brassica, Glucosinolates, Broiler, Villus, Performance

References

• Betz JM, and Fox WD (1994). High-performance liquid chromatographic determination of glucosinolates in Brassica vegetables, In: Food phytochemicals I: Fruits and vegetables. ACS Symposium Series, American Chemical Society, Washington DC: pp 181-196.
• Botsoglou NA, Florou-Paneri P, Christaki E, Fletouris AB, Spais DJ (2002). Effect of dietary oregano essential oil on performance of chickens and on iron- induced lipid oxidation of breast, thigh and abdominal fat tissues. British Poultry Science 43, 223-230.
• Brignall MS (2001). Prevention and Treatment of Cancer with Indole-3-Carbinol. Alternative Medicine Review 6, 580-589.
• Crossmann G (1937). A modification of Mallory's connective tissue stain with a discussion of the principles involved. Anatomical Record 69, 33–38.
• Diwakar BT, Dutta PK, Lokesh BR, Naidu KA (2010). Physicochemical properties of garden cress (Lepidium sativum L.) seed oil. Journal of the American Oil Chemists’ Society 87, 539-548.
• Fahey JW, Zalcmann AT, Talalay P (2001). The chemical diversity and distribution of glucosinolates and isothiocyanates among plants. Phytochemistry 56, 5-51.
• Gardner JJ, Adams NR (1986). The effect of zeranol and testesterone on merino wethers exposed to highly oestrogenic subtterranean clover pasture. Australian Veterinary Journal 63, 188-190.
• Giachetto PF, Guerreiro EN, Ferro JA, Ferro MIT, Furlan RL, Macari M (2003). Performance and hormonal profile in broiler chickens fed with different energy levels during post restriction period. Pesquisa Agropecuária Brasileira, 38, 697-702.
• Hendricks GL, Hadley JA, Krzysik-Walker SM, Prabhu KS, Vasilatos-Younken R, Ramachandran R (2009). Unique profile of chicken adiponectin, a predominantly Heavy molecular weight multimer and relationship to visceral adiposity. Endocrinology 150, 3092-3100.
• Herxheimer GW (1903). Sudan Black B method for fat. Zbl Allg Path Anat 14, 481.
• Jadhav NV, Awati B, Kulkarni S, Waghmare PG, Suranagi MD, Saxenam J, Ravikanth K, Dandale M and Maini S (2013). Performance of layer birds supplemented with herbal antistress product ayucee and synthetic vitamin C under physiological heat stress. Mal Jornal of Animal Science 16, 67-78.
• Jaiswal D, Kumar RP, Kumar A, Mehta S, Watal G (2009). Effect of Moringa oleifera Lam. leaves aqueous extract therapy on hyperglycemic rats. Journal of Ethnopharmacology 123, 392-396. Ke PJ, Ackman RG, Linke BA, Nash DM (1977). Differential lipid oxidation in various parts of frozen mackerel. Jornal of Food Technology 12, 37-47.
• Polat U (2010). The Effects on Metabolism of Glucosinolates and Theirs Hydrolysis Products. Journal of Biology and Environmental Science 4, 39-42.
• Rahman KM, Sarkar FH (2002). Steroid hormone mimics: Molecular mechanismsof cell growth and apoptosis in normal and malignant mammary epithelial cells. Journal of Steroid Biochemical Moleculer Biololgy 80, 191-201.
• Souri E, Amin GH, Farsam H, Andaji S (2004). The antioxidant activity of some commonly used vegetables in Iranian diet. Fitoterapia 75, 585-588.
• Talalay P, Fahey JW (2001). Phytochemicals from cruciferous plants protect against cancer by modulating carcinogen metabolism. Journal of Nutrition 131, 3027-33.
• Bialy Z, Oleszek W, Lewis J, Fenwick GR (1990). Allelopathic potential of glucosinolates (mustard oil glycosides) and their degradation products against wheat. Plant and Soil 129, 277-281.
• Zia-Ul-Haq M, Ahmad S, Calani L, Mazzeo T, Del Rio D, Pellegrini N, De Feo V (2012). Compositional study and antioxidant potential of Ipomoea hederacea acq. and Lepidium sativum L. seeds. Molecules 17, 10306-10321.