Changes of Photosynthetic Pigment Content in Lichens Collected from Urban and Rural Localities in Bursa Province

Year 2019, Volume: 13 Issue: 38, 65 - 69, 01.11.2019

Feyza Akyiğit Mesut Demir Şule Öztürk , Seyhan Oran

Abstract

In this study, contents of photosynthetic pigment in foliose Melanelixia subaurifera, Parmelia sulcata and fruticose Evernia prunastri, Ramalina farinacea were compared. Lichen species were collected from five localities in Bursa. Chlorophyll a, chlorophyll b, total chlorophlly, total carotenoid contents (mg/g), chlorophyll a/b ratio, total carotenoid/total chlorophyll ratio and phaeophytinization (OD435/OD415) ratio in the lichen extracts were differently determined between localities. These changes were found statistically significant (p≤0.05). The chlorophyll degradation rate at the localities in urban environments is determined to be higher than in the natural environment. It was observed that the air quality exposure ratio was related to the lichen morphology and thus the fruticose lichen species were more sensitive than foliose.

Keywords

Bursa, Chlorophyll degradation rate, Epiphytic lichen, Photosynthetic pigment content

References

• Bačkor M, Loppi S (2009). Interactions of lichens with heavy metals. Biol. Plantarum, 53: 214-222.
• Barnes JD, Balaguer L, Manrique E, Elvira S, and Davison AW (1992). A reappraisal of the use of DMSO for the extraction and determination of chlorophylls a and b in lichens and higher plants. Environ Exp Bot, 32: 85-100.
• Beeby A (2001). What do sentinels stand for? Environ Pollut, 112: 285-298.
• Garty J (2001). Biomonitoring Atmospheric Heavy Metals with Lichens: Theory and Application. Critical Reviews in Plant Sciences, 20(4): 309-371.
• Giordano S, Adamo P, Sorbo S, and Vingiani S (2005). Atmospheric trace metal pollution in the Naples urban area based on results from moss and lichen bags. Environmental Pollution, 136: 431-42.
• Güvenç Ş, and Bilgin A (2018). Which is the Better as an Indicator of Environmental Quality: Parmelia sulcata Taylor or Parmelina tiliacea (Hoffm.) Hale. Journal of Biological & Enviromental Sciences 12(34): 1-6.
• Güvenç Ş, Yıldız G, and Dere E (2018). Physiological Responses Of Epiphytic Lichens To The Urban And Rural Environment In The City Of Bursa (Turkey). AKU J. Sci. Eng. 18: 33-43.
• Hultengren S, Gralén H, and Pleijel H (2004). Recovery of the Epiphytic Lichen Flora Following Air Quality Improvement in South-West Sweden. Water, Air, and Soil Pollution, 154: 203-211.
• Kalinowska R, Bačkor M, and Pawlik-Skowrońsks B (2015). Parietin in the tolerant lichen Xanthoria parietina (L.) Th. Fr. increases protection of Trebouxia photobionts from cadmium excess. Ecological Indicators, 58: 132-138.
• Karakaş VE, Öztürk Ş, and Oran S (2017). Comparison of Photosynthetic Pigment Contets in Lichen Samples were Collected from Different Localities in Bursa. Journal of Biological & Enviromental Sciences, 11(33): 121-127.
• Kershaw KA (1985). Physiological Ecology of Lichens. Cambridge University Press, Cambridge.
• Llop E, Pinho P, Matos P, Pereira MJ, Branquinho C (2012). The used of lichen functional groups as indicators of air quality in a Mediaterranean urban enviroment. Ecological Indicators, 13: 215-221.
• Loppi S (2014). Lichens as sentinels for air pollution at remove alpine. Environ Sci Pollut Res, 21: 2563-2571.
• Majumder S, Mishra D, Ram SS, Jana NK, Santran S, Sudarshan M, Chakraborty A (2013). Physiological and chemical response of the lichen, Flavoparmelia caperata (L.) Hale, to the urban enviroment of Kolkata, India. Environ Sci Pollut Res, 20: 3077-3085.
• Munzi S, Pirintsos SA, and Loppi S (2009). Chlorophyll degradation and inhibition of polyamine biosynthesis in the lichen Xanthoria parietina under nitrogen stress. Ecotoxicology and Environmental Safety, 72: 281-285.
• Munzi S, Pisani T, Paoli L, and Loppi S (2010). Time- and dose- dependency of the effects of nitrogen pollution on lichens. Ecotoxicology and Environmental Safety, 73: 1785–1788.
• Paoli L, and Loppi S (2008). A biological method to monitör early effects of the air pollution caused by the industrial exploitation of geothermal energy. Environmental Pollution, 155: 383-388.
• Paoli L, Pisani T, Guttova A, Sardella G, and Loppi S (2011). Physiological and chemical response of lichens transplanted in and around an industrial area of south Italy: relationship with the lichen diversity. Ecotoxicology and Environmental Safety, 74: 650-657.
• Pirintsos SA, Paoli L, Loppi S, and Kotzabasis K (2011). Photosynthetic performance of lichen transplants as early indicator of climatic stress along an altitudinal gradient in the arid Mediaterranean area. Climatic Change, 107: 305-328.
• Ronen R, and Galun M (1984). Pigment extraction from lichens with dimethylsulfoxide (DMSO) and estimation of chlorophyll degradation. Environ Exp Bot, 24: 239-45.
• Seed L, Wolseley P, Gosling L, Davies L, and Power SA (2013). Modelling relationships between lichen bioindicators, air quality and climate on a national scale: Results from the UK OPAL air survey. Environmental Pollution, 182: 437-447.
• Sen G, Eryilmaz IE, and Ozakca D (2014). The effect of aluminium-stress and exogenous spermidine on chlorophyll degradation, glutathione reductase activity and the photosystem II D1 protein gene (psbA) transcript level in lichen Xanthoria parietina. Photochemistry, 98: 54-59.
• Sujetoviene G, and Galinyte V (2016). Effects of the urban environmental conditions on the physiology of lichen and moss. Atmospheric Pollution Research, 7: 611-618.
• Vannini A, Guarnieri M, Bačkor M, Bilová and Loppi S (2015). Uptake and toxicity of glyphosate in the lichen Xanthoria parietina (L.) Th. Fr. Ecotoxicology and Environmental Safety, 122: 193-197.
• Vannini A, Paoli L, Vichi M, Bačkor M, Bačkorova M, and Loppi S (2018). Toxicity of Diclofenac in the Fern Azolla filiculoides and the Lichen Xanthoria parietina. Bulletin of Environmental Contamination and Toxicology, 100: 430-437.
• Wellburn AR (1994). The spectral determination of chlorophylls a and b, as well as total carotenoids using various solvents with spectrophotometers of different resolution. J Plant Physiol, 144: 307-13.
• Will-Wolf S, Jovan S, and Amacher MC (2017). Lichen elemantal content bioindicators fora ir quality in upper Midwest, USA: A model for large-scale monitoring. Ecological Indicators, 78: 253-263.