Deneysel Epileptik Nöbet Modelinde, Artemisinin'in Fare Kalp ve Akciğer Dokularında Oksidatif Stress Belirteçleri Üzerine Etkisi.

Yıl 2022, Cilt: 12 Sayı: 6, 1013 - 1018, 30.11.2022

Yılmaz Koçak , Zübeyir Huyut , Fikret Türkan , Oruc Allahverdiyev , Bahattin Bulduk , Ugur Ozdek

https://doi.org/10.16899/jcm.1189565

Öz

Amaç: Çalışmamızın amacı, kalp ve akçiğer doku homojenatlarında malondialdehit (MDA) ve advanced oxidation protein products (AOPP), catalase (CAT), glutathione (GSH) ve glutathione peroxidase (GSH-Px) düzeylerinin farelerde deneysel PTZ-kindling modelinde oluşan nöbetlere karşı etkisi araştırılmıştır.
Gereç ve Yöntem: Deneyde Swiss albino erkek fareler (n=42) kullanıldı. Hayvanlar Kontrol, pentilenetetrazol 35 mg/kg (PTZ) , valproat 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS),+ PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ olmak üzere altı gruba ayrıldı. Deneyin 26. gününde farelrer sakrifiye edilerek, Biyokimyasal analizler için numuneler -80 0C'de muhafaza edildi.
Bulgular: Kalp ve akciğer dokularında analiz edilen beş biyokimyasal parametrede anlamlı farklar vardı. PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu ( p < 0.05). PTZ grubunun, Kalp ve akciğer MDA düzeyleri C ve ARS-60 grubuna göre anlamlı olarak yüksek bulundu. Aynı şekilde kalp'te AOPP düzeyleri VPA ve ARS-60 gruplarında anlamlı bir azalış sergilerken ( p < 0.05), akçiğerde ise gruplar arasında anlamlı bir fark yoktu ( p > 0.05). PTZ grubunun kalp'te CAT ve GSH düzeyleri diğer gruplara göre azalırken, akciğerde CAT düzeyi artığı, GSH düzeyininde azaldığı bulundu. kalp dokusundaki GSH-Px düzeyinde gruplar arasında anlamlı bir fark bulunmadı (p >0.05). GSH-Px düzeyi, akciğerde sadece ARS-30 grubunda analmlı artış göstermiştir (p < 0.05).
Sonuç: Sonuç olarak, PTZ işlem görmüş farelerde oluşan nöbertlerin periferal dokularda oksidatif strese neden olur. ARS tedavisi antioksidan savunma sistemine katkı sağlayabilir.

Anahtar Kelimeler

Artemisinin, Pentilentetrazol, Oksidatif stres, Antioksidan, Kalp, Akciğer

Destekleyen Kurum

YOK

Proje Numarası

YOK

Teşekkür

YOK

The Effect of Artemisinin on Oxidative Stress Markers in Mouse Heart and Lung Tissues in an Experimental Model of Epileptic Seizure.

Öz

Aim: The current study investigated the effects of artemisinin on the heart and lung tissue against pentylenetetrazol-induced seizures in mice. For this purpose, malondialdehyde (MDA), advanced oxidation protein products (AOPP), Catalase (CAT), glutathione (GSH), and glutathione peroxidase (GSH-Px) levels were evaluated in both tissue homogenates.

Material and Method: Swiss albino male mice (n=42) were used in the experiment. Animals were divided into six groups; Control (C), pentylenetetrazol (PTZ), valproate 100 mg/kg (VPA), artemisinin 30 mg/kg (ARS)+PTZ, ARS 60 mg/kg+PTZ, ARS 120 mg/kg+PTZ. On the 26th day of the experiment, the mice were sacrificed and the samples were kept at -80 0C for biochemical analysis.

Results: There were significant differences in the five biochemical parameters analyzed in heart and lung tissues. Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups (p<0.05). Heart and lung MDA levels of the PTZ group were found to be significantly higher than the C and ARS-60 groups. Likewise, heart AOPP levels decreased significantly in the VPA and ARS-60 groups compared to the PTZ group (p<0.05). There was no significant difference between the groups in terms of lung AOPP levels (p>0.05). Heart CAT and GSH levels were decreased in the PTZ group compared to the other groups. However, in terms of Lung CAT levels, the PTZ group had the highest value compared to the other groups, while it had the lowest value in terms of GSH level. The GSH-Px level did not differ significantly between the groups in heart tissue (p>0.05). The lung GSH-Px level was significantly increased in the ARS-30 group when compared to the PTZ group (p<0.05).

Conclusion: Consequently ARS treatment can inhibit PTZ-induced oxidative stress in peripheral tissues. In addition, ARS may provide improvements in decreased antioxidant enzymes. ARS may contribute to the antioxidant defense system.

Anahtar Kelimeler

Artemisinin, Pentylenetetrazol, oxidative stress, Antioxidant, Heart, Lung

Proje Numarası

YOK

Kaynakça

• Villa C, Lavitrano M, Combi R. Long Non-Coding RNAs and Related Molecular Pathways in the Pathogenesis of Epilepsy. Int J Mol Sci 2019;20(19):4898.
• Yuen AWC, Keezer MR, Sander JW. Epilepsy is a neurological and a systemic disorder. Epilepsy Behav 2018;78:57–61.
• Qi Z, Yu X, Xu P, Hao Y, Pan X, Zhang C. l-Homocarnosine, l-carnosine, and anserine attenuate brain oxidative damage in a pentylenetetrazole-induced epilepsy model of ovariectomized rats. 3 Biotech 2018;8(8):1–6.
• Yuan X, Fu Z, Ji P, et al. Selenium nanoparticles pre-treatment reverse behavioral, oxidative damage, neuronal loss and neurochemical alterations in pentylenetetrazole-induced epileptic seizures in mice. Int J Nanomedicine 2020;15:6339–53.
• Ahmed Amar SA, Eryilmaz R, Demir H, Aykan S, Demir C. Determination of oxidative stress levels and some antioxidant enzyme activities in prostate cancer. Aging Male 2019;22(3):198–206.
• Sheng F, Chen M, Tan Y, et al. Protective effects of Otophylloside N on Pentylenetetrazol-induced neuronal injury in vitro and in vivo. Front Pharmacol 2016;7:1–10.
• Nascimento FA, Tseng ZH, Palmiere C, et al. Pulmonary and cardiac pathology in sudden unexpected death in epilepsy (SUDEP). Epilepsy Behav 2017;73:119–25.
• Li ZP, Zhang XY, Lu X, Zhong MK, Ji YH. Dynamic release of amino acid transmitters induced by valproate in PTZ-kindled epileptic rat hippocampus. Neurochem Int 2004; 44: 263-70.
• Cheong DHJ, Tan DWS, Wong FWS, Tran T. Anti-malarial drug, artemisinin and its derivatives for the treatment of respiratory diseases. Pharmacol Res 2020;158:104901.
• Kim WS, Choi WJ, Lee S, et al. Anti-inflammatory, Antioxidant and Antimicrobial Effects of Artemisinin Extracts from Artemisia annua L. Korean J Physiol Pharmacol 2014;19:21–7.
• Ferreira JF, Luthria DL, Sasaki T, Heyerick A. Flavonoids from Artemisia annua L. as antioxidants and their potential synergism with artemisinin against malaria and cancer. Molecules 2010; 29;15:3135-70.
• Al-Humaidhi AM, Abd AH, Ghazi HF. Artemisinin alleviates pentylenetetrazole-induced kindling in male mice: involvement of gephyrin targeting. Eurasia J Biosci 2020;14:6457-63.
• Nasiri-Boroujeni S, Rahimi-Madiseh M, Lorigooini Z, Piroti K, Rafieian-Koupaei M, Amini-Khoei H. NMDA Receptor Mediates the Anticonvulsant Effect of Hydroalcoholic Extract of Artemisia persica in PTZ-Induced Seizure in Mice. Evid Based Complement Alternat Med 2021; 4:6422451.
• Singh SK, Dwivedi H, Gunjan S, Chauhan BS, Pandey SK, Tripathi R. Potential role of arteether on N-methyl-D-aspartate (NMDA) receptor expression in experimental cerebral malaria mice and extension of their survival. Parasitology 2019;146:1571-77.
• Ilhan A, Gurel A, Armutcu F, Kamisli S, Iraz M. Antiepileptogenic and antioxidant effects of Nigella sativa oil against pentylenetetrazol-induced kindling in mice. Neuropharmacology, 2005; 49: 456-64.
• Ohkawa H, Ohishi N, Yagi K. Assay for lipid peroxides in animal tissues by thiobarbituric acid reaction. Anal Biochem 1979;95(2):351–58.
• Lartillot S, Kedziora P, Athias A. Purification And Characterization Of A New Fungal Catalase. Prep Biochem 1988;18(3):241–46.
• Beutler E, Kelly BM. The effect of sodium nitrite on red cell GSH. Experientia 1963;19(2):96–97.
• Lawrence RA, Burk RF. Glutathione peroxidase activity in selenium-deficient rat liver. Biochem Biophys Res Commun 1976;71(4):952–58.
• Witko-Sarsat V, Gausson V, Descamps-Latscha B. Are advanced oxidation protein products potential uremic toxins? Kidney Int 2003;63(84):11–4.
• Oktay S, Bayrak G, Alev B, et al. The effect of vitamin U on the lung tissue of pentyleneterazole-induced seizures in rats. Naunyn Schmiedebergs Arch Pharmacol 2018;391:177–84.
• Goldman AM. Mechanisms of sudden unexplained death in epilepsy. Curr Opin Neurol 2015; 28(2):166–74.
• Kapucu A, Kaptan Z, Akgun Dar K, Kaleler İ, Uzum G. Effects of Erythropoietin Pretreatment on Liver, Kidney, Heart Tissue in Pentylentetrazol-Induced Seizures; Evaluation in Terms of Oxidative Markers, Prolidase and Sialic Acid. Istanbul Tip Fak Derg 2021;84(4):464–71.
• Dong YN, Hsu FC, Koziol-White CJ, et al. Functional NMDA receptors are expressed by human pulmonary artery smooth muscle cells. Sci Rep 2021;11(1):8205.
• Anaparti V, Ilarraza R, Orihara K, et al. NMDA receptors mediate contractile responses in human airway smooth muscle cells. Am J Physiol Lung Cell Mol Physiol 2015; 308:1253-64.
• Zaidi SMKR, Al-Qirim TM, Banu N. Effects of Antioxidant Vitamins on Glutathione Depletion and Lipid Peroxidation Induced by Restraint Stress in the Rat Liver. Drugs R D 2012; 6(3):157–65.
• Tambe R, Patil A, Jain P, Sancheti J, Somani G, Sathaye S. Assessment of luteolin isolated from Eclipta alba leaves in animal models of epilepsy. Pharm Biol 2017;55(1):264-68.
• Huang XT, Liu W, Zhou Y, Hao CX, Zhou Y, Zhang CY, Sun CC, Luo ZQ, Tang SY. Dihydroartemisinin attenuates lipopolysaccharide‑induced acute lung injury in mice by suppressing NF‑κB signaling in an Nrf2‑dependent manner. Int J Mol Med 2019;44:2213-22.
• Liu, Z., Zhang, J., Li, S. et al. Artesunate Inhibits Renal Ischemia Reperfusion-Stimulated Lung Inflammation in Rats by Activating HO-1 Pathway. Inflammation 2018;41:114–21.
• Zhang, H., Qi, S., Song, Y., Ling, C. Artemisinin attenuates early renal damage on diabetic nephropathy rats through suppressing TGF-β1 regulator and activating the Nrf2 signaling pathway. Life sciences 2020;256:117966.
• Ruiz-Ojeda FJ, Olza J, Gil Á, Aguilera CM. Oxidative Stress and Inflammation in Obesity and Metabolic Syndrome. In: Del Moral AM, Aguilera CM, editors. Obesity: Oxidative Stress and Dietary Antioxidants. Philadelphia: Elsevier; 2018. p. 1–15.
• Escasany E, Izquierdo-Lahuerta A, Medina-Gómez G. Kidney Damage in Obese Subjects: Oxidative Stress and Inflammation. In: Del Moral AM, Aguilera C, editors. Obesity: Oxidative Stress and Dietary Antioxidants. Philadelphia: Elsevier; 2018. p. 135–62.
• Bayram S, Türkyılmaz İB, Karaman GB,Yanardağ R. Effects of S-Methyl Methionine Sulfonium Chloride on Lens Tissue in Pentylenterazol-Induced Seizures in Rats. Arch Epilepsy 2022; 28(3):98-105. Aktaş I, Özmen O, Tutun H, Yalçın A, Türk A. Artemisinin attenuates doxorubicin induced cardiotoxicity and hepatotoxicity in rats. Biotech Histochem 2020;95(2):121–8.
• Dirik D, Kömüroğlu AU. The effect of infliximab on oxidative stress in ovarian tissue in the rat of ovarian hypersrimulation syndrome. East J Med 2021;26(3):475–80.
• Çelikezen FÇ, Oto G, Özdemir H, et al. The antioxidant effect of boric acid and CoQ10 on pulmonary fibrosis in bleomycin induced rats. Bitlis Eren Univ J Sci Technol 2015;2(1):27–31.
• Kumar V, Sharma SK, Nagarajan K, Dixit PK. Effects of lycopene and sodium valproate on pentylenetetrazol-induced kindling in mice. Iran J Med Sci 2016;41(5):430–36.
• Saha L, Chakrabarti A, Kumari S, Bhatia A, Banerjee D. Antiapoptotic and neuroprotective role of curcumin in pentylenetetrazole (PTZ) induced kindling model in rat. Indian J Exp Biol 2016;54(2):133–41.
• Taiwe GS, Moto FCO, Ayissi ERM, et al. Effects of a lyophilized aqueous extract of Feretia apodanthera Del. (Rubiaceae) on pentylenetetrazole-induced kindling, oxidative stress, and cognitive impairment in mice. Epilepsy Behav 2015;43:100–08.
• Hamed SA. The effect of antiepileptic drugs on the kidney function and structure. Expert Rev Clin Pharmacol 2017;10(9):993–1006.
• Wang N, Zhang M, Ma Y, et al. Fluorescent nanodiamonds as enzyme mimics for protecting astrocytes from oxidative stress in a mouse model of epilepsy. J Nanoparticle Res 2021; 23(12):1–12.
• Abdel-Salam OME, Sleem AA, Sayed MAEBM, Youness ER, Shaffie N. Capsaicin Exerts Anti-convulsant and Neuroprotective Effects in Pentylenetetrazole-Induced Seizures. Neurochem Res 2020:45(5);1045–61.
• Singh N, Vijayanti S, Saha L, Bhatia A, Banerjee D, Chakrabarti A. Neuroprotective effect of Nrf2 activator dimethyl fumarate, on the hippocampal neurons in chemical kindling model in rat. Epilepsy Res 2018;143:98–104.
• Dillioglugil MO, Kir HM, Demir C, et al. Effect of pentylenetetrazole and sound stimulation induced single and repeated convulsive seizures on the MDA, GSH and NO levels, and SOD activities in rat liver and kidney tissues. Brain Res Bull 2010;83(6):356–59.
• M. Faheem HM, M. Elnbtete SM. Artemisia annua Extract Ameliorates DMBA-induced Breast Cancer in Albino rats; Antioxidant and Genetic Effects. Orient J Chem 2020:36(03);451–7.