Evaluation of the Effect of the Combination of Boron Compounds on Chronic Liver Disease

Yıl 2023, Cilt: 13 Sayı: 2, 163 - 169, 22.03.2023

Özgür Çelebi , Demet Çelebi , Ali Taghizadehghalehjoughi , Sümeyye Başer , Mustafa Can Güler , Serkan Yıldırım

https://doi.org/10.16899/jcm.1176975

Cited By: 1

Öz

Aim: Enterococcus faecalis has surface adhesion proteins that enable it to attach to human intestinal and vaginal tissue cells with antibiotic-resistant strains in patients. Due to these properties, boron and its derivatives are preferred as therapeutic agents due to their antibacterial, antifungal, antiparasitic and antifungal activities. In this study, we aimed to evaluate the synergistic effect of boron compounds and their effect on biofilms in an infection model created with Enterococcus faecalis 29212 on the HepG2 liver cell line.
Materials and Methods: It was determined that sodium perborate monohydrate + zinc borate had the lowest values as a result of the minimum inhibitory concentration and fractional inhibitor concentration studies. It has also been shown that these doses reduce cytotoxic effects. In addition, 32 µg/ml Etidote + 256 µg/ml Sodium Perborate Monohydrate showed the highest biofilm effect.
Results: we show that boron compounds effectively reduce biofilm formation and cause the death of bacteria.

Anahtar Kelimeler

Biofilm, Boron Compounds, Fractional Inhibition Concentration, Cell Culture, Synergistic Effect

Bor Bileşikleri Kombinasyonunun Kronik Karaciğer Hastalığı Üzerindeki Etkisinin Değerlendirilmesi

Öz

Amaç: Enterococcus faecalis, hastalarda antibiyotiğe dirençli suşlarla insan bağırsak ve vajinal doku hücrelerine bağlanmasını sağlayan yüzey yapışma proteinlerine sahiptir. Bu özelliklerinden dolayı bor ve türevleri antibakteriyel, antifungal, antiparaziter ve antifungal aktivitelerinden dolayı terapötik ajanlar olarak tercih edilmektedir. Bu çalışmada, HepG2 karaciğer hücre hattı üzerinde Enterococcus faecalis 29212 ile oluşturulan bir enfeksiyon modelinde bor bileşiklerinin sinerjistik etkisini ve biyofilmler üzerindeki etkisini değerlendirmeyi amaçladık.
Gereç ve Yöntem: Minimum inhibitör konsantrasyonu, fraksiyonel inhibitör konsantrasyonu ve biyofilm çalışmaları ve HepG2 hücre analizleri yapılarak değerlendirildi.
Bulgular: Minimum inhibitör konsantrasyonu ve fraksiyonel inhibitör konsantrasyonu çalışmaları sonucunda sodyum perborat monohidrat + çinko boratın en düşük değerlere sahip olduğu belirlendi. Bu dozların sitotoksik etkileri azalttığı da gösterilmiştir. Ayrıca en yüksek biyofilm etkisini 32 µg/ml Etidot + 256 µg/ml Sodyum Perborat Monohidrat göstermiştir.
Sonuç: Bor bileşiklerinin biyofilm oluşumunu etkili bir şekilde azalttığını ve bakterilerin ölümüne neden olduğunu gösterdik.

Anahtar Kelimeler

Biyofilm, Bor Bileşikleri, Fraksiyonel İnhibisyon Konsantrasyonu, Hücre Kültürü, Sinerjik Etki, Biyofilm, Bor Bileşikleri, Fraksiyonel İnhibisyon Konsantrasyonu, Hücre Kültürü, Sinerjik Etki

Kaynakça

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