Odontojenik Kistlerde Plazma Hücresi ve Makrofajların Dağılımı

Yıl 2023, Cilt: 13 Sayı: 6, 1076 - 1080, 30.11.2023

Özge Kaya Korkmaz , Havva Erdem , Mürüvvet Akçay Çelik , Fadime Gül Salman , Büşra Erşan Erdem , Yeliz Kaşko Arıcı

Öz

Özet
Amaç : Odontojenik kistler maksillofasiyal bölgenin sık karşılaşılan önemli lezyonlarıdır. Radiküler kistler, dentijeröz kistler ve odontojenik keratokistler en sık karşılaşılan odontojenik kistlerdir. Makrofajlar ve plasma hücreleri inflamasyonun temel hücreleridir ve bir çok hastalığın oluşumunda rol oynarlar.
Bu çalışmanın amacı; en sık karşılaşılan odontojenik kistlerde makrofaj ve plazma hücrelerinin varlığını ve dağılımını klinik bilgilerle birlikte karşılaştırmak amaçlanmıştır.
Metod: Çalışmamıza laboratuvarımızda Ocak 2013 ile Aralık 2022 tarihleri arasında odontojenik kist tanısı almış, eksizyonel biyopsi uygulanmış olgular dahil edildi. Olgulara ait Hematoksilen-Eozin boyalı kesitler değerlendirildi. İmmünhistokimyasal (İHK) boyama için en uygun olan alanlar işaretlendi. Daha sonra parafin bloklardan 2 mm çapta silindirik şekilli parafinize doku örnekleri manuel mikroarray cihazı ile donör bloklardan alınarak çoklu bloklara aktarıldı. Anti- CD68 ve anti- CD138 immünhistokimyasal boyası çoklu bloklara uygulandı. Boyalı preparatlar ortalama bir skor verilerek 0-2 arasında puanlandı. Daha sonra verilen skorlar 3 grup arasında klinik verilerle birlikte analiz edildi.
Bulgular: Çalışmamıza dahil edilen 83 odontojenik kistin 41 tanesi radiküler kist, 25 tanesi dentijeröz kist ve 17 tanesi keratokistti. Hastaların yaşları 17 ile 77 arasında değişiyordu ve ortalama 37,55± 16,42’ idi. Hastaların %47’si erkek iken % 53 kadındı. Radiküler kist, dentijeröz kist ve keratokist grupları arasında yaş ve cinsiyet açısından anlamlı fark yoktu (p>0.05).
Kist tipi ile CD68+ makrofajların ve CD138+ plasma hücrelerinin oranları arasında anlamlı fark saptandı. (p<0.05).
Sonuç : Odontojenik kist tipleri arasında CD68+ makrofajların ve CD138 + plasma hücrelerinin dağılımında anlamlı fark saptanmıştır.Bu nedenle odontojenik kistlerin histomorfolojik özellikleri hakkında daha fazla bilgi sahip olmak ve inflamatuar süreçlerini anlamak doğru tanı ve tedavi için önem arz etmektedir.

Anahtar Kelimeler

Radiküler kist, Odontojenik kist, Makrofaj, Plazma hücresi

Destekleyen Kurum

yok

Proje Numarası

yok

Teşekkür

yok

Distribution of Macrophages and Plasma Cells in Odontogenic Cysts

Öz

Abstract
Objective : Odontogenic cysts are common and important lesions of the maxillofacial region. Radicular cysts, dentigerous cysts, and odontogenic keratocysts are the most common odontogenic cysts. Macrophages and plasma cells are the main cells of inflammation and play a role in the development of many diseases.
This study aimed to compare the presence and distribution of macrophages and plasma cells among the most common odontogenic cysts with clinical data.
Method : Cases diagnosed with odontogenic cysts in our laboratory were included in our study. Hematoxylin-Eosin stained sections of the cases in the archive were re-evaluated. The area that best reflected the inflammation tissue was first marked on the slides and then on the blocks. Then, 2 mm diameter cylindrical-shaped paraffinized tissue samples were taken from donor blocks and transferred to multiple blocks with a manual microarray device. Anti-CD68 and anti-CD138 immunohistochemical stains were applied to multiple blocks. The stained preparations were scored between 0-2 by giving an average score. The scores were then analyzed together with clinical data between the three groups.
Results: Of the 83 odontogenic cysts included in our study, 41 were radicular cysts, 25 were dentigerous cysts, and 17 were keratocysts. The ages of the patients ranged from 17 to 77 years, with a mean of 37.55± 16.42 years. 47% of the patients were male, and 53% were female. There was no significant difference between the odontogenic cyst groups regarding age and gender (p>0.05).
There was a significant difference between the cyst type and the proportions of CD68+ macrophages and CD138+ plasma cells (p<0.05).
Conclusion: There was a significant difference in the distribution of CD68+ macrophages and CD138+ plasma cells in odontogenic cyst type. Therefore, it is important to have more information about the histomorphologic features of odontogenic cysts and to understand their inflammatory processes for correct diagnosis and treatment.

Anahtar Kelimeler

Radicular cyst, odontogenic cyst, macrophage, plasma cells.

Proje Numarası

yok

Kaynakça

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